Isolation of Candidatus Bartonella melophagi from Human Blood1<sup/>

Maggi, Ricardo G.; Kosoy, Michael; Mintzer, Melanie; Breitschwerdt, Edward B.

doi:10.3201/eid1501.081080

Cited by 95 publications

(74 citation statements)

References 15 publications

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“…Bartonella spp. enfeksiyonları arasında kedi tırmığı hastalığı, basiller anjiomatozis, basiller peliozis, Carrion hastalığı, enfektif endokardit ve özellikle insan immünyetmezlik virüsü (HIV) pozitif olgularda nörolojik sendromlar sayılabilir 3,5 . Ülkemizde Bartonella'lara bağlı kedi tırmığı hastalığı ve basiller anjiomatozis olgu sunuları bulunmaktadır [6][7][8][9] .…”

Section: Introductionunclassified

Seroprevalence of Bartonella henselae and Bartonella quintana in Blood Donors in Aydin Province, Turkey

Aydın¹,

Bülbül²,

Telli³

et al. 2014

Mikrobiyol Bul

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ÖZETBartonella türleri insanlarda kedi tırmığı hastalığı, basiller anjiomatozis, basiller peliozis, Carrion hastalığı, enfektif endokardit ve siper ateşi gibi pek çok hastalığa yol açmaktadır. Türkiye'de Bartonella'lara bağlı kedi tırmığı hastalığı ve basiller anjiomatozis olgu sunumları bulunmaktadır. Aydın merkez ve ilçele-rinde yapılan çalışmalarda, risk gruplarında Bartonella henselae seropozitifliği ortalama %11.5; evde kedi/ köpek besleyenlerde ise ortalama %26.5'e kadar çıkabilmektedir. Kesitsel epidemiyolojik bir araştırma olarak planlanan bu çalışmada, Aydın'da kan donörlerinde B.henselae ve Bartonella quintana seroprevalansının ve transfüzyon riski açısından öneminin belirlenmesi amaçlanmıştır. Çalışmaya, 2011 yılı Ocak ayında hastanemizin kan merkezine başvuran sıralı 333 kan donörüne (49 kadın, 284 erkek) ait serum örnekleri dahil edilmiştir. Örneklerde B.henselae ve B.quintana IgG antikorları, iki farklı ticari kit (Vircell, İspanya; Focus, ABD) kullanılarak indirekt floresan antikor yöntemiyle araştırılmıştır. Çalışılan örnekler iki ayrı araştırmacı tarafından floresans mikroskop ile x400 büyütme ile incelenerek floresans 1+ ile 4+ arasında derecelendirilmiş, 2+ ve üzerinde olanlar pozitif olarak değerlendirilmiştir. Kan donörlerinin %3.3 (11/333)'ünde B.henselae IgG seropozitifliği saptanmıştır. Seropozitiflik, kadın donörlerde %4.1, erkek donörlerde ise %3.2 olarak bulunmuş; cinsiyetler arasında istatistiksel olarak anlamlı bir fark saptanmamıştır (p= 0.668). B.henselae IgG titreleri; 6 (%1.8) örnekte 1/64, 4 (%1.2) örnekte 1/128, 1 (%0.3) örnekte ise 1/1024 olarak izlenmiştir. B.henselae IgG pozitif örneklerde daha düşük titrede B.quintana IgG pozitifliği tespit edilmiş ve çapraz pozitiflik olarak değerlendirilmiştir. Kullanılan kitlere göre floresans dereceleri karşılaştırıldığında, bir titre dışında uyumlu sonuçlar elde edilmiştir. İki araştırmacının değerlendirme sonuçları karşılaştırıldığında ise, 1+ ve altında olan derecelendirmelerde farklar olduğu belirlenmiştir. Araştırıcılar arasındaki ve kitlere bağlı bu değerlendirme farkları araştırmanın sonuçlarını etkilememiştir. Sonuç olarak, Aydın'da kan donörlerinde B.henselae enfeksiyonu bulunabileceği ve donör-lerin detaylı sorgulanmasının transfüzyon yoluyla bulaşı önlemede yardımcı olabileceği düşünülmüştür.

show abstract

Section: Introductionunclassified

Seroprevalence of Bartonella henselae and Bartonella quintana in Blood Donors in Aydin Province, Turkey

