Isolation of carotenoids from plant materials and dietary supplements by high-speed counter-current chromatography

Aman, Robert; Carle, Reinhold; Conrad, Jürgen; Beifuß, Uwe; Schieber, Andreas

doi:10.1016/j.chroma.2005.03.055

Cited by 83 publications

(38 citation statements)

References 16 publications

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“…Artifact formation is also eliminated, since it used no solid support. HSCCC has been used for analysis, separation and purification of bioactive compounds from natural products, such as alkaloids, hydroxyanthraquinones, flavonoids and saponins (Shibusawa et al, 2000;Liu et al, 2004;Aman et al, 2005;Chu et al, 2005;Li and Chen, 2005;Zhou et al, 2006;Gutzeit et al, 2007;Yao et al, 2007). This technique presents maximum capacity with an excellent sample recovery and high efficiency, and can be employed for preparative-scale separations in a straightforward manner (Tong et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Assimopoulou

Sturm

Stuppner

et al. 2008

Biomedical Chromatography

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Alkannin and shikonin (A/S) and their derivatives have been found in the roots of several Boraginaceous species and are also produced through plant tissue cultures. The chiral compounds A/S are potent pharmaceutical substances with a wide spectrum of biological and pharmacological activities like wound healing, antimicrobial, anti-inflammatory, anticancer and antioxidant activity. High-speed counter-current chromatography (HSCCC) was applied for the first time to the separation, preparative isolation and purification of A/S and their esters from extracts of Alkanna tinctoria roots, as well as commercial samples. The constituents of HSCCC fractions and their purity were determined by high-performance liquid chromatographydiode array detection-mass spectrometry (HPLC-DAD-MS), since DAD cannot detect oligomeric A/S derivatives that are present in most of the samples containing the respective monomeric derivatives. The purity of HSCCC fractions was compared with the one of fractions isolated by column chromatography (CC) using as stationary phases silica gel and Sephadex LH-20. As shown, the purity of monomeric alkannin/shikonin was greater by HSCCC than CC separation of commercial A/S samples.

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Section: Introductionmentioning

confidence: 99%

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Assimopoulou

Sturm

Stuppner

et al. 2008

Biomedical Chromatography

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“…Levels of allophycocyanin (APC), phycocyanin (PC), and phycoerythrin (PE) were determined by spectrophotometry (Hitachi, Model 100-20; Hitachi Co., Japan) at 498, 615 and 651 nm, and concentrations were calculated using the equations of Kursar & Alberte (1983). The extraction of carotenoids was performed according to Aman et al (2005). Carotenoids were extracted from samples (1.0 g FW, n=4) using methanol 80 %.…”

Section: Photosynthetic Pigmentsmentioning

confidence: 99%

Effects of salinity on the physiology of the red macroalga, Acanthophora spicifera (Rhodophyta, Ceramiales)

et al. 2017

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Salinity is an important abiotic factor since it is responsible for the local and/or regional distribution of algae. In coastal regions, salinity changes with prevailing winds, precipitation and tide, and particularly in extreme intertidal conditions. Acanthophora spicifera is a red seaweed that occurs in the supratidal region in which changes in abiotic conditions occur frequently. Th is study evaluated the eff ects of salinity on the metabolism and morphology of A. spicifera. Algae were acclimatized under culture conditions with sterilized seawater for seven days. Experiments used diff erent salinities (15 to 50 psu) for seven days, followed by metabolic analyses. Th is study demonstrates that extreme salinities aff ect physiological parameters of A. spicifera, such as decrease in growth rate, as well as morphological parameters and concentrations of secondary metabolites. Acanthophora spicifera exhibited high tolerance to 25 to 40 psu, with little change in physiology, which favors the occurrence of this species in diverse environments. However, 15, 20, 45 and 50 psu were the most damaging and led to loss of biomass, depigmentation of apices, and the highest concentrations of antioxidant metabolites. Th e 50 psu treatment caused the greatest changes in general, greatly reducing a biomass and chlorophyll content, and facilitating the presence of endophytes.

