Isolation of chick primordial germ cells from stages 4–8 embryos

Matsumura, George; England, Marjorie A.

doi:10.1002/ar.1092350413

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…On collagen IV, the morphology of the cumulus cells more closely resembled the control cells after 2-4 h. They were more rounded and upright on the surface but lamellipodia were present around the bases of the cells. It may be that collagen IV induces cells to move in a similar manner to that described for PGCs (Matsumura & England, 1993). Larger areas of substrate were observed between neighbouring cells and there was frequently a large amount of ECM visible between and on the surfaces of the cells.…”

Section: Cell Morphology On a Collagen-coated Substratementioning

confidence: 72%

Human cumulus cell complexes studied in vitro by light microscopy and scanning electron microscopy

Kennedy

England

Mills

1994

Zygote

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SummaryVarious researchers describe the morphology of cumulus cells (CC) in vitro, but few have investigated their behaviour on plastic. Knowledge concerning the behaviour of human CC could be useful in improving the success of in vitro fertilisation procedures. This study aimed to describe the morphology and behaviour of CC in vitro and to investigate movement on a collagen-coated substrate. Following collection some cumulus were mechanically dissected from those surrounding the oocyte. Cumulus aggregates were cultured over 24 h using Earle's medium supplemented with 8% albumin. Substrata were plastic coverslips coated with collagens I, IV, or mixed collagens. Cumulus cultured over corresponding time periods on uncoated coverslips served as controls. Specimens were fixed and prepared for scanning electron microscopy. Over 24 h the controls began exhibiting the morphological features associated with cell movement: cell surface protrusions changed from blebs to microridges, lamellipodia and leading lamellae; cell shape altered from rounded and upright, to flattened. Extracellularmatrix (ECM) transformed from a thick, sheet-like substance to a thin, fibrous material. By 24 h, cells contacting ECM remained rounded showing few features of movement. Collagens enhanced attachment of CC as a monolayer on the substrate. Cell morphology varied according to the collagen type used. On mixed collagens, cells attached rapidly, appearing to be predominantly non-motile. On collagen type I there was less attachment of cells but increased motility. On collagen type IV there was decreased attachment and the cells remained spherical. In conclusion, collagens enhance the settling of cumulus cells on a plastic substrate and the cells exhibit some specificity in attaching to collagens.

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Section: Cell Morphology On a Collagen-coated Substratementioning

confidence: 72%

Human cumulus cell complexes studied in vitro by light microscopy and scanning electron microscopy

Kennedy

England

Mills

1994

Zygote

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“…Mesoblast cells and healing hypoblast, but probably also dissociated hypoblast cells, are unable to move on the fibrillar band (Andries et ai, 1985;Harrisson et al, 1992), while that primordial germ cells use the fibrils as a contact guidance system for migration (England, 1983, for review). Although both primordial germ cells and mesoblast cells are highly motile cells, the former cells during early and late development are characterized by a rounded shape and a low degree of spreading in vivo and in vitro (Lee et al, 1978;Kuwana and Fujimoto, 1984;Matsumura and England, 1993), whereas the latter cells are flat and polarized, with a high capability of spreading and a relatively low adhesiveness (Sanders, 1980).…”

Section: Discussionmentioning

confidence: 99%

Behaviour of dissociated hypoblast cells on the basal lamina and on extracellular fibrils in the gastrulating chicken embryo.

Andries

Harrisson

Vakaet

1994

Cell Biology International

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The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the gastrulation stages of chicken embryos on the ventral side of the epiblast at the cranial and lateral borders of the area pellucida. The basal lamina of the area pellucida situated inside the fibrillar band enables the spreading and probably the locomotion of dissociated cells, which appeared as polarized cells. Numerous cells were also found on the fibrillar band, and these cells lacked distinct lamellae and a polarized shape. Extensions of the cells contacted the extracellular fibrils and, at these sites of contact, the pattern of the fibrils was frequently deformed. From these observations and from previous results emerged the concept that spreading and locomotion of dissociated hypoblast cells, as well as single mesoblast cells and healing hypoblast epithelium, are inhibited by the band of extracellular fibrils, which acts as a physical barrier. The cell biological basis of the mechanism by which extracellular fibrils associated with the basal lamina arrest the migration of hypoblast and mesoblast cells, but guide the migration of primordial germ cells, is discussed.

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Isolation of the primordial germ cells from the giant freshwater prawn,Macrobrachium rosenbergii

Damrongphol

Ykarnjanasin

Poolsanguan

2000

Invertebrate Reproduction & Development

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Isolation of chick primordial germ cells from stages 4–8 embryos

Cited by 4 publications

References 18 publications

Human cumulus cell complexes studied in vitro by light microscopy and scanning electron microscopy

Human cumulus cell complexes studied in vitro by light microscopy and scanning electron microscopy

Behaviour of dissociated hypoblast cells on the basal lamina and on extracellular fibrils in the gastrulating chicken embryo.

Isolation of the primordial germ cells from the giant freshwater prawn,Macrobrachium rosenbergii

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