Isolation of embryonic cell‐lines from porcine blastocysts

Gerfen, R. W.; Wheeler, M. B.

doi:10.1080/10495399509525828

Cited by 56 publications

(43 citation statements)

References 19 publications

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“…In a present study, the morphology of BuES-like cells on BEF was observed to be similar to our previous study [16], and closely resembled porcine [39] and cat [30] ES cells. However, the colony morphology was found to vary on heterologous feeder layers where they appeared compact, thinner and flatter but with clear margins on GEF feeder layers and appeared to carry loose cells with increased intercellular spacing and showed lack of clear margins on SEF feeder layers.…”

Section: Discussionsupporting

confidence: 89%

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

Kumar

Anand

Singh

et al. 2011

J Assist Reprod Genet

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Purpose The aim of the present study is to compare the ability of homologous and heterologous embryonic fibroblast feeder layers to support isolation and proliferation of buffalo ES-like cells generated from hatched and expanded blastocysts produced by in vitro fertilization and characterization of derived cells through expression of pluripotent markers. Methods Embryonic stem cells were derived from hatched and expanded blastocysts through intact blastocyst culture and enzymatic method respectively and compared for proliferation rate on homologous (buffalo) and heterologous feeder layers (goat and sheep). Results A total of 69 hatched and 83 expanded blastocysts were used for isolation of inner cell masses which were seeded on buffalo, goat and sheep embryonic feeder layers. Following seeding, attachment rate, primary colony formation rate and survival to maximum number of passages were observed to be higher on homologous feeder layers.Conclusions Upon comparison of different feeder layer cells for derivation and maintenance of buffalo ES-like cells from hatched and expanded blastocysts, buffalo embryonic fibroblast cells were able to provide a better environment for maintaining pluripotency in culture conditions.

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Section: Discussionsupporting

confidence: 89%

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

Kumar

Anand

Singh

et al. 2011

J Assist Reprod Genet

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“…Establishment of a similar system in the pig would be very useful for the production of transgenic animals devoid of undesirable antigens for xenotransplantaion. However, putative ES cells in swine have been obtained by many authors to possess some typical characteristics of pluripotent cells in short term cultures without establishing any stable cell lines [6]. So far, the only successful establishment of ES cell lines from porcine prcimplantation embryos and the demonstration of their in vivo pluripotency was the work of Chen et al [7].…”

Section: Introductionmentioning

confidence: 99%

The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine

Tsung

Ren

et al. 2003

Cell Res

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As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation of embryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishment of two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 and a 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, marker characterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentially useful for further basic studies.

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“…There have been many attempts to gain ES cell lines in such mammalian species as hamster [10], pig [11][12][13], cow [11,14], mink [15], sheep [12], rabbit [16], rat [17], monkey [18] and human [19]. However, no ES-like cells mentioned distinctly contributed chimeras.…”

mentioning

confidence: 99%

Effects of Feeder Cells on the Establishment of ES Cells from Mice and Rats. The Difference between Allogenetic and Xenogenetic Feeders.

Kawase

Yoshimizu

Hashimoto

2000

Journal of Reproduction and Development

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Abstract. The technology of mouse embryonic stem (ES) cells is clearly proving to be a very successful method for producing transgenic mice. Although there has been considerable efforts to establish ES cell lines from other animals, most of all lines obtained, so far, have been derived from mice. Here, we examined effects of allogenetic and xenogenetic feeders on the establishment of ES cells from mice and rats. We first used three types of feeder cells on the establishment of rat ES cells; mouse primary embryonic fibroblsts (M-PEF), rat primary embryonic fibroblasts (R-PEF) and a mouse embryonic fibroblast cell line, SL10. We could gain some rat ES cell lines only on R-PEF. RES-DA1 cell line from DA rat strain, has the same morphology as mouse ES cells, alkaline phosphatase activity and the 4C9 antigen. We, moreover, examined effects of M-PEF and R-PEF feeders on the establishment of mouse ES cells in C57BL/6 mice. The mouse ES cell line could be established only on M-PEF feeders. Both rat and mouse ES cell lines had pluripotenciality. These results suggested that the establishment of ES cell lines is more effective on allogenetic feeder cells than on xenogenetic cells.

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Isolation of embryonic cell‐lines from porcine blastocysts

Cited by 56 publications

References 19 publications

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells

The culture and establishment of embryonic germ (EG) cell lines from Chinese mini swine

Effects of Feeder Cells on the Establishment of ES Cells from Mice and Rats. The Difference between Allogenetic and Xenogenetic Feeders.

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