Isolation of Auxotrophic Mutants of Allomyces macrogynus

Olson, Lauritz W.; Nielsen, Tom A. B.

doi:10.2307/3759602

Cited by 4 publications

(1 citation statement)

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“…These results suggest that Allo2 may be anisogamous; however, we have been unable to reproducibly induce the anisogamous hermaphroditic hyphae in the laboratory cultures of this strain. Further, the strain, Burma 3-35 was historically reported as being anisogamous (Olson and Nielsen 1981), but forms a well-supported clade in our analysis with the isogamous strain, Neo (Olson 1980) (Figure 1e, Figure 2, Figure S1). Burma 3-35 also shares most genes with the strain Neo (Figure 2a, Figure S2c) and has not shown anisogamous mating structures in laboratory cultures, even after repeated trials (data not shown).…”

Section: Figure 2 Evolution Of Anisogamy In Allomycesmentioning

confidence: 53%

Evolution of anisogamy in the early diverging fungus,Allomyces

Phadke

Rupp

Sayres

2017

Preprint

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Gamete size dimorphism between sexes (anisogamy) is predicted to have evolved from isogamous system in which sexes have equal-sized, monomorphic gametes. Although adaptive explanations for the evolution of anisogamy abound, we lack comparable insights into molecular changes that bring about the transition from monomorphism to dimorphism. The basal fungal clade Allomyces provides unique opportunities to investigate genomic changes that are associated with this transition in closely related species that show either isogamous or anisogamous mating systems.The anisogamous species show sexual dimorphism in gamete size, number, pigmentation and motility. We sequenced transcriptomes of five Allomyces isolates representing the two mating systems, including both sexual phenotypes in the anisogamous species. Maximum likelihood ancestral character state reconstruction performed in MESQUITE using the de-novo assembled transcriptomes indicated that anisogamy likely evolved once in Allomyces, and is a derived character as predicted in theory. We found that sexual stages of Allomyces expresses homologs of several genes known to be involved in sex determination in model organisms including Drosophila and humans. Furthermore, expression of CatSper homologs in male-and female-biased samples in our analysis support the hypothesis that gamete interaction in the anisogamous species of Allomyces may involve similar molecular events as the eggsperm interaction in animals, including humans. Although the strains representing either mating system shared much of the transcriptome, supporting recent common ancestry, the analysis of rate of evolution using individual gene trees indicates high substitution rates and divergence between the strains. In summary, we find that anisogamy likely evolved once in Allomyces, using convergent mechanisms to those in other taxa.peer-reviewed)

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Section: Figure 2 Evolution Of Anisogamy In Allomycesmentioning

confidence: 53%