Isolation of catecholamines in biological fluids by boric acid gel

Higa, Sadayoshi; Suzuki, Tomokazu; Hayashi, Akira; Tsuge, Isao; Yamamura, Yûichi

doi:10.1016/0003-2697(77)90285-8

Cited by 122 publications

(27 citation statements)

References 7 publications

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“…Tetraborate buffer was originally selected because it forms a reversible complex with catechol groups [9, 32] to prevent oxidation under high pH conditions. Our present work first focused on modifying reaction conditions to improve sensitivity and reduce the number of steps.…”

Section: Resultsmentioning

confidence: 99%

Benzoyl chloride derivatization with liquid chromatography–mass spectrometry for targeted metabolomics of neurochemicals in biological samples

Wong

Malec

Mabrouk

et al. 2016

Journal of Chromatography A

216

191

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Widely targeted metabolomic assays are useful because they provide quantitative data on large groups of related compounds. We report a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method that utilizes benzoyl chloride labeling for 70 neurologically relevant compounds, including catecholamines, indoleamines, amino acids, polyamines, trace amines, antioxidants, energy compounds, and their metabolites. The method includes neurotransmitters and metabolites found in both vertebrates and insects. This method was applied to analyze microdialysate from rats, human cerebrospinal fluid, human serum, fly tissue homogenate, and fly hemolymph, demonstrating its broad versatility for multiple physiological contexts and model systems. Limits of detection for most assayed compounds were below 10 nM, relative standard deviations were below 10%, and carryover was less than 5% for 70 compounds separated in 20 min, with a total analysis time of 33 min. This broadly applicable method provides robust monitoring of multiple analytes, utilizes small sample sizes, and can be applied to diverse matrices. The assay will be of value for evaluating normal physiological changes in metabolism in neurochemical systems. The results demonstrate the utility of benzoyl chloride labeling with HPLC-MS/MS for widely targeted metabolomics assays.

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Section: Resultsmentioning

confidence: 99%

Benzoyl chloride derivatization with liquid chromatography–mass spectrometry for targeted metabolomics of neurochemicals in biological samples

Wong

Malec

Mabrouk

et al. 2016

Journal of Chromatography A

216

191

View full text Add to dashboard Cite

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“…74^77 These gels di¡er mainly in the Column switching using TOSOH automatic catecholamine Eurogenetics UK Ltd, Teddington reverse-phase and ionanalyser with uorimetric detection UK exchange columns system spacing of the boric acid from the support matrix and are believed to retain the catecholamines by forming a complex with the vicinal hydroxyl groups at increased pH. 77,78 Elution is readily achieved by decreasing the pH, providing a simple and rapid procedure prior to HPLC analysis. However, one disadvantage previously noted was the variation in the properties of di¡erent batches of the gels.…”

Section: Hplc With Boric Acid Isolationmentioning

confidence: 99%

Measurement of catecholamines and their metabolites

2004

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Analysis of the low levels of catecholamines and metabolites in tissue and biological uids has necessitated the use of highly sensitive analytical techniques. Earlier procedures utilizing radioenzymatic and immunological assays, gas chromatography or uorimetry have generally been superseded by highly sensitive and selective chromatographic methods utilizing electrochemical or uorimetric detection. The development of high-performance liquid chromatography (HPLC) methods for the measurement of plasma metadrenalines and the combination of HPLC with tandem mass spectrometry provides additional procedures with minimum interference from drugs and drug metabolites. This review summarizes the methodology currently available for the measurement of catecholamines and metabolites in plasma and urine, the in uence of sample collection protocols and the clinical application of the methods for the biochemical detection of catecholamine-secreting tumours.

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“…Since the occurrence of nucleosides in physiological fluids turned out to be predicative for the diagnosis of pathobiochemical processes, the on-line System is the method of choice, äs it is particularly suitable for automatization and applicable for routine clinical analysis. As affinity chromatography on boronic acid-functionalized polymers is also well known for the pre-purification of catecholamines in physiological fluids (34)(35)(36)(37)(38), for the Separation of ribonucleosides and ribonucleotides from the corresponding deoxy-compounds (22,39,40), the Separation of RNA from DNA (41,42) and the Separation of queuine-containing tRNA from non-queuine tRNA (41) or aminoacyl-tRNA from uncharged tRNA (41,42) the use of boronate HPLAC in combination with the presented column switching technique offers a wide ränge of application.…”

Section: Discussionmentioning

confidence: 99%

Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

Hagemeier¹,

Kemper²,

Ks³

et al. 1984

Clinical Chemistry and Laboratory Medicine

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The authors wish to congratulate Professor Walther Lamprecht, Hannover, on the occasion of his sixtieth birthday Summary:We describe an on-line multi-cplumn high performance liquid Chromatographie method for the selective clean-up and analysis of major and minor ribonucleosides in physiological fluids. Quantitative data obtained for the determination of some methylated ribonucleosides in human urines are compared with those obtained with the traditional off-line method.The on-line technique developed in our laboratory is distinguished from the off-line method by the following features:(1) Sample clean-up and analysis of the target-compounds can easily be automatized, (2) Total time of analysis, for example of urinary ribonucleosides, is decreased to 35 minutes, (3) Laborious and error-prone evaporation and redissolution Steps are avoided, (4) Reliability of the overall analytical System can be controlled with ease, (5) Small sample-volumes can be applied directly, (6) Sensitive samples can be processed very rapidly under mild conditions, (7) Results obtained with the -line and off-line-techniques compare well. Hagemeier, Kemper, Boos and Schlimme: Quantitative determination of minor ribonucleosides (5) Kleine Probenvolumina können direkt appliziert werden, (6) Empfindliche Proben können in kürzester Zeit unter schonenden Bedingungen analysiert werden, (7) Die mit der "on-line" wie mit der "off-line"-Technik erhaltenen Ergebnisse zeigen eine gute ÜbereinŜ timmung. Entwicklung einer chromatographischen Methode zur quantitativen Bestimmung seltener Ribonucleoside in physiologischen Flüssigkeiten Charakterisierung und quantitative Bestimmung von seltenen Ribonucleosiden in physiologischen Flüssig

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Isolation of catecholamines in biological fluids by boric acid gel

Cited by 122 publications

References 7 publications

Benzoyl chloride derivatization with liquid chromatography–mass spectrometry for targeted metabolomics of neurochemicals in biological samples

Benzoyl chloride derivatization with liquid chromatography–mass spectrometry for targeted metabolomics of neurochemicals in biological samples

Measurement of catecholamines and their metabolites

Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

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