Isolation of Dendritic Cells in the Rat Liver Lymph

Matsuno, Kenjiro; Kudo, Shunsuke; Ezaki, Taichi; Miyakawa, Kazuhisa

doi:10.1097/00007890-199510150-00027

Cited by 42 publications

(42 citation statements)

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“…To determine whether pDCs behave similarly, we removed MLNs or the liver-draining celiac LNs from young rats, creating MLNX and CoeLNX rats, respectively. By 6 wk, the afferent and efferent lymph vessels have anastamosed, which permits collection of pseudoafferent intestinal or liver lymph cells from the thoracic duct (41)(42)(43). As found in nonlympadenectomized rats, we could identify a population of MHC-II ϩ CD45RA Ϫ TCR␣␤ Ϫ Ig Ϫ TDLs, in both MLNX and CoeLNX lymph, that was CD45R…”

Section: Efferent Lymph As Well As Pseudoafferent Intestinal and Hepamentioning

confidence: 60%

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Plasmacytoid Dendritic Cells Do Not Migrate in Intestinal or Hepatic Lymph

Yrlid

Cerovic

Milling

et al. 2006

The Journal of Immunology

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Plasmacytoid dendritic cells (pDCs) recognize pathogen-associated molecules, particularly viral, and represent an important mechanism in innate defense. They may however, also have roles in steady-state tolerogenic responses at mucosal sites. pDCs can be isolated from blood, mucosa, and lymph nodes (LNs). Although pDCs can express peripherally derived Ags in LNs and at mucosal sites, it is not clear whether pDCs actually migrate from the periphery in lymph or whether LN pDCs acquire Ags by other mechanisms. To determine whether pDCs migrate in lymph, intestine or liver-draining LNs were removed and thoracic duct leukocytes (TDLs) were collected. TDLs expressing MHC-II and CD45R, but not TCRαβ or CD45RA, were then analyzed. These enriched TDLs neither transcribe type I IFNs nor secrete inflammatory cytokines in response to viral stimuli in vitro or after a TLR7/8 stimulus in vivo. In addition, these TDLs do not express CD5, CD90, CD200, or Siglec-H, but do express Ig, and therefore represent B cells, despite their lack of CD45RA expression. Intestinal and hepatic lymph are hence devoid of bona fide pDCs under both steady-state conditions and after TLR7/8 stimulation. This shows that any role for pDCs in Ag-specific T cell activation or tolerance must differ from the roles of classical dendritic cells, because it cannot result from peripheral Ag capture, followed by migration of pDCs via lymph to the LN.

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Section: Efferent Lymph As Well As Pseudoafferent Intestinal and Hepamentioning

confidence: 60%

“…Rats used were males of 12-24 wk of age. MLNX, CoeLNX, and thoracic duct cannulation were performed, as described previously (41)(42)(43). All procedures were conducted in accordance with Home Office guidelines.…”

Section: Animals and Surgical Proceduresmentioning

confidence: 99%

Plasmacytoid Dendritic Cells Do Not Migrate in Intestinal or Hepatic Lymph

Yrlid

Cerovic

Milling

et al. 2006

The Journal of Immunology

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“…Rats used were males 12-24 wks of age. Mesenteric and coeliac lymphadenectomy (MLNX and CoeLNX, respectively) and thoracic duct cannulation were performed as described previously [23,31]. All procedures were carried out in accordance with Home Office guidelines.…”

Section: Methodsmentioning

confidence: 99%

A distinct subset of intestinal dendritic cells responds selectively to oral TLR7/8 stimulation

Yrlid¹,

Cerovic

Milling³

et al. 2006

Eur J Immunol

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The intestinal innate immune system continually interacts with commensal bacteria, thus oral vaccines should induce extra/alternative activation of DC, potentially through TLR. To examine this we collected intestinal lymph DC (iL-DC) under steady-state conditions and after feeding resiquimod (R-848), a synthetic TLR7/8 ligand, which we showed induces complete emptying of gut DC into lymph. iL-DC are heterogeneous with subset-specific functions. In this study we determined the kinetics of iL-DC subset release, activation and cytokine secretion induced by R-848. We show that L-DC comprise three distinct subsets (CD172a high , CD172a int and CD172a low ) present with similar frequencies in intestinal but not hepatic lymph. No iL-DC express TLR7 mRNA, and only CD172a + iL-DC express TLR8. However, after oral R-848 administration, output of all three subsets increases dramatically. CD172a high DC release precedes that of CD172a low DC, and the increased frequency of CD25 high iL-DC is restricted to the two CD172a + subsets. After feeding R-848 only CD172a high iL-DC secrete IL-6 and IL-12p40. However, CD172a int and CD172a high DC secrete similar but markedly lower amounts when stimulated in vitro. These results highlight the importance of in vivo approaches to assess adjuvant effects on DC and give novel insights into the subset-specific effects of an oral TLR ligand on intestinal DC.

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“…12 The liver-draining LNx included the portal and celiac LNs. Splenectomies were performed according to the same protocol for the lymphadenectomies.…”

Section: Lymphadenectomy Of Liver-draining Lns and Splenectomymentioning

confidence: 99%

Characterization of the liver-draining lymph nodes in mice and their role in mounting regional immunity to HBV

Zheng

Tian

2013

Cell Mol Immunol

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The lymphatic system is important in mounting an immune response to foreign antigens and tumors in humans and animal models. The liver produces a large amount of lymph, and its lymphatic system is divided into three major components: the portal, sublobular and superficial lymphatic vessels. Despite the fact that mice are the most commonly used laboratory animals, detailed descriptions of the anatomical location and function of the lymph nodes (LNs) that drain the liver are surprisingly absent. In this study, we found that the portal and celiac LNs adjacent to mouse liver were stained with Evans blue within 5-8 min. Enhanced green fluorescence protein (EGFP)-positive cells from the liver also drained into the two aforementioned LNs. These data indicate that the portal and celiac LNs drain the mouse liver. Lymphadenectomy of the identified liver-draining LNs resulted in hepatitis B virus (HBV) persistence in immunocompetent mice compared with the sham group. In addition, the frequencies of CD81 T cells and dendritic cells (DCs) increased significantly in the liver-draining LNs after hydrodynamic injection of HBV plasmid. Liver-draining LN cells in HBV plasmid-injected mice also showed significant antigen-specific proliferation in response to stimulation with recombinant hepatitis B core antigen in vitro. Adoptive transfer of these cells into Rag1 2/2 mice induced a reduction in the serum concentration of hepatitis B surface antigen (HBsAg) compared to liver-draining LN cells in uninjected mice. Altogether our data characterize the liver-draining LNs and provide evidence that the liver-draining LNs induce an anti-HBV-specific immune response responsible for HBV clearance.

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Isolation of Dendritic Cells in the Rat Liver Lymph

Cited by 42 publications

References 0 publications

Plasmacytoid Dendritic Cells Do Not Migrate in Intestinal or Hepatic Lymph

Plasmacytoid Dendritic Cells Do Not Migrate in Intestinal or Hepatic Lymph

A distinct subset of intestinal dendritic cells responds selectively to oral TLR7/8 stimulation

Characterization of the liver-draining lymph nodes in mice and their role in mounting regional immunity to HBV

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