Isolation of Feline Parvovirus from Peripheral Blood Mononuclear Cells of Cats in Northern Vietnam

Abstract

Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin -2 -dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of VN antibodies in the cats.

Role of Multiple Hosts in the Cross-Species Transmission and Emergence of a Pandemic Parvovirus

“…1 and 3), and hence must have arisen more than once in the raccoon viruses derived from CPV. The importance of the 300Asp change for CPV-derived viruses in hosts other than dogs is confirmed by the finding of this mutation in some viruses from leopard cats in Southeast Asia and in a CPV-2 isolate that had been passaged in cultured feline cells (11,14,22). The variants at residues 297 and 305 also have complex evolutionary patterns.…”

Comparison of Prevalence of Feline Herpesvirus Type 1, Calicivirus and Parvovirus Infections in Domestic and Leopard Cats in Vietnam

“…Previously we conducted a serosurvey for the viruses in domestic and leopard cats from Hanoi area in northern Vietnam in 1997 [8,10]. In the present study, we expanded the study and collected samples of cats from Ho Chi Minh City (HCM) area in southern Vietnam and compared the results with our previous reports in northern Vietnam.…”

“…The results of this survey together with our previous reports [8,10] were summarized in Table 1. In Hanoi area, a positive rate for FHV-1 antibody was only 1.4%.…”

“…The antibodies against FHV-1, FCV and FPV were tested by the indirect immunofluorescence assay (IFA) as described previously [8]. Plasma samples were screened at a dilution of 1:50 in the IFA.The results of this survey together with our previous reports [8,10] were summarized in Table 1. In Hanoi area, a positive rate for FHV-1 antibody was only 1.4%.…”

Monoclonal Antibodies That Distinguish Antigenic Variants of Canine Parvovirus

“…CPV-2 Cp49 (1), Cp82031 (27), CPV-2a 97-003, and CPV-2b 97-008 (10) were isolated from Japanese domestic dogs. FPLV V142, CPV-2a V220, and CPV-2b V123 were isolated from domestic cats in Vietnam (16). CPV-2c(a) V139 and V140 and CPV-2c(b) V203 were isolated from Vietnamese leopard cats (Felis bengalensis) (9).…”