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“…The other part of the stored leaves green mass was used for the assessment of carotenoid content in accordance with the procedure proposed by AMAN et al (2005). For that, 375 mg of plant tissue was macerated in liquid N, to which 15 mL of Hexane:Acetone (1:1 -v/v), containing 100 mg L -1 of BHT (butylate dhydroxytoluene) was added.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, higher chlorophyll content can promote a larger antioxidant, antimutagenic and chemopreventive activity in humans (LANFER-MARQUEZ, 2003), inducing cancer prevention (SIMONICH et al, 2007;MCQUISTAN et al, 2012) and contributing for healing process (TANAKA et al, 1997). Carotenoids are vitamin A precursors and also powerful antioxidants, working in the prevention of degenerative diseases (RODRIGUEZ-AMAYA, 2001;AMAN et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Single-head broccoli response to nitrogen application

Ambrosini¹,

Voges²,

Benevenuto³

et al. 2015

Científica

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Information regarding nitrogenous fertilization in broccoli for Santa Catarina is still scarce. Therefore, this study aimed at analyzing the nitrogen effect upon the levels of chlorophyll (a, b and total), carotenoids and nitrogen (N) forms in leaves, and upon the yield of inflorescence, stem and leaves of singlehead broccoli. The experiment was conducted in September, 2011, in Florianópolis (SC), with seedlings of single-head broccoli (BRO 68 -Syngenta), in completely randomized blocks, with five treatments (0, 75, 150, 200 and 250 kg ha -1 of N, in the form of urea) and three replications. In the blooming, the fourth leaf from the ground was harvested from each broccoli for the following analyses in triplicate: chlorophyll levels (a, b and total), carotenoids and N forms (NH 4 + , NO 3 -and mineral N). At the end of the cycle, the yield of inflorescence, body (leaves + stem) and total were estimate. The levels of N lead to increased levels of chlorophyll a, b and total, as well as the N forms in leaves and the yield of inflorescence, leaves, and stem of single-head broccoli. The increased N doses promoted increment of carotenoid levels on leaves until 170 kg ha -1 of N, having a decrease for superior doses. The N forms in the plant had been incremented with increasing of N doses supply, reflecting in increased yield of broccoli. The N application in the soil stimulated the synthesis of chlorophylls and carotenoids on leaves, promoting better nutritional quality.Additional keywords: Brassica oleracea L. var. italica Plenck; carotenoids; chlorophyll; nitrogenous fertilization; yield. ResumoAs informações sobre adubação nitrogenada em brócolis para Santa Catarina (SC) ainda são escassas. Por isso, objetivou-se avaliar o efeito do nitrogênio (N) sobre os teores de clorofila (a, b e total), carotenoides e formas de N nas folhas, e sobre a produtividade de inflorescência, talo e folhas de brócolis cabeça única. O experimento foi implantado em setembro de 2011 em Florianópolis (SC), com mudas de brócolis cabeça única (BRO 68 -Syngenta), em delineamento de blocos completos casualizados, com cinco tratamentos (0, 75, 150, 200 e 250 kg ha -1 de N, na forma de ureia) e três repetições.No pleno florescimento das plantas, foi coletada a quarta folha a partir do solo para as seguintes avaliações, em triplicata: teores de clorofila (a, b e total), carotenoides e formas de N (NO 3 -, NH 4 + e N mineral). No final do ciclo, foi estimada a produtividade de inflorescência, corpo (folhas + talo) e total. As doses de N incrementaram os teores de clorofila a, b e total, as formas de N na folha e a produtividade de inflorescência, talo e folhas de brócolis cabeça única. O aumento das doses de N proporcionou o incremento do teor de carotenoides na folha até 170 kg ha -1 de N, decrescendo nas doses superiores. O aumento das doses de N incrementou as formas desse nutriente na planta, refletindo em aumento da produtividade de brócolis. A aplicação de nitrogênio no solo estimulou a síntese de clorofilas e carotenoides nas folha...

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Isolation of carotenoids from plant materials and dietary supplements by high-speed counter-current chromatography

Cited by 83 publications

References 16 publications

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high‐speed counter‐current chromatography

Effects of salinity on the physiology of the red macroalga, Acanthophora spicifera (Rhodophyta, Ceramiales)

Single-head broccoli response to nitrogen application

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