Isolation of HHV-6-related virus from children affected by infectious syndrome

Portolani, Marinella; Cermelli, Claudio; Pietrosemoli, Paola; Pecorari, Monica; Farabegoli, Fulvia; Cavazzuti, G. B.; Bertolani, Maria Francesca; Micheli, Amy

doi:10.1007/bf01310711

Cited by 19 publications

(5 citation statements)

References 10 publications

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“…In particular, the positive control included in the PCR assays for testing HHV-6 DNA was HHV-6 DNA extracted from the viral isolate CV obtained in the laboratory [Portolani et al, 1990] and characterized subsequently as HHV-6 variant B [Di Luca et al, 1992]. Consensus primers and PCR procedures for adenovirus and enterovirus group genomes were obtained from Morris et al [1995] and Clements et al [1995], respectively.…”

Section: Virological and Serological Studiesmentioning

confidence: 99%

“…Exanthem subitum or roseola infantum, a common benign illness, or aspeci®c febrile syndromes are the major clinical manifestations of primary HHV-6 B infections in infants [Yamanishi et al, 1988;Portolani et al, 1990], while clinical cases of febrile lymphoadenopathy have been described among the few HHV-6 seronegative adolescent and adult subjects [Steeper et al, 1989]. Central nervous system (CNS) disorders may complicate the course of HHV-6 primary infection both in infants and in adults [Kondo et al, 1993;Caserta et al, 1994;McCullers et al, 1995].…”

Section: Introductionmentioning

confidence: 99%

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Case of fatal encephalitis by HHV‐6 variant A

Portolani

Pecorari

Tamassia

et al. 2001

Journal of Medical Virology

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A fatal case is reported of encephalitis in an 85-year-old man caused by the human herpesvirus 6 variant A. The virological diagnosis was based on the ®ndings of the virus variant genomic sequences both in the cerebrospinal¯uid and serum of the patient. Moreover, virus replication in nervous tissue was suggested by a viral load higher in the cerebrospinal¯uid than in the peripheral blood. The association of a central nervous system infection with the A variant of human herpesvirus 6 is interesting because of the dif®culty in establishing a pathological role for this virus strain. Epstein-Barr virus DNA was detected in the patient's cerebrospinal¯uid in association with human herpesvirus 6 DNA. The presence of the Epstein-Barr virus genomic sequences in the cerebro-spinal¯uid was considered to be unimportant clinically.

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Section: Virological and Serological Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Case of fatal encephalitis by HHV‐6 variant A

Portolani

Pecorari

Tamassia

et al. 2001

Journal of Medical Virology

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“…The acute and convalescent sera of each patient were tested for the presence of antibodies for the following viruses: HHV-6, HHV-7, ADV, herpes simplex virus 1 (HSV-1), cytomegalovirus (CMV) and syncytial respiratory virus (SRV). With the exception of the HHV-6 and HHV-7 antigens, consisting of strains which we have isolated [21], the other antigens were supplied by commercial sources (Pasteur Diagnostics). The following immunological reactions were applied: IFA for HHV-6 and HHV-7 antibody testing, enzyme-linked immunosorbent assay (ELISA) for CMV antibodies, complement fixation test for HSV-1 antibodies and the ADV group antigen.…”

Section: Serological Studiesmentioning

confidence: 99%

A study of childhood febrile convulsions with particular reference to HHV-6 infection: pathogenic considerations

