Isolation of Arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing

Murayama, Maki; Hayashi, Shotaro; Nishimura, Noriyuki; Ishide, Mayumi; Kobayashi, Kenzo; Yagi, Yusuke; Asami, Tadao; Nakamura, Takahiro; Shinozaki, Kazuo; Hirayama, Takashi

doi:10.1093/jxb/ers188

Cited by 69 publications

(78 citation statements)

References 53 publications

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“…The PGN can regulate the ROS homeostasis in plants mitochondria during abiotic and biotic stress responses may occur through the regulation of mitochondria-nucleus retrograde signaling. AHG11 regulates the nad4 (mitochondrion complex I) transcriptional level and thus led to changes in oxidative levels by controlling RNA editing events in plants mitochondria and affecting plant responses to ABA [21]. SLG1 regulated the nad3 (mitochondrion complex I) transcript by regulating RNA editing events in mitochondria and affecting the expression of genes which were involved in the alternative respiratory pathway [22].…”

Section: Resultsmentioning

confidence: 99%

Genome-wide investigation and expression analyses of the pentatricopeptide repeat protein gene family in foxtail millet

Liu

et al. 2016

BMC Genomics

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BackgroundPentatricopeptide repeat (PPR) proteins are encoded by a large gene family of approximately 450 members in Arabidopsis and 477 in rice, which characterized by tandem repetitions of a degenerate 35 amino acid characteristic sequence motifs. A large majority of the PPR genes in the higher plants are localized in organelles. Their functions remain as yet largely unknown. The majority of characterized PPR proteins have been found to function in modulating the expression plastid and mitochondrial genes in plants.ResultsHere, a genome-wide identification and comparison of the PPR genes from 5 organisms was performed, including the moss Physcomitrella patens, the lycophyte Selaginella moellendorffii, the eudicot Arabidopsis, and the monocots rice and foxtail millet. It appears that the expansion of this gene family prior to the divergence of the euphyllophytes and the lycophytes in land plants. The duplication and divergence rates of the foxtail millet PPR genes (SiPPRs) showed that the expansion period of this gene family around 400 Mya, and indicated that genome segmental duplication was very likely the primary mechanism underlying the expansion of the PPR gene family in vascular plants. An analysis of a complete set of SiPPR genes/proteins that included classification, chromosomal location, orthologous relationships, duplication analysis, and auxiliary motifs is presented. Expression analysis of the SiPPR genes under stress conditions revealed that the expression of 24 SiPPR genes was responsive to abiotic stress. Subcellular localization analysis of 11 PPR proteins indicated that 5 proteins were localized to chloroplasts, that 4 were localized to mitochondria, and that 2 were localized to the cytoplasm.ConclusionsOur results contribute to a more comprehensive understanding the roles of PPR proteins and will be useful in the prioritization of particular PPR proteins for subsequent functional validation studies in foxtail millet.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3184-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Genome-wide investigation and expression analyses of the pentatricopeptide repeat protein gene family in foxtail millet

Liu

et al. 2016

BMC Genomics

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“…In contrast to the PP2C-encoding AHG1 and AHG3, AHG2 (AT1G55870), AHG11 (AT2G44880), ABH1 (AT2G13540), and SAD1 (AT5G48870) all encode enzymes involved in RNA processing or degradation (Hugouvieux et al, 2001;Nishimura et al, 2005;Murayama et al, 2012) that could affect RNA accumulation at a post-transcriptional step.…”

