Isolation of an exopolysaccharide-producing heavy metal-resistant Halomonas sp. MG

Govarthanan, Muthusamy; Shim, Jaesool; Praburaman, Loganathan; Kim, Seol Ah; Oh, Byung‐Taek

doi:10.1007/s00203-015-1173-5

Cited by 21 publications

(6 citation statements)

References 20 publications

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“…Interestingly, heavy metal tolerance of BRI 36 in terms of maximum tolerable concentration (MTC) was found to increase considerably with an increase in EPS produced by the isolate. Role of EPS in metal resistance has been previously documented by various researchers [14][15][16][17]. These observations prompted us to undertake studies on EPS production, and its effect on heavy metal tolerance of Kocuria sp.…”

Section: Introductionmentioning

confidence: 87%

Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

Mulik

Bhadekar

2018

Int J Pharm Pharm Sci

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Objective: Evaluation of Extracellular Polymeric Substance (EPS) induced heavy metal tolerance in Kocuria sp. BRI 36.Methods: Initially, the effect of different concentrations of glucose (1-10 %) on EPS production by BRI 36 was examined. At optimum glucose concentrations, EPS levels were measured by varying heavy metal concentrations (10-50 ppm) of Pb 2+ , Cd 2+ and Cr 3+Results: Kocuriasp. BRI 36 produced maximum EPS (1g/l) at 5% glucose. Increase in EPS production up to 89% (considering 1g/l as 100%) with an increase in concentrations of heavy metals up to 40 ppm. MTC levels of BRI 36 for heavy metals increased up to 700 ppm when it was cultivated in presence of 5% glucose indicating a major role of surface polymer in metal adsorption. The function of EPS as a protective cover was also evident from an increase in survival percentage of BRI 36 up to 39.4 at MTC. Comparative analysis of extracted crude EPS by FTIR revealed the involvement of O-H, C=O, and C-O-C groups in metal adsorption . Maximum tolerable concentration (MTC) and survival percentage of BRI 36 were determined under conditions that support EPS synthesis. Comparative analysis of extracted crude EPS was performed by Fourier Transform Infrared Spectroscopy (FTIR) to establish functional groups involved in the metal interaction. Conclusion:Antarctic oceanic isolate Kocuria sp. BRI 36 has an ability to produced EPS under stress conditions of heavy metals. Simultaneously, its MTC values increased due to increase in EPS levels. These observations suggest the possibility to develop gentle, environmentally safe and costeffective method for heavy metal removal.

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Section: Introductionmentioning

confidence: 87%

Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

Mulik

Bhadekar

2018

Int J Pharm Pharm Sci

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“…The FESEM was used to study the microstructures of the EPS. [ 35 ] The FESEM images showed multiple fissures and pores in the EPS microstructures (Figure 1B). Multiple reports indicated the porous microstructure nature of the EPS and suggested as potential thickeners, viscosifiers, texturizers, and eventually as stabilizing agents utilized in the food industry.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Rainbow Trout (Oncorhynchus mykiss) Collagen Incorporated with Exo‐Polysaccharides Derived from Rhodotorula mucilaginosa sp. on Burn Healing

Ghadimi

Gharahgheshlagh

Latifi

et al. 2023

Macromolecular Bioscience

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Burn is one of the physically debilitating injuries that can be potentially fatal; therefore, providing appropriate coverage in order to reduce possible mortality risk and accelerate wound healing is mandatory. In this study, collagen/exo‐polysaccharide (Col/EPS 1–3%) scaffolds are synthesized from rainbow trout (Oncorhynchus mykiss) skins incorporated with Rhodotorula mucilaginosa sp. GUMS16, respectively, for promoting Grade 3 burn wound healing. Physicochemical characterizations and, consequently, biological properties of the Col/EPS scaffolds are tested. The results show that the presence of EPS does not affect the minimum porosity dimensions, while raising the EPS amount significantly reduces the maximum porosity dimensions. Thermogravimetric analysis (TGA), FTIR, and tensile property results confirm the successful incorporation of the EPS into Col scaffolds. Furthermore,the biological results show that the increasing EPS does not affect Col biodegradability and cell viability, and the use of Col/EPS 1% on rat models displays a faster healing rate. Finally, histopathological examination reveals that the Col/EPS 1% treatment accelerates wound healing, through greater re‐epithelialization and dermal remodeling, more abundant fibroblast cells and Col accumulation. These findings suggest that Col/EPS 1% promotes dermal wound healing via antioxidant and anti‐inflammatory activities, which can be a potential medical process in the treatment of burn wounds.

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“…Field emission scanning electron microscopy was a known technique for studying the microstructure of EPSs. 32 As shown in Figure 2A-B, the EPSs micrographs are presented a porous and three-dimensional structure and several crevices were observed in their microstructure. There were many studies on microstructures of EPSs which some of them have reported the porous nature of EPS.…”

Section: Morphology and Water Holding Capacity Of Epssmentioning

confidence: 85%

“…There were many studies on microstructures of EPSs which some of them have reported the porous nature of EPS. [32][33][34] Ramirez suggested that EPS of porous nature can be used as a thickener, texturizer, viscosifier and stabilizing agent in food industry. 4 The water holding capacity of the EPSs was 519 ± 18.5%.…”

Section: Morphology and Water Holding Capacity Of Epssmentioning

confidence: 99%

Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

et al. 2020

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Purpose: Due to the potential industrial and therapeutic applications of the yeast exopolysaccharides (EPSs), there has been an increasing demand to assess these biopolymers with improved characteristics. This study aimed to characterize the EPSs from Rhodosporidium babjevae (ATCC 90942 and IBRC-M 30088) as well as to evaluate their possible antioxidant, emulsifying and antiproliferative activities. Methods: Rhodosporidium babjevae was cultured for 5 days and following isolation of supernatant, EPSs precipitated with adding of cold absolute ethanol and freeze-dried. The EPSs chemical structure was determined by FT-IR, SEM, HPLC-SEC and GC-MS. Additionally the solubility, water holding capacity and emulsifying activity of EPSs were evaluated. In vitro, antioxidant activity was investigated against DPPH, superoxide and hydroxyl radicals. Finally the EPSs consequence on the cell proliferation of human breast adenocarcinoma (MCF-7) and Madin-Darby canine kidney (MDCK) cell lines was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Results: R. babjevae excreted 1.6±0.2 g/L of the EPSs. The EPSs had three fractions with molecular weights of 1.02 ×106 , 5×105 and 2×105 Da. Mannose and glucose were found as the main monosaccharides of the EPSs (84:16 mol%, respectively). The EPSs exhibited emulsifying activity on sun flower oil. The scavenging activities were found to be dose-dependent and higher than hyaluronic acid. Significant difference among the EPSs treatments on the proliferation of MCF-7 and MDCK cell lines was not observed (P>0.05). Conclusion: These results show the interesting potential of the EPSs from R. babjevae as biocompatible compounds for using in food and pharmaceutical fields.

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Isolation of an exopolysaccharide-producing heavy metal-resistant Halomonas sp. MG

Cited by 21 publications

References 20 publications

Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

Extracellular Polymeric Substance (Eps) From Kocuria Sp. Bri 36: A Key Component in Heavy Metal Resistance

The Effect of Rainbow Trout (Oncorhynchus mykiss) Collagen Incorporated with Exo‐Polysaccharides Derived from Rhodotorula mucilaginosa sp. on Burn Healing

Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

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