Aydın¹,

Bülbül²,

Telli³

et al. 2014

Mikrobiyol Bul

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“…While Dehio and coauthors (9) suggested that B. schoenbuchensis transmitted with the bites of deer ked may establish a local infection in the skin and thereby contribute to the etiology of deer ked dermatitis, there is limited evidence to support a role for the ruminant-infecting Bartonella, such as B. schoenbuchensis, B. capreoli, B. melophagi, or B. chomelii, as zoonotic agents. Recently, Maggi and coauthors (42) reported isolation of the closely related "Candidatus Bartonella melophagi" from the blood of two diseased women, but no causal relationship between the disease and the infection was proven.…”

Section: Figmentioning

confidence: 99%

Bartonella Infections in Deer Keds (Lipoptena cervi) and Moose (Alces alces) in Norway

Duodu

Madslien

Hjelm

et al. 2013

Appl Environ Microbiol

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cInfections with Bartonella spp. have been recognized as emerging zoonotic diseases in humans. Large knowledge gaps exist, however, relating to reservoirs, vectors, and transmission of these bacteria. We describe identification by culture, PCR, and housekeeping gene sequencing of Bartonella spp. in fed, wingless deer keds (Lipoptena cervi), deer ked pupae, and blood samples collected from moose, Alces alces, sampled within the deer ked distribution range in Norway. Direct sequencing from moose blood sampled in a deer ked-free area also indicated Bartonella infection but at a much lower prevalence. The sequencing data suggested the presence of mixed infections involving two species of Bartonella within the deer ked range, while moose outside the range appeared to be infected with a single species. Bartonella were not detected or cultured from unfed winged deer keds. The results may indicate that long-term bacteremia in the moose represents a reservoir of infection and that L. cervi acts as a vector for the spread of infection of Bartonella spp. Further research is needed to evaluate the role of L. cervi in the transmission of Bartonella to animals and humans and the possible pathogenicity of these bacteria for humans and animals.

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“…B. melophagi has been isolated from various lots of commercial defibrinated sheep blood in the United States (20). Interestingly, this species has also been isolated from the blood of two female patients, and it has been described as a new human pathogen (21). Although a high prevalence of this pathogen has been found in the sheep ked Melophagus ovinus (22,23), which is the potential vector of this Bartonella species, others studies have failed to identify Bartonella infection in sheep (16,22).…”

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confidence: 99%

Bartonella chomelii Is the Most Frequent Species Infecting Cattle Grazing in Communal Mountain Pastures in Spain

Antequera-Gómez

Lozano-Almendral

Barandika

et al. 2015

Appl Environ Microbiol

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The presence of Bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR/RLB), whereas sheep (n ‫؍‬ 133) or horses (n ‫؍‬ 91) were found not to be infected by this pathogen. Bartonella infection was significantly associated with age, since older animals showed a higher prevalence than heifers and calves. In contrast to other studies, B. chomelii was the most frequent species found in cattle (14/17), while B. bovis was detected in only three animals. Moreover, 18 B. chomelii isolates and one B. bovis isolate were obtained from nine animals. Afterwards, B. chomelii isolates were characterized by a multilocus sequence typing (MLST) method which was adapted in this study. This method presented a high discrimination power, identifying nine different sequence types (STs). This characterization also showed the presence of different STs simultaneously in the same host and that STs had switched over time in one of the animals. In addition, B. chomelii STs seem to group phylogenetically in two different lineages. The only B. bovis isolate was characterized with a previously described MLST method. This isolate corresponded to a new ST which is located in lineage I, where the B. bovis strains infecting Bos taurus subsp. taurus are grouped. Further studies on the dynamics of Bartonella infection in cattle and the potential ectoparasites involved in the transmission of this microorganism should be performed, improving knowledge about the interaction of Bartonella spp. and domestic ungulates.

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Isolation of Candidatus Bartonella melophagi from Human Blood1

Cited by 95 publications

References 15 publications

Seroprevalence of Bartonella henselae and Bartonella quintana in Blood Donors in Aydin Province, Turkey

Seroprevalence of Bartonella henselae and Bartonella quintana in Blood Donors in Aydin Province, Turkey

Bartonella Infections in Deer Keds (Lipoptena cervi) and Moose (Alces alces) in Norway

Bartonella chomelii Is the Most Frequent Species Infecting Cattle Grazing in Communal Mountain Pastures in Spain

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