Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production

Saikia, Rashmi Rekha; Deka, Suresh; Deka, Manab; Banat, Ibrahim M.

doi:10.1007/s13213-011-0315-5

Cited by 85 publications

(52 citation statements)

References 36 publications

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“…Biosurfactant-producing bacteria were isolated using enrichment technique described by Saikia et al (2012). Morphologically, different individual bacterial colonies were screened and streaked on Zobell marine agar (ZMA) to obtain pure cultures of the isolates.…”

Section: Isolation and Screening Of Biosurfactant-producing Bacteriamentioning

confidence: 99%

“…Morphologically, different individual bacterial colonies were screened and streaked on Zobell marine agar (ZMA) to obtain pure cultures of the isolates. Screening experiments were carried out by adding 10 m inoculum of each isolate and grown in 500 ml flasks containing 100 ml sterilized mineral salts medium (MSM) (Saikia et al, 2012) with 1% molasses as sole carbon source. The cultivations were performed in triplicate.…”

Section: Isolation and Screening Of Biosurfactant-producing Bacteriamentioning

confidence: 99%

“…Similar results have been obtained when Pseudomonas spp. grown on glycerol as sole source of carbon (Saikia et al, 2012). The ability of KVD-HR42 strain to utilize molasses as carbon source is a significant advantage as it provides an alternative use for glucose, provided that its biosurfactant production can be enhanced to economically viable values.…”

Section: Optimization Of the Production Mediamentioning

confidence: 99%

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Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Deepika¹,

Raghuram²,

Bramhachari³

2017

Afr. J. Microbiol. Res.

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Pseudomonas aeruginosa strain KVD-HR42 exhibiting growth and biosurfactant production with 1% molasses as the sole carbon source was isolated from oil contaminated mangrove sediments. Optimization of media conditions involving variations in carbon, nitrogen sources, amino acids, pH, temperature, and NaCl% were evaluated with the aim of increasing biosurfactant productivity and surface tension reduction (STR). The highest biosurfactant production of 4.83 g/L was obtained when cells were grown in mineral salts media (MSM) supplemented with 1% (w/v) molasses, NaNO 3 , and leucine 0.1% (w/v) at 35±2°C at 150 rpm after 48 h. The results obtained from kinetics study indicated that biosurfactant production, E 24 %, and rhamnose concentration were growth associated. However, maximum biosurfactant production occurred in the exponential growth phase and detected an increase in E 24 % and rhamnose concentration. The Fourier transform infrared (FTIR) spectra confirmed the rhamnolipid nature of the biosurfactant. Stability studies revealed the thermostable activity of biosurfactant (110°C for 15 min) and could also withstand wide pH and NaCl ranges. Maximum oil biodegradation of 68% was achieved with 1% waste lubricant oil (WLO). The biosurfactant emulsified various hydrocarbons with varied efficiencies. However maximum E 24 % and E 48 % activity was exhibited with n-hexadecane (69.5 and 40%). The results reveal the potential of strain KVD-HR42 biosurfactant for the bioremediation of petroleum hydrocarbons in mangrove sediments.

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Section: Isolation and Screening Of Biosurfactant-producing Bacteriamentioning

confidence: 99%

Section: Isolation and Screening Of Biosurfactant-producing Bacteriamentioning

confidence: 99%

Section: Optimization Of the Production Mediamentioning

confidence: 99%

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Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Deepika¹,

Raghuram²,

Bramhachari³

2017

Afr. J. Microbiol. Res.

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“…Present findings are in accordance with other studies; several Pseudomonas Spp. from hydrocarbon-contaminated soil had been identified (Wongsa et al 2004;Mulet et al 2011) and biosurfactant and rhamnolipid-producing native Pseudomonas has been isolated from crude-oil-contaminated soil (Saikia et al 2012;Di Martino et al 2014). However, the PHA production in these pseudomonads had not been extensively assessed.…”

Section: Isolated Bacteriamentioning

confidence: 99%

Evaluation of bioremediation potential and biopolymer production of pseudomonads isolated from petroleum hydrocarbon-contaminated areas

Goudarztalejerdi

Tabatabaei

Eskandari

et al. 2015

Int. J. Environ. Sci. Technol.

