Isolation of detergent-resistant membranes from plant photosynthetic and non-photosynthetic tissues

Carmona-Salazar, Laura; Hafidi, Mohammed El; Enríquez-Arredondo, Consuelo; Vázquez-Vázquez, Christian; Vara, Luis E. González de la; Gavilanes‐Ruíz, Marina

doi:10.1016/j.ab.2011.05.044

Cited by 27 publications

(19 citation statements)

References 58 publications

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“…Borner et al [4] and Mongrand et al [31] first quantified lipid class and fatty acid composition using DRM as microdomain-enriched fractions. In the same way, the DRM lipid compositions of maize embryos and bean leaves have been characterized [6,7]. Furt et al [12] reported that phosphatidylinositol-4,5-bisphosphate forms a cluster-like structure that is not influenced by sterol depletion, and that phosphoinositide metabolism-related enzyme activities in DRMs are higher than in the PM.…”

Section: Introductionmentioning

confidence: 99%

Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

et al. 2016

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Cold acclimation (CA) results in alteration of the plasma membrane (PM) lipid composition in plants, which plays a crucial role in the acquisition of freezing tolerance via membrane stabilization. Recent studies have indicated that PM structure is consistent with the fluid mosaic model but is laterally non-homogenous and contains microdomains enriched in sterols, sphingolipids and specific proteins. In plant cells, the function of these microdomains in relation to CA and freezing tolerance is not yet fully understood. The present study aimed to investigate the lipid compositions of detergent resistant fractions of the PM (DRM) which are considered to represent microdomains. They were prepared from leaves of low-freezing tolerant oat and high-freezing tolerant rye. The DRMs contained higher proportions of sterols, sphingolipids and saturated phospholipids than the PM. In particular, one of the sterol lipid classes, acylated sterylglycoside, was the predominant sterol in oat DRM while rye DRM contained free sterol as the major sterol. Oat and rye showed different patterns (or changes) of sterols and 2-hydroxy fatty acids of sphingolipids of DRM lipids during CA. Taken together, these results suggest that CA-induced changes of lipid classes and molecular species in DRMs are associated with changes in the thermodynamic properties and physiological functions of microdomains during CA and hence, influence plant freezing tolerance.

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Section: Introductionmentioning

confidence: 99%

Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

et al. 2016

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“…With a reported size of microdomains ranging from nanoscale up to microscale (Edidin, 2001; Cacas et al, 2012), direct visualization of such domains in living cells remains challenging. Sterol-dependent protein localization was reported in various plant systems (Borner et al, 2005; Roche et al, 2008; Kierszniowska et al, 2009; Minami et al, 2009; Carmona-Salazar et al, 2011; Navarro-Lérida et al, 2012) and selected proteins with sterol-dependent membrane location were shown to exhibit a patchy organization within the plasma membrane (Bariola et al, 2004; Parton and Hancock, 2004). The biological function of these microdomains was especially linked to signaling and transport processes in several independent studies (Simons and Toomre, 2000; Borner et al, 2005; Langhorst et al, 2005; Kierszniowska et al, 2009; Staubach and Hanisch, 2011; Stuermer, 2011).…”

Section: Introductionmentioning

confidence: 99%

Plasma membrane lipidâ€“protein interactions affect signaling processes in sterol-biosynthesis mutants in Arabidopsis thaliana

et al. 2014

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The plasma membrane is an important organelle providing structure, signaling and transport as major biological functions. Being composed of lipids and proteins with different physicochemical properties, the biological functions of membranes depend on specific protein–protein and protein–lipid interactions. Interactions of proteins with their specific sterol and lipid environment were shown to be important factors for protein recruitment into sub-compartmental structures of the plasma membrane. System-wide implications of altered endogenous sterol levels for membrane functions in living cells were not studied in higher plant cells. In particular, little is known how alterations in membrane sterol composition affect protein and lipid organization and interaction within membranes. Here, we conducted a comparative analysis of the plasma membrane protein and lipid composition in Arabidopsis sterol-biosynthesis mutants smt1 and ugt80A2;B1. smt1 shows general alterations in sterol composition while ugt80A2;B1 is significantly impaired in sterol glycosylation. By systematically analyzing different cellular fractions and combining proteomic with lipidomic data we were able to reveal contrasting alterations in lipid–protein interactions in both mutants, with resulting differential changes in plasma membrane signaling status.

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“…The plant plasma membrane contains at least four major sterols: stigmasterol, sitosterol, campesterol, and cholesterol. For example, sitosterol and stigmasterol represent the major sterols in Arabidopsis and rice (Cooke et al 1991(Cooke et al , 1993Arnqvist et al 2008), whereas sitosterol and campesterol are two of the major sterols present in rye and maize (Cooke et al 1991(Cooke et al , 1993Bohn et al 2001;Carmona-Salazar et al 2011) and stigmasterol and cholesterol are found at the highest levels in oat (Cooke et al 1991(Cooke et al , 1993(Cooke et al , 1994. Sterols have a stabilizing effect on the membrane structure since their small polar head (the OH in the position 3 0 ) and the planar stereochemistry of their rings fulfill the voids between the other complex lipids due to bulky polar heads and unsaturations that produce deformations of acyl chains.…”

Section: Interaction With Sterols: the Planar Solutionmentioning

confidence: 99%

“…Attached to the sphingosine there is a neutral head composed of carbohydrates or an acidic head with phosphate and carbohydrates. The diversity of these two large classes of membrane complex lipids lies in the many forms of FA, LCB, and polar heads that may assemble in multiple combinations, rendering a large amount of different molecular species (Bohn et al 2001;Chen et al 2009;Carmona-Salazar et al 2011, 2015. Sterols are also diversified, as they are mainly present as sitosterol, stigmasterol, campesterol, and cholesterol (Hartmann 2004).…”

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confidence: 99%

Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase

et al. 2015

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Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The plasma membrane H(+)-ATPase, an enzyme from plant cells, is an excellent example of a transmembrane protein whose activity is influenced by the hydrophilic compartments at both sides of the membrane and by the hydrophobic domains of the lipid bilayer. As a result, an extensive documentation of the effect of numerous amphiphiles in the enzyme activity can be found. Detergents, membrane glycerolipids, and sterols can produce activation or inhibition of the enzyme activity. In some cases, these effects are associated with the lipids of the membrane bulk, but in others, a direct interaction of the lipid with the protein is involved. This review gives an account of reports related to the action of the membrane lipids on the H(+)-ATPase activity.

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Isolation of detergent-resistant membranes from plant photosynthetic and non-photosynthetic tissues

Cited by 27 publications

References 58 publications

Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance

Plasma membrane lipidâ€“protein interactions affect signaling processes in sterol-biosynthesis mutants in Arabidopsis thaliana

Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase

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