Isolation of exosomes from whole blood by integrating acoustics and microfluidics

Wu, Mengxi; Ouyang, Yi‐Bing; Wang, Zeyu; Zhang, Rui; Huang, Po‐Hsun; Chen, Chuyi; Li, Hui; Li, Peng; Quinn, David; Dao, Ming; Suresh, S.; Sadovsky, Yoel; Huang, Tony Jun

doi:10.1073/pnas.1709210114

Cited by 751 publications

(620 citation statements)

References 46 publications

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“…Recently several groups have described using microfluidics combined with acoustic sorting . This approach uses microfluidic devices that are modified by using applied acoustic fields to exert forces on EVs that allow their separation from other biological components.…”

Section: Methods For Isolation/characterization Of Evs From the Gcfmentioning

confidence: 99%

Extracellular vesicle identification in tooth movement models

Holliday

Truzman

Zuo

et al. 2019

Orthod Craniofacial Res

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Structured Abstract Extracellular vesicles (EVs) are 30‐150 nm in diameter vesicles released by cells that serve important intercellular regulatory functions. EVs include exosomes and microvesicles. Exosomes form in multivesicular bodies and are released extracellularly as the multivesicular bodies fuse with the plasma membrane. Microvesicles bud directly from the plasma membrane. Here, we examine methods that are available or emerging to detect and study EVs during orthodontic tooth movement (OTM). EV's involvement in regulating bone remodelling associated with OTM may be demonstrated by adding isolated EVs to an animal model to change the rate of tooth movement. Exosomes in multivesicular bodies might be detected by immunogold labelling of markers in sections from the tooth and jaw and detection by electron microscopy. Gingival crevicular fluid (GCF) is enriched in EVs. Detection and characterization of EVs released by osteoclasts during resorption have been described, and this information could be used to analyse EVs in OTM models. Regulatory EVs may be enriched in the GCF from teeth that are being moved or are undergoing root resorption. Emerging approaches, including nanoparticle tracking, ExoView and micro‐ and nanofluidics, show promise for studying EVs in the GCF. Techniques that amplify signal, including polymerase chain reaction (PCR), provide the sensitivity necessary to utilize EVs from GCF as biomarkers. Studies of the role of EVs in OTM will provide fresh insight that may identify means for enhancing OTM procedures. EVs in GCF may include biomarkers for bone remodelling during OTM, orthodontic‐associated root resorption, and other dental pathologies.

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Section: Methods For Isolation/characterization Of Evs From the Gcfmentioning

confidence: 99%

Extracellular vesicle identification in tooth movement models

Holliday

Truzman

Zuo

et al. 2019

Orthod Craniofacial Res

View full text Add to dashboard Cite

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“…This method has been used to separate nanoscale vesicles (<200 nm) from cell culture . Recently, Wu et al proposed a microfluidics‐based two‐stage acoustic separation strategy for separation of exosomes from whole blood ( Figure ) . In the first separation stage, red blood cells (RBCs), WBCs, and platelets (PLTs) are removed.…”

Section: Separation Techniques For Exosomesmentioning

confidence: 99%

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Lin

Chen

et al. 2019

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Exosomes are secreted by most cell types and circulate in body fluids. Recent studies have revealed that exosomes play a significant role in intercellular communication and are closely associated with the pathogenesis of disease. Therefore, exosomes are considered promising biomarkers for disease diagnosis. However, exosomes are always mixed with other components of body fluids. Consequently, separation methods for exosomes that allow high‐purity and high‐throughput separation with a high recovery rate and detection techniques for exosomes that are rapid, highly sensitive, highly specific, and have a low detection limit are indispensable for diagnostic applications. For decades, many exosome separation and detection techniques have been developed to achieve the aforementioned goals. However, in most cases, these two techniques are performed separately, which increases operation complexity, time consumption, and cost. The emergence of microfluidics offers a promising way to integrate exosome separation and detection functions into a single chip. Herein, an overview of conventional and microfluidics‐based techniques for exosome separation and detection is presented. Moreover, the advantages and drawbacks of these techniques are compared.

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“…Wu et al employed an acoustofluidics technique that consisted of two modules: a cell-removal module and an EV-isolation module [101]. This two-step device greatly simplifies the pre-processing of complex samples consisting of cells such as blood [101]. However, further studies in this area are still needed.…”

Section: Microfluidics-based Ev Isolationmentioning

confidence: 99%

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

Guo

Tao

Dawn

2018

J of Extracellular Vesicle

150

111

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Extracellular vesicles (EVs), which can be found in almost all body fluids, consist of a lipid bilayer enclosing proteins and nucleic acids from their cells of origin. EVs can transport their cargo to target cells and have therefore emerged as key players in intercellular communication. Their potential as either diagnostic and prognostic biomarkers or therapeutic drug delivery systems (DDSs) has generated considerable interest in recent years. However, conventional methods used to study EVs still have significant limitations including the time-consuming and low throughput techniques required, while at the same time the demand for better research tools is getting stronger and stronger. In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advantages, including low cost, low sample volumes, high throughput and precision. This review summarizes recent advances in microfluidics-based technologies, compares conventional and microfluidics-based technologies, and includes a brief survey of recent progress towards integrated “on-a-chip” systems. In addition, this review also discusses the potential clinical applications of “on-a-chip” systems, including both “liquid biopsies” for personalized medicine and DDS devices for precision medicine, and then anticipates the possible future participation of cloud-based portable disease diagnosis and monitoring systems, possibly with the participation of artificial intelligence (AI).

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Isolation of exosomes from whole blood by integrating acoustics and microfluidics

Cited by 751 publications

References 46 publications

Extracellular vesicle identification in tooth movement models

Extracellular vesicle identification in tooth movement models

Progress in Microfluidics‐Based Exosome Separation and Detection Technologies for Diagnostic Applications

Microfluidics‐based on‐a‐chip systems for isolating and analysing extracellular vesicles

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