Isolation of Brucella melitensis strains from Syrian bovine milk samples

Al-Mariri, Ayman

doi:10.15547/bjvm.842

Cited by 11 publications

(8 citation statements)

References 49 publications

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“…Meanwhile, ELISA and PCR approaches are expensive and unavailable in many developing countries. However, a recent Syrian study has found that PCR and culture approach yielded the same results while the MRT showed lower rates of positive results (48).…”

Section: Discussionmentioning

confidence: 97%

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The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

Almashhadany¹

2019

IJVS

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This study was undertaken to monitor Brucella antibodies in the milk of cows and buffaloes in Erbil Governorate, Kurdistan Region, Iraq, using mik ring test (MRT) assay. A total of 210 samples of milk (130 from cows and 80 from buffaloes) were randomly collected from lactating females. The overall prevalence of Brucella antibodies in all the milk samples was 8.6% (18/210). The highest rate was 9.2% found in the cow milk (12/130), while the lowest rate was 7.5% of the buffalo's milk (6/80). Out of 210 milk samples, only 15 (7.1%) were culture-positive for Brucella; about 7.7% (10/130) and 6.3% (5/80) from cows and buffaloes respectively. In terms of comparison between MRT and standard milk culture method, MRT was found more sensitive (83%), specific (98%), with the accuracy of 97% in comparison to the employed culture approach to detect Brucellosis agents in milk. The results also revealed that 70% and 60% of isolates were Brucella abortus, while 30% and 40% were Brucella melitensis from the milk of cow and buffaloes respectively. The highest rate of frequency for Brucella antibodies according to MRT was found in February (12.1%), while the lowest rate was found in June (5.7%). This study emphasizes that Brucellosis is still a significant public health hazard in the Kurdistan region. The study recommends MRT adoption in routine monitoring of brucellosis in milk collection centre, dairy factories, and farm. Consumers are also recommended to sufficiently heat the milk to destroy this foodborne pathogen before consumption or industrial processing.

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Section: Discussionmentioning

confidence: 97%

“…In Syria, a recent study isolated Br. melitensis from bovine raw milk samples at a rate of 25% (48). Furthermore, in San Paulo, 30% of bovine screened milk samples yielded Br.…”

Section: Discussionmentioning

confidence: 97%

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

Almashhadany¹

2019

IJVS

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“…MRT has been effectively used in several regions such as Chile, Sudan, India, Iraq, and Pakistan (Rivera et al, 2002;Abdalla and Hamid, 2012;Ali et al, 2013;Mohamand et al, 2014;Almashhadany, 2019). Furthermore, performance of MRT was found to be better than for other serological tests (Al-Mariri, 2015;Al-mashhadany, 2018 b ). The accuracy of MRT in ruling out the infection has also been reported previously (Al-mashhadany, 2018 b ;Al-Shemmari, 2018;Almashhadany, 2019).…”

Section: Discussionmentioning

confidence: 88%

Serological and Bacteriological Screening of Brucellosis in Blood and Milk of Cows and Buffaloes

Almashhadany¹

2021

SVU-International Journal of Veterinary Sciences

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Brucellosis remains a serious infection to human and animal populations in developing countries with detrimental effects on public health. The study aimed to evaluate the occurrence of brucellosis in cow and buffaloes at Erbil Governorate, Iraq by detection anti-Brucella antibodies and isolation of Brucella species. A total of 265 blood samples (140 from cows and 125 from buffaloes) were collected from villages around Erbil city, and 320 raw milk samples (170 from cow and 150 from buffaloes) were randomly collected from dairy farms during the period from July to December 2019. The results showed an overall seroprevalence of 11.7% according to rose Bengal test (RBT). The isolation of Brucella abortus was 7/19 (36.8%) and 12 / 19 (63.2%), while 9/24 (37.5%) and 15 /24 (62.5%) were Brucella melitensis from cattle and buffaloes milk samples, respectively. Noticeable increase in occurrence was found in November (20.8%), while the lowest rate was seen in July (5.9%). In conclusion, brucellosis is still a significant public health hazard in Erbil Governorate. Based on the test performance, the study recommends MRT as a rapid screening test for detecting brucellosis in milk in farms, centers, and dairy factories. Consumers are also recommended to adequately pasteurize the milk in order to kill this milk-borne pathogen before consumption.

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“…All of the isolates were molecularly confirmed as the genus and further characterized as B. abortus and B. melitensis. Some other internal (33,34) and external (35,36) researches have also been documented the identification of zoonotic Brucella spp. in ruminants' milk samples using conventional bacteriological method and molecular confirmation of the isolates using PCR.…”

Section: Discussionmentioning

confidence: 99%

“…in ruminants' milk samples using conventional bacteriological method and molecular confirmation of the isolates using PCR. The reported contamination rate was from 1.28% to 25% (33)(34)(35)(36).…”

Section: Discussionmentioning

confidence: 99%

Contamination of Bovine Milk With Brucella spp.: A Current Public Health Menace in Kurdistan Province of Iran

Asgari

Ahmadi

2021

Avicenna J Clin Microbiol Infect

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Background: Brucellosis, a deteriorating zoonotic disease, is very common in most parts of Iran. Consumption of contaminated milk and dairy products is one of the most significant ways for transmission of the infections to human. Since the close rearing of cattle and sheep is practiced in Kurdistan province of Iran, the infection of cow with non-specific species is not out of mind. The present study aimed to determine the frequency of bovine milk contamination with zoonotic Brucella spp. Methods: A total of 240 milk samples, equally from traditional and industrialized dairy farms, were collected aseptically. Conventional microbiological method was used for isolation of the bacterium, followed by the genotypic identification of the isolates. Moreover, direct molecular processing of the samples was carried out for detection of the bacterial genome. The positive samples were further genotypically assessed to identify the contamination as Brucella abortus or Brucella melitensis. Results: In general, 16 (6.66%) and 15 (6.25%) of the samples were contaminated with Brucella spp. in phenotypic and genotypic methods, respectively. The proportion of contamination with B. abortus and B. melitensis in phenotypic and genotypic methods were 5% and 1.66%, and 5% and 1.25%, respectively. The overall rate of contamination in traditional milk samples was more than industrialized samples. Conclusions: Contamination of bovine milk with Brucella spp. is a serious threat to public health in the studied region. Continuous vaccination, application of test and slaughter policy, and presumption of pasteurized milk and dairy products are highly recommended.

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Isolation of Brucella melitensis strains from Syrian bovine milk samples

Cited by 11 publications

References 49 publications

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

Serological and Bacteriological Screening of Brucellosis in Blood and Milk of Cows and Buffaloes

Contamination of Bovine Milk With Brucella spp.: A Current Public Health Menace in Kurdistan Province of Iran

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