“…Introduction of additional purification steps is necessary to reduce contamination with cellular proteins that bind to heparin. 20,21,50 An improved AAV2 purification method combines iodixanol density ultracentrifugation with subsequent heparin affinity purification. 50 Another method utilizing POROS HE/P (Boehringer Mannheim, Indianapolis, IN, USA) heparin affinity column introduces an additional purification of the virus eluate including trypsin digestion and extraction with an organic solvent 1,1,2-trichlorotrifluoroethane (Arklone, BDH, New York, NY, USA).…”