Isolation of cyanide‐degrading bacteria and molecular characterization of its cyanide‐degrading nitrilase

Terada, Ayane; Komatsu, Daisuke; Ogawa, Takahiro; Flamandita, Darin; Sahlan, Muhamad; Nishimura, Masaharu; Yohda, Masafumi

doi:10.1002/bab.2095

Cited by 9 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike other bacteria, that require additional nutrients to be more efficient in the biodegradation process [87,88], this bacterium performed the biodegradation process at a pH adjusted to 10.5 so that the CN remained dissolved in the medium rather than transforming into HCN [89]. This parameter provides an advantage and is one of the most important factors during biodegradation treatment [30,90].…”

Section: Discussionmentioning

confidence: 99%

Cyanide Bioremediation by Bacillus subtilis under Alkaline Conditions

Cáceda Quiroz,

Fora Quispe,

Carpio Mamani

et al. 2023

Water

View full text Add to dashboard Cite

Cyanide (CN) is a toxic environmental pollutant generated by various industrial activities, necessitating the application of bioremediation techniques for its degradation. Biodegradation is a more cost-effective and environmentally friendly technique with high efficiency in CN removal. This study isolated cyanide-degrading bacteria from Tutupaca mining site soil from Tacna, Peru. Bacillus subtilis strain TT10s was selected for its exceptional capacity to rapidly and completely eliminate cyanide under alkaline conditions (pH 10.5), removing 1000 ppm cyanide within 48 h. A kinetic analysis revealed that the biodegradation follows second-order rate kinetics (k2 = 0.08649 mg/(mg·h), R2 = 0.96622), consistent with the literature attribution of the rate-limiting step to the inducible cyanide dihydratase enzyme, which converts cyanide into ammonia and formate via the Michaelis–Menten model. Fourier-transform infrared spectroscopy (FTIR) spectral analysis further corroborated this enzymatic mechanism, showing the disappearance of CN peaks coupled with the emergence of ammonia (NH) and formate (C=O) peaks. Quantitative kinetic modelling integrated with FTIR profiles and degradation curves implicates cyanide dihydratase as the key rate-controlling enzyme in alkaline cyanide biodegradation without the need for an extra carbon source, generating interest for future bioremediation applications in highly contaminated environments.

show abstract

Section: Discussionmentioning

confidence: 99%

Cyanide Bioremediation by Bacillus subtilis under Alkaline Conditions

Cáceda Quiroz,

Fora Quispe,

Carpio Mamani

et al. 2023

Water

View full text Add to dashboard Cite

show abstract

“…Additionally, pMAL-ureABC was expressed with 1 mM IPTG in LB medium added 10.0 mg/L urea for 72 h at 25 • C. After ultrasonic disruption, the supernatant was purified by Ni-chelating affinity chromatography through His-tag that existed in N-terminus of UreC. Also, pMAL-CNH was expressed with 1 mM IPTG in LB medium added 2.0 mg/L cyanide for 72 h at 20 • C [21]. After ultrasonic disruption, the supernatant was purified by Ni-chelating affinity chromatography through His-tag that existed in N-terminus of CNH.…”

Section: The Expression Of Ureabc and Cnhmentioning

confidence: 99%

“…Moreover, a S. cerevisiae MT-1 strain was isolated and applied to degrade 46.45% of cyanide during the simulative fermentation of Chinese liquor [ 17 ]. Thus, it is an ideal and efficient method for reducing EC through applying microorganisms with the degradative activity of EC precursors, though the current reported microorganisms that can degrade cyanide were mainly isolated from wastewater or soil [ 18 , 19 , 20 , 21 ]. There are few reports on the in situ isolation of strains from fermented grains in terms of the degradative activity of cyanide.…”

Section: Introductionmentioning

confidence: 99%

The High-Throughput Screening of Microorganisms to Eliminate Ethyl Carbamate in Chinese Liquor

Yin,

Li,

Chen

et al. 2024

Foods

View full text Add to dashboard Cite

Ethyl carbamate (EC) is a 2A classified carcinogen in Chinese liquor that has raised many problems regarding food safety. Applying microorganisms to control the content of EC precursors in fermented grains has been proven as an effective method to reduce EC in alcoholic beverages. However, the utilization of microorganisms to decrease the precursors of EC (urea and cyanide) is still incomplete in regard to Chinese liquor. Thus, it is necessary to isolate strains with the degradative activities of urea and cyanide. Herein, Bacillus sonorensis F3 and Bacillus licheniformis YA2 strains were isolated from the fermented grains through multiple rounds of high-throughput screening, and the degradative abilities in urea and cyanide reached 95.72% and 75.48%, respectively. In addition, the urease from the B. sonorensis F3 strain and the carbon nitrogen hydrolase from the B. licheniformis YA2 strain were identified by the heterogeneous expression in Escherichia coli. Then, both F3 and YA2 strains were combined at a ratio of 5:1 and applied to eliminate the EC in the simulated fermentation of Chinese liquor; as a result, 51.10% of EC was reduced without affecting the main composition of flavor substances. The obtained strains have great potential in terms of the improvement of quality and safety of Chinese liquor.

show abstract

“…Consequently, aerobic oxidative metabolism and phosphorylation are compromised, resulting in hypoxia. Thus, cells can no longer produce ATP aerobically to meet their energy requirements or perform their cellular functions [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Free Cyanide by a New Isolated Alkaliphilic Bacillus licheniformis Strain

Uribe-Ramírez,

Cristiani-Urbina,

Morales-Barrera

2023

Microbiology Research

View full text Add to dashboard Cite

Microbial treatment of free-cyanide-polluted wastewater is a cost-effective, efficient, and eco-friendly method. Free-cyanide-degrading microbial cultures were isolated from different sources using batch-enrichment culture techniques, with acetate as the carbon source. Five microbial cultures were able to tolerate and grow at 1500 mg/L free cyanide, which was used as the only nitrogen source under strongly alkaline conditions (pH = 11). Among them, one bacterial strain (B11) was selected for further study because of its high free-cyanide-biodegradation efficiency. Bacterial strain B11 was molecularly identified as Bacillus licheniformis CDBB B11. Free cyanide inhibited the growth rate of B. licheniformis CDBB B11 at initial cyanide concentrations >75 mg/L. Despite this, the bacterial strain demonstrated 100% cyanide-biodegradation efficiency at initial cyanide concentrations ranging from 25 to 75 mg/L, which decreased to 32% as the initial cyanide concentration increased from 75 to 1500 mg/L. Free-cyanide biodegradation corresponds to bacterial growth and ammonia accumulation in the culture medium. The alkaliphilic B. licheniformis CDBB B11 strain is a robust candidate for the detoxification of free-cyanide-laden wastewater because it tolerates and efficiently degrades free cyanide at concentrations of up to 1500 mg/L.

show abstract

Isolation of cyanide‐degrading bacteria and molecular characterization of its cyanide‐degrading nitrilase

Cited by 9 publications

References 31 publications

Cyanide Bioremediation by Bacillus subtilis under Alkaline Conditions

Cyanide Bioremediation by Bacillus subtilis under Alkaline Conditions

The High-Throughput Screening of Microorganisms to Eliminate Ethyl Carbamate in Chinese Liquor

Biodegradation of Free Cyanide by a New Isolated Alkaliphilic Bacillus licheniformis Strain

Contact Info

Product

Resources

About