Section: Diverse Regulators With Pleiotropic Effectsmentioning

confidence: 99%

Abscisic Acid Synthesis and Response

Finkelstein¹

2013

The Arabidopsis Book

875

744

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Background: Small heat shock proteins (sHSPs) are critical for plant response to biotic and abiotic stresses, especially heat stress. They have also been implicated in various aspects of plant development. However, the acting mechanisms of the sHSPs in plants, especially in perennial grass species, remain largely elusive. Results: In this study, AsHSP26.8a, a novel chloroplast-localized sHSP gene from creeping bentgrass (Agrostis stolonifera L.) was cloned and its role in plant response to environmental stress was studied. AsHSP26.8a encodes a protein of 26.8kDa. Its expression was strongly induced in both leaf and root tissues by heat stress. Transgenic Arabidopsis plants overexpressing AsHSP26.8a displayed reduced tolerance to heat stress. Furthermore, overexpression of AsHSP26.8a resulted in hypersensitivity to hormone ABA and salinity stress. Global gene expression analysis revealed AsHSP26.8a-modulated expression of heat-shock transcription factor gene, and the involvement of AsHSP26.8a in ABAdependent and-independent as well as other stress signaling pathways. Conclusions: Our results suggest that AsHSP26.8a may negatively regulate plant response to various abiotic stresses through modulating ABA and other stress signaling pathways.

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“…Although lethal in animals, plants with disrupted complex I are usually viable and functionally rescued by the expression of alternative NDH. Plant mutants affected in the expression of nuclearas well as mitochondria-encoded complex I subunits display a diversity of phenotypes, such as germination deficiencies, growth retardation, developmental defects, and altered responses to hormones or stresses (Lee et al, 2002;Perales et al, 2005;de Longevialle et al, 2007;Zsigmond et al, 2008;Meyer et al, 2009;Murayama et al, 2012;Toda et al, 2012;Yuan and Liu, 2012;Haïli et al, 2013;Zhu et al, 2014). Plant complex I mutants have been largely used to study complex I architecture, respiration plasticity, and metabolism adaptation (Dutilleul et al, 2003;Noctor et al, 2004;Meyer et al, 2011;Braun et al, 2014).…”

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confidence: 99%

Disruption of the CYTOCHROME C OXIDASE DEFICIENT1 Gene Leads to Cytochrome c Oxidase Depletion and Reorchestrated Respiratory Metabolism in Arabidopsis

et al. 2014

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Cytochrome c oxidase is the last respiratory complex of the electron transfer chain in mitochondria and is responsible for transferring electrons to oxygen, the final acceptor, in the classical respiratory pathway. The essentiality of this step makes it that depletion in complex IV leads to lethality, thereby impeding studies on complex IV assembly and respiration plasticity in plants. Here, we characterized Arabidopsis (Arabidopsis thaliana) embryo-lethal mutant lines impaired in the expression of the CYTOCHROME C OXIDASE DEFICIENT1 (COD1) gene, which encodes a mitochondria-localized PentatricoPeptide Repeat protein. Although unable to germinate under usual conditions, cod1 homozygous embryos could be rescued from immature seeds and developed in vitro into slow-growing bush-like plantlets devoid of a root system. cod1 mutants were defective in C-to-U editing events in cytochrome oxidase subunit2 and NADH dehydrogenase subunit4 transcripts, encoding subunits of respiratory complex IV and I, respectively, and consequently lacked cytochrome c oxidase activity. We further show that respiratory oxygen consumption by cod1 plantlets is exclusively associated with alternative oxidase activity and that alternative NADH dehydrogenases are also up-regulated in these plants. The metabolomics pattern of cod1 mutants was also deeply altered, suggesting that alternative metabolic pathways compensated for the probable resulting restriction in NADH oxidation. Being the first complex IV-deficient mutants described in higher plants, cod1 lines should be instrumental to future studies on respiration homeostasis.

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Isolation of Arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing

Cited by 69 publications

References 53 publications

Genome-wide investigation and expression analyses of the pentatricopeptide repeat protein gene family in foxtail millet

Genome-wide investigation and expression analyses of the pentatricopeptide repeat protein gene family in foxtail millet

Abscisic Acid Synthesis and Response

Disruption of the CYTOCHROME C OXIDASE DEFICIENT1 Gene Leads to Cytochrome c Oxidase Depletion and Reorchestrated Respiratory Metabolism in Arabidopsis

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