Isolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis

Malik, Minnie; Wang, Zhen; Dirpaul, Joan M.; Zhou, Ning; Hammerlindl, Joe; Keller, W. A.; Abrams, Suzanne R.; Ferrie, A. M. R.; Krochko, Joan E.

doi:10.1093/jxb/ern149

Cited by 41 publications

(34 citation statements)

References 72 publications

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“…Indeed, the ectopic expression of key regeneration regulators, such as WUS and WIND1, has already been shown to promote organ regeneration and/or somatic embryogenesis in various crops (Srinivasan et al, 2007;Arroyo-Herrera et al, 2008;Heidmann et al, 2011;Iwase et al, 2013Iwase et al, , 2015Florez et al, 2015). Expression profiles of key regeneration regulators have also been used in crops to identify cultivars with high regeneration capacities (Malik et al, 2008). Further mechanistic understanding of plant regeneration should help us to advance the classic but not fully exploited field of tissue culture, with numerous downstream implications for both basic and applied biology.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

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Compared with animals, plants generally possess a high degree of developmental plasticity and display various types of tissue or organ regeneration. This regenerative capacity can be enhanced by exogenously supplied plant hormones in vitro, wherein the balance between auxin and cytokinin determines the developmental fate of regenerating organs. Accumulating evidence suggests that some forms of plant regeneration involve reprogramming of differentiated somatic cells, whereas others are induced through the activation of relatively undifferentiated cells in somatic tissues. We summarize the current understanding of how plants control various types of regeneration and discuss how developmental and environmental constraints influence these regulatory mechanisms.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

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“…Brassinosteroids have also shown positive effects in increasing the androgenic response in highly recalcitrant rapeseed genotypes ('Westar', for example), in a stable and inheritable way (Malik et al 2008). In other words, the use of brassinosteroids may both increase SAM quality and modify the androgenic response of a plant and its sexual offspring.…”

Section: Androgenic Dhs In Peppermentioning

confidence: 99%

Androgenesis in recalcitrant solanaceous crops

et al. 2010

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 Abstract Tomato, eggplant and pepper are three solanaceous crops of outstanding importance worldwide. Thus, a fast and cheap method to produce pure (homozygous) lines is a priority for breeders. Traditionally, pure lines are produced by classical inbreeding and selection techniques, which are time consuming (several years) and costly. Alternatively, it has become possible to accelerate the production of homozygous lines through a biotechnological approach: the induction of androgenesis to generate doubled haploid (homozygous) plants. This biotechnological in vitro tool reduces the process to only one generation, which implies important time and costs savings. These facts make androgenic doubled haploids the choice in a number of important crops where the methodology is well set up. Unfortunately, recalcitrant solanaceous crops such as tomato, eggplant and pepper are still far from an efficient and reliable technology to be applied to different genotypes in breeding programs on a routine basis. In eggplant and pepper, only anther cultures are known to work relatively well. Unfortunately, a more efficient and promising technique, the culture of isolated microspores, is not sufficiently developed yet. In tomato, none of these methods is available. However, recent advances in the knowledge of zygotic and androgenic embryo development are filling the gaps and opening new ways to achieve the final goal of an efficient protocol in these three recalcitrant species. In this review, we outline the state of the art on androgenic induction in tomato, eggplant and pepper, and postulate new experimental ways in order to overcome current limitations.

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“…The widely studied double haploid line DH12075 is involved in many aspects of Brassica breeding and genomics; consequently, optimizing microspore culture for consistent embryogenesis would be beneficial. Westar is a highly transformable genotype but responds poorly in standard embryogenic conditions (Malik et al, 2008), so is an excellent candidate for embryogenicity improvement. The line 0025 has been reported as a model non-embryogenic genotype (Simmonds and Keller, 1999), and was included as a negative control.…”

Section: Introductionmentioning

confidence: 99%