2001
DOI: 10.1089/104303401450988
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Isolation of Highly Infectious and Pure Adeno-Associated Virus Type 2 Vectors with a Single-Step Gravity-Flow Column

Abstract: One of the most promising gene transfer vectors in human clinical trials is AAV2. The quality of the vector preparations is a key element in obtaining reliable and reproducible data in preclinical studies. However, established protocols either result in impure, low infectious virus (CsCl2 gradient centrifugation) or demand a high level of manual and technical skills (CsCl2 gradient centrifugation, iodixanol/heparin or HPLC purification). In this study, we present an easy-to-do single-step column purification (… Show more

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Cited by 273 publications
(213 citation statements)
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“…An alternative single-step heparin affinity purification of AAV2 by gravity flow does not require any special equipment. 21 The purity of AAV2 produced by this method is comparable to the HPLC-purified vectors. AAV2 vectors purified on heparin columns possess higher biological potency when compared to virus purified by cesium chloride density gradient, which can cause virus inactivation by aggregation.…”
Section: Purification By Affinity Chromatographymentioning
confidence: 87%
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“…An alternative single-step heparin affinity purification of AAV2 by gravity flow does not require any special equipment. 21 The purity of AAV2 produced by this method is comparable to the HPLC-purified vectors. AAV2 vectors purified on heparin columns possess higher biological potency when compared to virus purified by cesium chloride density gradient, which can cause virus inactivation by aggregation.…”
Section: Purification By Affinity Chromatographymentioning
confidence: 87%
“…Interaction of different AAV serotypes with cellular receptors has been exploited for developing rAAV purification methods by affinity chromatography. 20,21 In this manuscript, we review different purification methods that have been originally implemented in different laboratories and their utility in producing large amounts of pure and concentrated viruses.…”
Section: Adenoviruses and Adeno-associated Viruses As Vectors For In mentioning
confidence: 99%
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