Isolation of human explant derived cardiac stem cells from cryopreserved heart tissue

Abstract

The value of preserving high quality bio specimens for fundamental research is significant as linking cellular and molecular changes to clinical and epidemiological data has fueled many recent advances in medicine. Unfortunately, storage of traditional biospecimens is limited to fixed samples or isolated genetic material. Here, we report the effect of cryopreservation of routine myocardial biopsies on explant derived cardiac stem cell (EDC) culture outcomes. We demonstrate that immediate cryopreservation or delayed cryopreservation after suspension within cardioplegia for 12 hours did not alter EDC yields, proliferative capacity, antigenic phenotype or paracrine signature. Cryopreservation had negligible effects on the ability of EDCs to adopt a cardiac lineage, stimulate new vessel growth, attract circulating angiogenic cells and repair injured myocardium. Finally, cryopreservation did not influence the ability of EDCs to undergo genetic reprogramming into inducible pluripotent stem cells. This study establishes a means of storing cardiac samples as a retrievable live cell source for cardiac repair or disease modeling.

Glyoxalase 1 Prevents Chronic Hyperglycemia Induced Heart-Explant Derived Cell Dysfunction

“…Finally, panning through ventricular lysate failed to demonstrate meaningful persistence of transplanted cells. Previous work has shown that long-term persistence of transplanted cells is very modest (1-2% of the initial injectate) 19, 20, 22, 23, 50. It is possible the number of cells remaining was below the detection threshold for the male-specific target used 28 but this result needs to be verified and future work to define the critical retention window leading to cell treatment effects needs to be established.…”

Human Heart Explant-Derived Extracellular Vesicles: Characterization and Effects on the In Vitro Recellularization of Decellularized Heart Valves

“…A wide variety of cell types, such as cardiomyocytes, endothelial cells, smooth muscle cells and cardiac resident stromal cells (CRSCs), such as fibroblasts and stem/progenitor cells, are found in the adult heart [1,2]. CRSCs have been isolated and characterized by the ex vivo culture of cardiac explants [3,4,5]. The conditioned media from cultured CRSCs exhibits cardioprotective and regenerative capabilities, being able to drive proliferation, cardiac differentiation and tube formation in endothelial cells [4].…”

Cardiac differentiation of bone marrow resident c-kit+ stem cells by L-carnitine increases through secretion of VEGF, IL6, IGF-1 and TGF-β

“…Recently, stem cells usage has been known as an attractive method, which could regenerate and repair the lost heart organ [6]. Nowadays, broadly used cells in the animal modeling are modeling that are somatic stem cells from various adult tissues, embryonic stem (ES) cells, and induced pluripotent stem (iPS) cells [7,8]. As mentioned earlier, somatic stem cells-derived from various tissues such as adipose tissue, bone marrow (BM), and heart have been used to regenerate dead myocardium; however, its efficiency is low due to progressive loss of functional role and reduced output [9].…”