Isolation of Birnavirus from Japanese Pearl Oyster &lt;i&gt;Pinctada fucata&lt;/i&gt;

Suzuki, Satoru; Kamakura, Maki; Kusuda, Riichi

doi:10.2331/fishsci.64.342

Cited by 41 publications

(44 citation statements)

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“…MABV has been isolated from various marine fish, such as Japanese flounder Paralichthys olivaceus, red sea bream Pagrus major and amberjack S. dumerili, as well as shellfish and sea squirt (Kusuda et al, 1989Suzuki et al, 1997Suzuki et al, , 1998. Such a wide host range by MABV suggests either that it may use characteristic common cellular receptors among different sea animals or that it can use multiple receptors.…”

Section: Introductionmentioning

confidence: 99%

Early interactions of marine birnavirus infection in several fish cell lines

Imajoh

Yagyu

Oshima

2003

Journal of General Virology

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Marine birnavirus (MABV), a member of the genus Aquabirnavirus, family Birnaviridae, is an unenveloped icosahedral virus with two genomes of double-stranded RNA. The mechanisms of MABV adsorption and penetration are still undetermined. This work examined MABV infection in susceptible and resistant fish cell lines. MABV adsorbed not only onto the cell surfaces of susceptible (CHSE-214 and RSBK-2) cells but also onto resistant (FHM and EPC) cells. Furthermore, the virus entered the cytoplasm through the endocytotic pathway in CHSE-214, RSBK-2 and FHM cells but did not penetrate EPC cells. Thus, restriction of the MABV replication cycle is different between resistant FHM and EPC cells. The virus was found to bind to an around 250 kDa protein on CHSE-214, RSBK-2, FHM and EPC cells. Thus, this 250 kDa protein may be a major MABV receptor that exists in the plasma membranes of all four cell lines examined. This result suggests further that another receptor for virus penetration may exist in CHSE-214, RSBK-2 and FHM cells but not in EPC cells.

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Section: Introductionmentioning

confidence: 99%

Early interactions of marine birnavirus infection in several fish cell lines

Imajoh

Yagyu

Oshima

2003

Journal of General Virology

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“…We have isolated MABV from Agemaki (jack knife clam), Sinonovacula consticta (Suzuki et al, 1997 a) and the Japanese pearl oyster, Pinctada fucata (Suzuki et al, 1998a), although the pathogenicity of the virus in the experimental infections appeared weak (Suzuki et al, 1997a;1998b). The strains of MABV isolated from shellfish appear to be similar to strains from fish based on serological and genomic properties (Suzuki et al, 1997a;1998a).…”

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“…The strains of MABV isolated from shellfish appear to be similar to strains from fish based on serological and genomic properties (Suzuki et al, 1997a;1998a).…”

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Detection of a Marine Birnavirus in Wild Molluscan Shellfish Species from Japan.

Nojima

1999

Fish Pathol.

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ABSTRACT--Thirteen wild molluscan shellfish species collected from 8 Prefectures in Japan were surveyed for the presence of marine birnavirus (MABV) by the polymerase chain reaction (PCR) technique and culture method. Approximately 60% of bivalves and 35% of gastropods tested had detectable MABV genome, although the prevalence of positive specimens varied among species.The PCR-positive shellfish were submitted to virus isolation. The isolation rate was low, suggesting that the MABV was in a state of persistent infection in these shellfish. Seventy four % of the examined virus strains were different in a position of the nucleotide sequences of the PCR products from that of MABV strains obtained from fish.

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“…The varying pathogenicity found in this study might have been influenced by differences in size or age of the fish together with the species specificity. Environmental stressors can alter the pathogenicity of MABV in molluskan shellfish (Chou et al 1994, Suzuki et al 1998b). Stressors can be disregarded in this study because the experimental conditions in all groups were the same, except for water temperature, which was adjusted to the optimum range for each species.…”

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Infectivity of aquabirnavirus strains to various marine fish species

Isshiki¹,

Nagano²

2001

Dis. Aquat. Org.

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To determine the infectivity of marine birnavirus (MABV) in various marine fish species, experimental infection was performed in combination groups of 5 fish species with 7 strains of MABV and 1 strain of infectious pancreatic necrosis virus (IPNV). Mortality was observed in yellowtail Seriola quinqueradiata and amberjack S. dumerili infected with MABV strains Y-6, Y-10K and H-1, but not in other infected species. MABV was reisolated from most combination groups, but the virus isolation rate and virus infectivity titer were often significantly different among groups with the same fish species or with the same virus strain. All MABV strains replicated well in makogarei Limanda yokohamae, but only slightly in tiger puffer Takifugu rubripes. IPNV also replicated in all fish species without causing death. The isolation rate and infectivity titer of IPNV were similar to or higher than those of non-virulent strains of MABV. In conclusion, the infectivity of MABV for different fish species is considered to change, which is an important factor in the development of the infection cycle of this virus among marine organisms. KEY WORDS: Aquabirnavirus · Marine birnavirus · Infectious pancreatic necrosis virus · Marine fish · Infectivity · Experimental infection Resale or republication not permitted without written consent of the publisherDis Aquat Org 46: [109][110][111][112][113][114] 2001 We report the infectivity of several aquabirnavirus strains, including MABV and IPNV, against some marine fish species. The mortality, virus isolation rate and infectivity titer were compared among experimental groups. MATERIALS AND METHODSCell line. The CHSE-214 cell line (chinook salmon embryo; Lannan et al. 1984) was used in this study. This cell line was grown at 20°C in Eagle's minimum essential medium (Nissui, Japan) supplemented with 10% fetal bovine serum.Virus strains. The aquabirnavirus strains used in this study are shown in Table 1. Seven strains of MABV and 1 strain of IPNV isolated from fish and shellfish cultured in various areas of western Japan during natural outbreaks were examined. Among them, an unpublished strain, KMKBV-9701, was isolated from hatchery-reared makogarei Limanda yokohamae juveniles (27 d old, average 10 mm in total length) collected during mass mortalities at a water temperature of 15°C in February 1997 in Kagawa Prefecture. Each strain was grouped into MABV or IPNV by genogrouping based on amino acid sequences in the VP2/NS junction region (Hosono et al. 1996). All these strains were propagated on CHSE-214 cells at 20°C. The susceptibility of the CHSE-214 cell line at 20°C was nearly equal to all the used virus strains (data not shown).Experimental fish. Five species of marine fish were examined: yellowtail (mean body weight 2.2 g), amberjack Seriola dumerili (17.7 g), black sea bream, Acanthopagrus schlegeli (6.6 g), tiger puffer (3.0 g) and makogarei (6.2 g). These fishes were produced at Yashima Station of Japan Sea-Farming Association, Kagawa Prefectural Sea-Farming Center, or a priva...

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Isolation of Birnavirus from Japanese Pearl Oyster <i>Pinctada fucata</i>

Cited by 41 publications

References 1 publication

Early interactions of marine birnavirus infection in several fish cell lines

Early interactions of marine birnavirus infection in several fish cell lines

Detection of a Marine Birnavirus in Wild Molluscan Shellfish Species from Japan.

Infectivity of aquabirnavirus strains to various marine fish species

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