Isolation of Endophytic
            <i>Streptomyces</i>
            Strains from Surface-Sterilized Roots

Sardi, P.; Saracchi, M.; Quaroni, S.; Petrolini, B.; Borgonovi, G. E.; Merli, Stefania

doi:10.1128/aem.58.8.2691-2693.1992

Cited by 167 publications

(71 citation statements)

References 14 publications

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“…identified by morphological characteristics were the most frequent isolates among the actinobacterial endoflora of banana roots in the present study. Similar results were found in field crops and Italian native plants [5], wheat roots from a range of sites across South Australia [15] and tomato roots in South China [13]. The majority of antagonistic streptomycetes found by the antibiosis assay have similar morphological characteristics (similar to S. griseorubiginosus), results consistent with our previous findings from the other banana plantation [11].…”

Section: Discussionsupporting

confidence: 88%

“…The first endophytic actinomycetes studied were Frankia strains isolated from non-leguminous plants [4]. Other endophytic actinomycetes such as Streptomyces, Streptoverticillium, Nocardia, Micromonospora, Microbispora, and Streptosporangium strains were isolated from surface-sterilized roots of different plant species in Italy [5] and of maize in Brazil [6]. The use of endophytic actinomycetes as biological control agents of soil-borne root disease is of interest through their ability to colonize healthy plant tissue and produce antibiotics in situ [7].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots

Cao¹,

Qiu²,

You³

et al. 2005

FEMS Microbiology Letters

238

121

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A total of 131 endophytic actinomycete strains were successfully isolated from surface-sterilized banana roots. These isolates belonged to Streptomyces (n=99), Streptoverticillium (n=28), and Streptosporangium (n=2) spp. The remaining 2 isolates were not identified. About 18.3% of the isolates inhibited the growth of pathogenic Fusarium oxysporum f. sp. cubense on banana tissue extract medium. The most frequently isolated Streptomyces sp. strain S96 was similar to Streptomyces griseorubiginosus. About 37.5% of the S. griseorubiginosus strains were antagonistic to F. oxysporum f. sp. cubense. The antagonism of strain S96 was lost when FeCl(3) was introduced into the inhibition zone. In vivo biocontrol assays showed that the disease severity index (DSI) was significantly (P=0.05) reduced and mean fresh weight increased (P=0.001) in plantlets treated with strain S96 compared to those grown in the absence of the biocontrol strain. These findings indicate the potential of developing siderophore-producing Streptomyces endophytes for the biological control of fusarium wilt disease of banana.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots

Cao¹,

Qiu²,

You³

et al. 2005

FEMS Microbiology Letters

238

121

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“…Thirty-five healthy cucumber seedlings (3-week old) collected from the farm described above were uprooted and transferred to the laboratory in cooled containers for immediate processing. Roots were surface-disinfested as described by Sardi et al (1992) and the roots were macerated as described by Hallmann et al (1997b). After filtering the slurry through sterile cotton cloth, the filtrate was serially diluted (10 )2 , 10 )3 and 10 )4 ) and aliquots (0AE2 ml) were spread with a sterile glass rod over the surface of humic acid-vitamin agar (HVA) medium (Hayakawa and Nonomura 1987) amended with cycloheximide (50 lg ml )1 ; Sigma Chemical Co., St Louis, MO, USA) and nystatin (50 lg ml )1 ; Sigma).…”

Section: Isolation Of Endophytic Actinomycetesmentioning

confidence: 99%

“…Endophytic streptomycete actinomycetes (SA) and nonstreptomycete actinomycetes (NSA; Sardi et al 1992;Coombs and Franco 2003;El-Tarabily 2003;Rosenblueth and Martinez-Romero 2006) have been isolated from within live tissues of various plant species. Endophytic actinomycetes have been shown to protect plants against different soil-borne plant pathogens including Rhizoctonia solani and Verticillium dahliae (Krechel et al 2002), Plectosporium tabacinum (El-Tarabily 2003), Gaeumannomyces graminis var.…”

Section: Introductionmentioning

confidence: 99%

Plant growth promotion and biological control ofPythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes

El‐Tarabily

Nassar

Hardy

et al. 2009

Journal of Applied Microbiology

235

108

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Aims: To evaluate the potential of Actinoplanes campanulatus, Micromonospora chalcea and Streptomyces spiralis endophytic in cucumber roots, to promote plant growth and to protect seedlings and mature plants of cucumber from diseases caused by Pythium aphanidermatum, under greenhouse conditions. Methods and Results: Three endophytic isolates, out of 29, were selected through tests aimed at understanding their mechanisms of action as biocontrol agents and plant growth promoters. When applied individually or in combination, they significantly promoted plant growth and reduced damping‐off and crown and root rot of cucumber. The combination of the three isolates resulted in significantly better suppression of diseases and plant growth promotion, than where the plants were exposed to individual strains. Conclusions: The three selected actinomycete isolates colonized cucumber roots endophytically for 8 weeks, promoted plant growth and suppressed pathogenic activities of P. aphanidermatum on seedling and mature cucumber plants. Significance and Impact of the Study: The results clearly show that the endophytic, glucanase‐producing actinomycetes used, especially as a combined treatment, could replace metalaxyl, which is the currently recommended fungicide for Pythium diseases in the United Arab Emirates. These endophytic isolates also have the potential to perform as plant growth promoters, which is a useful attribute for crop production in nutrient impoverished soils.

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“…and Streptosporangium spp. were isolated from roots of different plant species in Italy and from maize in Brazil that showed antagonistic activities against Grampositive bacteria and fungi (Sardi et al 1992;Araujo et al 2000). A new naphthoquinone antibiotic has been identified from an endophytic Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of endophytic Streptomyces strains from surface-sterilized tomato (Lycopersicon esculentum) roots

Cao

Qiu

You

et al. 2004

Lett Appl Microbiol

154

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Aims: To isolate endophytic Streptomyces strains from tomato and examine their antimicrobial activity. Methods: Endophytic Streptomyces strains were isolated using surface-sterilization methods and identified by morphological characteristics. Antimicrobial activities were measured by the agar plate sensitivity method. Antifungal activity in vivo was measured by seedling mortality in infested soils. Results: Twenty-one per cent of endophytic streptomycete isolates produced antibacterial metabolites and 41% produced antifungal metabolites in S medium. Sixty-five per cent of the most frequently isolated strains inhibited the growth of Rhizoctonia solani by the antibiosis assay but only 32% produced metabolites active against R. solani in S medium. Growth promotion and enhanced disease resistance of seedlings inoculated with Streptomyces sp. strain S30 were observed in tomato but not in cucumber seedlings. Conclusions: Endophytic Streptomyces spp. strains were successfully isolated using stringent methods and strain S30 promoted growth and enhanced resistance to R. solani in tomato seedlings. Significance and Impact of the Study: Endophytic streptomycetes showing antifungal activity in vitro and in vivo may indicate the potential for their use as biocontrol agents particularly of R. solani disease of tomato seedlings.

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Isolation of Endophytic Streptomyces Strains from Surface-Sterilized Roots

Abstract: When the roots of 28 plant species were surface sterilized and incubated on agar medium, endophytic actinomycetes in the root cortex were observed by direct microscopic observation and pure culture techniques.

Cited by 167 publications

References 14 publications

Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots

Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots

Plant growth promotion and biological control ofPythium aphanidermatum, a pathogen of cucumber, by endophytic actinomycetes

Isolation and characterization of endophytic Streptomyces strains from surface-sterilized tomato (Lycopersicon esculentum) roots

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