Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens

Abstract

Among different pathogens, enterotoxic E. coli (ETEC) has been for many years an important etiological agent in the occurrence of digestive system disease of newborn animals. In counties with developed pig breeding (farming), including our country, diarrhea in the neonatal period, caused by ETEC strains is one of the most present and economically most significant diseases. The aim of this investigation wais to determine the prevalence of ETEC strains in piglets (weaning pigs), originated from 5 (five) pig farms in the Republic of Serbia, as well as their serological typization based on characteristics of somatic O antigens, presence of fimbrial antigensadhesins and hemolytic activity. The material for this investigation was targeted and sampled from piglets that have shown clinical signs of neonatal diarrhea or pathoanatomical changes characteristic for enteritis caused by ETEC strains. The total number of isolated ETEC strains were 148, of which 91 (61.48 %) were determined on the basis of somatic O antigen characteristics. The largest number of strains, 42 (46.15 %) belonged to serotype O149. Serological types O8 and O147 were represented, each with 15 strains (16.48 %). In 13 (14.28 %) strains the somatic antigen which belonged to serotype O138 was determined and in 6 (6.59 %) strains the antigen belonged to serotype O157. No strain agglutinated with hyperimmune O139 serotype serum. The presence of fimbrial adhesins was determined in 47 (51.64%) strains and of that number the F4 type of fimbrial adhesins was detected in 38 (80.85 %) strains. The presence of F5 adhesins was determined in 4, and F6 in 3. In 2 strains, the paralell presence of two adhesin types, F4 and F6 was detected. The greatest number of strains 30 (71.42 %) with adhesin F4 belonged to O149 serotype, a considerably smaller number, 4 (26.66%) to serotype O8, 2 strains to serotype O157 and to each serotype O147 and O138 1 strain. The fimbrial adhesin of F5 type was detected in 3 strains which belonged to serotype O8 and in 1 strain of serotype O149. All 3 strains with F6 adhesin, belonged to serotype O8. From 2 strains which had, at the same time, adhesins F4 and F6 one belonged to serotype O8 and the other to serotype O138. Hemolytic activity was present in 42 (46.15 %) strains, of which 34 strains belonged to O149 serotype, 6 strains to O157 serotype and 2 strains to O147 serotype

The role of Escherichia coli in the etiology of piglet diarrhea in selected pig producing districts of central Uganda

“…The finding of 18.4% of the E. coli with virulence factors (fimbriae and/or enterotoxin genes) is significant since it confirms the possible involvement of these bacteria in the etiology of diarrhea and piglet mortalities in Uganda. In the present study, the only adhesin detected in E. coli was F4, which is in agreement with the previous study conducted in Northern and Eastern Uganda by Ikwap et al, (5) but differ from developed countries (14) where F4 was most predominant. In our study, F18 adhesin was not detected, however, F18 adhesin was recently reported in diarrheic weaners from large commercial farms in Central Uganda (15).…”

Electrochemical magneto immunosensor based on endogenous β-galactosidase enzyme to determine enterotoxicogenic Escherichia coli F4 (K88) in swine feces using square wave voltammetry

“…ETEC F4 must be isolated by selective culture medium and characterized by biochemical tests. For this purpose, conventional immunological techniques such as agglutination, and ELISA [8][9][10] or molecular techniques such as PCR [11][12][13][14] are used to identify virulence factors. PCR has remarkable advantages over culture techniques and other standard techniques such as specificity and sensitivity.…”