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Bacteria are diverse and abundant in soils, but only a few bacteria have known to grow on hydrocarboncontaminated areas and utilize complex carbon source such as crude oil for the synthesis of polyhydroxyalkanoate (bioremediation potential and the ability to produce important biopolymers). Among 32 samples collected from several sites of petroleum refinery soil and oily sludge of Iranian southwestern refineries, 45 oil-degrading pseudomonads were identified, and 33 % of the isolated Pseudomonas strains were able to produce polyhydroxyalkanoate using Gachsaran crude oil (2 % v/v) as carbon source. The repeated monomer composition of the copolymer produced from Gachsaran crude oil was determined by gas chromatography/mass spectrometry. The produced monomers composites contained: C 8 (3-hydroxyoctanoate), C 10 (3-hydroxydecanoate), C 12 (2-hydroxydodecanoate), C 14 (3-hydroxytetradecanoate), and C 16 (3-hydroxydecahexanoate), which are known as biopolymers. This study indicates oil-contaminated areas can be important sources for polyhydroxyalkanoate producers which can be used for the bioremediation of crude-oil-polluted sites; also polyhydroxyalkanoate has a functional role in bacterial survival and stress tolerance in the toxic environments and poor nutrient availability.

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“…Previous studies have reported the production of RL by P. aeruginosa using various carbon sources. For example, using vegetable based oils such as, soybean oil [11] and corn oil [12], glycerol [13] as well as industrial by-products or wastes [14].…”

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confidence: 99%

Application of Sweetwater as Potential Carbon Source for Rhamnolipid Production by Marine Pseudomonas aeruginosa UMTKB-5

Azemi¹,

Rashid²,

Saidin³

et al. 2016

IJBBB

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Rhamnolipid (RL), the major component of biosurfactant is commonly produced via bacterial fermentation from selected carbon sources. Biosynthesis of RL is initiated by nitrogen limitation and presence of excess carbon source. RL is completely biodegradable and non-toxic. Applications of RL include the production of fine chemicals, enhancement of biodegradation, food industries and pharmaceutical products. In this study, efforts were taken to biosynthesize RL using Pseudomonas aeruginosa UMTKB-5 isolated from marine sediment. The bacterium was fed with cane sugar refinery by-product, sweetwater as sole carbon source and 5 different types of nitrogen sources. Three different of carbon to nitrogen (C/N) ratios were tested in this study. The sweetwater was first characterized for its components. Sweetwater is mainly comprised of water (79.9 wt%) and glycerol (10.3 wt%). The total sugar content is 17.4 wt% and mainly comprises of sucrose, glucose and fructose. Biosynthesis of RL was carried out in 50 mL shaken-flask cultures, incubated at 30˚C for 72 h at 200 rpm. Sulfuric acid was used to hydrolyze rhamnose groups of RL in the culture supernatant into methyl furfural. The hydrolyzed sample containing rhamnose was reacted with orcinol (1-3-dihydroxy-5-methylbenzene). The concentration of RL produced was measured spectrometrically at 421 nm. The surface tension was measured using Du Nouy Ring method. The result obtained showed that production of RL using sweetwater was in the range of 42 -50 mg/L. The cell biomass was recorded in the range of 329 -729 mg/L. The lower surface tension (47.26 mN/m) activity occurred when ammonium chloride with C/N ratio of 35 was applied. The findings of this study demonstrate the potential application of agro-industrial by-product, sweetwater as a renewable carbon feedstock for RL production via bacterial fermentation.

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Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production

Cited by 85 publications

References 36 publications

Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Rhamnolipid biosurfactant production by Pseudomonas aeruginosa strain KVD-HR42 isolated from oil contaminated mangrove sediments

Evaluation of bioremediation potential and biopolymer production of pseudomonads isolated from petroleum hydrocarbon-contaminated areas

Application of Sweetwater as Potential Carbon Source for Rhamnolipid Production by Marine Pseudomonas aeruginosa UMTKB-5

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