Isolation of Housekeeping Genes on Durik-durik (Syzygium sp)

Roslim, Dewi Indriyani; Yusfi, Liza Aulia; Hutagalung, Desriani Ritawati; Faizah, Niswah; Andriani, Lestari; Azrial, Azrial; Herman, Herman; Lestari, Wahyu

doi:10.15294/biosaintifika.v10i2.14234

Cited by 2 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…clone Riau can then be designed in both exon regions based on the obtained sequence. Such primer designing -namely P_act_F: 5'-TGG TTG GCA TGG GTC AGA A-3' and P_act_R: 5'-GTG ATG ACT TGG CCG TCT GG-3' -is aimed for certain genes ex pression studies necessity as well as a genomic 256 Volume 9 | Number 3 | September | 2019 DNA contamination verification on a target cDNA [5,6,15]. For this purpose, amplification is conducted using DNA and cDNA molecules separately.…”

Section: Resultsmentioning

confidence: 99%

“…The fresh leave total DNA isolation was conducted using DNeasy® plant mini kit (Qiagen, Canada). The total DNA was then checked on 1% agarose gel in 1× TBE buffer according to Roslim et al [15].…”

Section: Total Dna Isolationmentioning

confidence: 99%

“…The plasmids were amplified with primer pair of T3 and T7 promotors using KOD FX Neo (Toyobo, Japan) then the PCR products were purified with Zymocle-anTM Gel DNA Recovery Kit (Zymo Research, China). After that, the purified PCR products were sequenced at 1 st Base Malaysia with bi-directional sequencing [15].…”

Section: Pcr Products Cloning and Sequencingmentioning

confidence: 99%

See 2 more Smart Citations

The DNA Sequence Encoding Actin (ACT1) of Pandan (Benstonea sp.)

Wahyuningsih

Elyani²,

Damayanti³

et al. 2019

J.Trop.Life.Science

View full text Add to dashboard Cite

One of housekeeping genes is actin gene. This gene is frequently used in gene expression studies as an internal control. The DNA sequence encoding actin from Pandan (Benstonea sp.) clone Riau has not been reported, therefore, this study investigated the DNA sequence encoding actin isolated from Benstonea sp. clone Riau. Total DNA isolation was performed in fresh leaves, total RNA isolation from stem, total cDNA synthesis, polymerase chain reaction using degenerate actin primer, electrophoresis, cloning, transformation, blue white colony selection, colony PCR, sequencing, data analysis using BioEdit and MEGA6 softwares and BLASTn program. The partial DNA sequence encoding actin from Benstonea sp. clone Riau obtained was 1,403 bp. The sequence was grouped as part of actin1 (ACT1) and it was consisted of two exons and one intron. The predicted coding and peptide sequences were 616 bp and 205 amino acids, respectively. The predicted coding sequence had 90% similarity to some ACT1 mRNA from some plants species but none of which belongs to Benstonea genus or Pandanaceae family. The deduced peptide sequence had similarity to some ACT1 peptide from some plant species of up to 99% and also none of them belongs to Benstonea genus or Pandanaceae family. Thus, the partial ACT1 gene obtained in this study was the first sequence reported from Benstonea genus. Furthermore, this sequence can be used as a reference to isolate actin genes from other species within Benstonea genus for gene expression analysis purposes.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Total Dna Isolationmentioning

confidence: 99%

See 1 more Smart Citation

The DNA Sequence Encoding Actin (ACT1) of Pandan (Benstonea sp.)

Wahyuningsih

Elyani²,

Damayanti³

et al. 2019

J.Trop.Life.Science

View full text Add to dashboard Cite

show abstract

“…Procedures in this study were performed based on Roslim et al (2018). The fresh leaves were used to extract the total DNA by using Genomic DNA Mini Kit (Plant, Geneaid GP100).…”

Section: Methodsmentioning

confidence: 99%

“…Dendrogram was then constructed using MEGA6 software. These sequences analysis were conducted following a procedure suggested by Roslim et al (2018).…”

Section: Methodsmentioning

confidence: 99%

Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu)

et al. 2019

Self Cite

View full text Add to dashboard Cite

Some genes like 18S rRNA, 26S rRNA, elongation factor 1-alpha (EF1α), and beta-tubulin (TUB) are members of housekeeping genes group that are commonly used as internal control in gene expression study. This study aimed to isolate those four housekeeping genes of tuntun angin (Elaeocarpus floribundus). The research material included fresh leaves of E. floribundus that were picked up from Kajuik Lake in Riau Province and four primer pairs. The procedures consisted of total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), polymerase chain reaction (PCR), electrophoresis on 1% agarose gel, sequencing, and bioinformatic analysis. This study has been isolated 18S rRNA, 26S rRNA, EF1α, and TUB genes with the size of 422 bp, 922 bp, 856 bp, and 877 bp, respectively. The EF1α and TUB genes has never been reported in Elaeocarpaceae family. Thus, those partial DNA sequences are the first sequences reported from this species and can be used as reference genes in this plant after validation.

show abstract

Isolation of Housekeeping Genes on Durik-durik (Syzygium sp)

Cited by 2 publications

References 30 publications

The DNA Sequence Encoding Actin (ACT1) of Pandan (Benstonea sp.)

The DNA Sequence Encoding Actin (ACT1) of Pandan (Benstonea sp.)

Isolation of Partial Housekeeping Genes on Tuntun Angin (Elaeocarpus floribundu)

Contact Info

Product

Resources

About