Isolation of coagulase negative Enterococcous sp. strains from non-broiler chicken producing bacteriocin active against Staphylococcus aureus

Abstract

Lactic acid bacteria (LAB) were isolated from the bile, gizzard, intestine and caecum of the Malaysian domestic non-broiler chicken. Of the 56 isolates grown on MRS agar plates, 7 - namely, B3L3, B4L4, G5L5, B5L6, B10L7, I1L8, and C4L10 - showed inhibition against the methicillin resistant Staphylococcus aureus (MRSA). These isolates were all Gram positive cocci, non-spore forming, and catalase negative. The purified fraction from the three phase partitioning (TPP) method, employing t-butanol and ammonium sulfate, produced inhibitory zones of 8 to 18 mm in diameter on the indicator organism, Staphylococcus aureus (MRSA). The TPP purified fraction contained heat stable proteins of about 10 kDa in size and was inactivated by trypsin. Based on the partial rDNA sequences (>89% similarity), isolates B4L4 and G5L5 belonged to Enterococcus hirae; isolate B3L3 belonged to Enterococcus faecium; isolates B5L6, B10L7, I1L8 to Enterococcus faecalis; and isolate C4L10 to Enterococcus mundtii. These probiotic strains, exclusively isolated from the Malaysian non-broiler chicken, produced an antimicrobial protein or bacteriocin which was active against a pathogenic strain of methicillin resistant Staphylococcus aureus (MRSA).

Lactococcus Lactis Strains From Intestinal Organ of Black Tips Shark Carcharhinus Limbatus Producing Nisin-Like Bacteriocin Active Against Shrimp and Fish Pathogens (Vibrio Parahaemolyticus and Vibrio Alginolyticus)

“…The 10x PCR master mix at pH 8.3 contained 50 mM dNTP each (dATP, dCTP, dGTP and dTTP), 3 mM MgCl2, 100 mM KCl, 20 mM Tris-HCl, and 0.1 Uμl -1 Taq polymerase. The other parameters used in amplification cycle were similar as in our previous work (Yusuf & Hamid., 2013). After amplification, the fragments were purified and further sequenced (First BASE Laboratories Sdn.…”

Lactic acid bacteria with antimicrobial properties isolated from the instestines of japanese quail (Coturnix Coturnix Japonica)

“…For instance, the use of probiotic from strain Lactobacilus fermentum AD1 resulted in 14% weight gain in Japanese quail [7]. In addition to our previous works on LAB screening from various local sources such as fermented food [8], broiler chicken [9], rabbit [10] and catfishes [11]; this work reports on several LAB strains isolated from quail's intestine with the objective to identify the diversity of LABs in quail. Future exploration of this LABs as well as the antimicrobials they produce would enable their uses as probiotic sources for quails or poultry feeding.…”