et al. 1996

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Most febrile convulsions (FC) in infants occur during a viral infection, particularly in children of less than 3 years of age; human herpesvirus 6 (HHV-6) has an important pathogenic role. To evaluate the link between this and other viruses and FC, a group of 65 children (mean age 18.46 months, SD +/- 9.19) with a first episode of simple FC (G1) was compared with 24 children (mean age 19.29 months, SD +/- 13.17) with a febrile syndrome but without FC (G2). Virological study showed the following infections: HHV-6 in 23/65 of G1 and in 12/24 of G2, adenoviruses (ADV) in 9/65 of G1 and in 0/24 of G2, syncytial respiratory virus (SRV) in 3/28 of G1 and in 0/2 of G2, HSV-1 in 6/65 of G1 and in 1/24 of G2, cytomegalovirus (CMV) in 2/65 of G1 and in 0/24 of G2 and HHV-7 in 1/42 of G1 and in 1/13 of G2. Children in G1, statistically compared with G2, were significantly more likely to have a family history of FC and circulating granulocytes, while IgM and alpha 2-globulin were less probable. Some cytokines (IL 1 beta, TNF beta and GM-CSF) were found in 24 children in G1 and 12 in G2; no differences were found between the two groups. In the light of our data and of the recent literature, the possibility that the cytokines may act on the nervous system cannot be excluded. Among the HHV-6-infected children, those suffering from convulsions were statistically more likely to have a family history of FC and IgM, while IgA were less likely. In G1, 57 cases were followed up over 2 years: 9 of them had a second episode of FC. Virological diagnosis at the first episode of FC revealed HHV-6 infection in 3 cases, 2 of these being due to viral reactivation. We underline the important role of HHV-6 infection in FC and postulate a relationship between family history and the immunity of the patient; this is confirmed by the loss of statistical significance in the reduction of IgM in G1 compared with G2 with no family history of FC. The reactivation of FC by HHV-6 is a possibility to be borne in mind; an increased number of cases would be needed to confirm this hypothesis.

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“…In this paper we derived two further MAbs to U1102 gB and examined the antigenic variations in the gB of a number of HHV-6 strains. We report that MAb 2D9 reacts specifically with GS and U1102 and does not react with Z29, the Hashimoto (Yamanishi et al, 1988) and SF (Levy et al, 1990) strains previously classified as B variants (Schirmer et al, 1991;Chandran et al, 1992), nor with CV, an as yet poorly characterized isolate derived from a roseola infantum case (Portolani et al, 1990). Our findings indicate that gB, a highly conserved glycoprotein within the Herpesviridae family, undergoes antigenic variations among the HHV-6 isolates tested.…”

mentioning

confidence: 64%

“…In addition to Ul102, the strains examined were GS, Z29, the Z29-1ike Hashimoto derived from a case of exanthem subitum (Yamanishi et al, 1988;Schirmer et al, 1991), and the B variant SF, derived in San Francisco Short communication (i) (J) (t) from the saliva of a human immunodeficiency virus-(a) positive individual (Levy et al, 1990;Chandran et al, 1 1992). The isolate CV, derived in Italy from a case of exanthem subitum (Portolani et al, 1990), was included as an as yet poorly characterized HHV-6 isolate. By IFA MAb 2D9 reacted with cells infected with the U1102 and GS but failed to react with cells infected with the Z29, Hashimoto, SF and CV isolates (Fig.…”

mentioning

confidence: 99%

Monoclonal antibodies to glycoprotein B differentiate human herpesvirus 6 into two clusters, variants A and B

Campadelli-Fiume

Guerrini

Liu

et al. 1993

Journal of General Virology

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The distribution of glycoprotein B (gB) among different human herpesvirus 6 (HHV-6) strains was analysed with a panel of three monoclonal antibodies (MAbs) derived from mice immunized with U1102-infected lymphocytes. MAb 2D9 reacted specifically by immunofluorescence and immunoprecipitation with the Ul102 and GS isolates, and failed to react with Z29 and the variant B strains Hashimoto and SF. In addition, Z29, Hashimoto and SF gB had a lower M r than U1102 and GS gB. MAb 2D9 also failed to react with the exanthem subitum isolate CV, included in this study as an as yet poorly characterized isolate. Consistent with this result, CV failed to react with the variant A-specific MAb to gp82-105 and behaved as a variant B virus even with respect to the diagnostic HindIII endonuclease restriction cleavage site located in a fragment hybridizing to the pZVHI4 probe. By contrast with MAb 2D9, MAbs 2B9 and 2D10 reacted with all of the isolates tested, strengthening the argument that they have common epitopes. Based on the antigenic and M r specificities of gB, the HHV-6 isolates tested were arranged into two non-overlapping clusters, which closely parallel the variant A and B strain groups, defined previously by several criteria, including restriction endonuclease polymorphism, antigenic variations, growth in in vitro cultures and sequence analyses.

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Isolation of HHV-6-related virus from children affected by infectious syndrome

Cited by 19 publications

References 10 publications

Case of fatal encephalitis by HHV‐6 variant A

Case of fatal encephalitis by HHV‐6 variant A

A study of childhood febrile convulsions with particular reference to HHV-6 infection: pathogenic considerations

Monoclonal antibodies to glycoprotein B differentiate human herpesvirus 6 into two clusters, variants A and B

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