Isolation of Hyperoside and Isoquercitrin from Camellia sasanqua as Antioxidant Agents

Sukito, Agus; Tachibana, Sanro

doi:10.3923/pjbs.2014.999.1006

Cited by 35 publications

(12 citation statements)

References 20 publications

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“…However, the hexane extract from S. tuberosa leaves showed higher DPPH scavenging activity than extracts of Spondias pinnata , which showed IC 50 ranging from 0.73 to 0.59 mg/mL [ 33 ]. The hyperoside possesses DPPH scavenging activity with an IC 50 of 27.5 mM [ 34 ] and polyunsaturated fatty acids may act as antioxidants [ 35 ]. Thus, flavonoids and fatty acids may be involved in the antioxidant property of the hexane extract of S. tuberosa leaves.…”

Section: Discussionmentioning

confidence: 99%

Hexane extract from Spondias tuberosa (Anacardiaceae) leaves has antioxidant activity and is an anti-Candida agent by causing mitochondrial and lysosomal damages

Cordeiro

Santos

Ferreira

et al. 2018

BMC Complement Altern Med

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BackgroundSpondias tuberosa is a plant that produces a fruit crop with high economic relevance at Brazilian Caatinga. Its roots and leaves are used in folk medicine.MethodsChemical composition of a hexane extract from S. tuberosa leaves was evaluated by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and 1H nuclear magnetic resonance (NMR). Antioxidant potential was investigated by DPPH and ABTS assays. Antifungal action on Candida species was evaluated determining the minimal inhibitory concentration (MIC50) and putative mechanisms were determined by flow cytometry analysis. In addition, hemolytic activity on human erythrocytes was assessed and the concentration required to promote 50% hemolysis (EC50) was determined.ResultsPhytochemical analysis by TLC showed the presence of flavonoids, hydrolysable tannins, saponins and terpenes. The HPLC profile of the extract suggested the presence of gallic acid (0.28 ± 0.01 g%) and hyperoside (1.27 ± 0.01 g%). The representative 1H NMR spectrum showed saturated and unsaturated fatty acids among the main components. The extract showed weak and moderate antioxidant activity in DPPH (IC50: 234.00 μg/mL) and ABTS (IC50: 123.33 μg/mL) assays, respectively. It was able to inhibit the growth of C. albicans and C. glabrata with MIC50 of 2.0 and 0.078 mg/mL, respectively. The treatment of C. glabrata cells with the extract increased levels of mitochondrial superoxide anion, caused hyperpolarization of mitochondrial membrane, and compromised the lysosomal membrane. Weak hemolytic activity (EC50: 740.8 μg/mL) was detected.ConclusionThe results demonstrate the pharmacological potential of the extract as antioxidant and antifungal agent, aggregating biotechnological value to this plant and stimulating its conservation.

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Section: Discussionmentioning

confidence: 99%

Hexane extract from Spondias tuberosa (Anacardiaceae) leaves has antioxidant activity and is an anti-Candida agent by causing mitochondrial and lysosomal damages

Cordeiro

Santos

Ferreira

et al. 2018

BMC Complement Altern Med

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“…Some studies have indicated that hyperoside is the major flavonoid of Z. bungeanum leaves ( 12 – 15 ). Pharmacological investigations have demonstrated that hyperoside have diverse biological activities such as antioxidant ( 12 , 16 ), anticancer ( 17 ), anti-inflammatory ( 18 ), anticoagulant ( 19 ) and cardioprotective activities ( 20 ). Moreover, hyperoside has been found to play crucial roles in rat lens aldose reductase inhibition ( 21 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes

et al. 2017

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The development of diabetes mellitus (DM) is accompanied by hyperglycemia-induced oxidative stress. Hyperoside is a major bioactive component in Zanthoxylum bungeanum leaves (HZL) and is a natural antioxidant. However, the effects of HZL on DM and its mechanisms of action remain undefined. The present study evaluated the anti-hypoglycemic and hepatocyte-protective effects of HZL in mice with diabetes induced by a high-carbohydrate/high-fat diet (HFD) and alloxan. We also aimed to eludicate the underlying mechanisms. Our resutls demonstrated that the administration of HZL significantly reduced body weight gain, serum glucose levels and insulin levels in diabetic mice compared with the vehicle-treated mice. In addition, the levels of dyslipidemia markers including total cholesterol, triglyceride and low-density lipoprotein cholesterol in the HFD-treated mice were markedly decreased. Further experiments using hepatocytes from mice revealed that HZL significantly attenuated liver injury associated with DM compared with vehicle treatment, as evidenced by lower levels of alanine aminotransferase and aspartate aminotransferase in serum and by lower levels of lipid peroxidation, nitric oxide content and inducible nitric oxide synthase activity in liver tissues. Nuclear factor-κB (NF-κB) and mitogen-associated protein kinase (MAPK) signaling pathways were investigated to elucidate the molecular mechanisms responsible for the protective effects of HZL against diabetic liver injury. The results indicated that HZL inhibited the phosphorylation of p65/NF-κB, MAPK (including p38, JNK and ERK1/2) and activating transcription factor 3 protein expression, with an additional suppression of Bax, cytochrome c, caspase-9 and caspase-3 in the liver tissues of diabetic mice. Taken together, our findings suggest that HZL, which was effective in inhibiting oxidative stress-related pathways may be beneficial for use in the treatment of DM.

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“…Based on these results, the F-EtOAc leaf fraction was separated by column chromatography to obtain a flavonoid glycoside, isoquercitrin (7.3 mg); the structure is shown in Figure 2. The structural characterization of this compound was carried out by spectroscopic and spectrometric analyses and by comparison with published data 26,27 .…”

Section: Resultsmentioning

confidence: 99%

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

Silva-Rivas¹,

Bailón-Moscoso²,

Cartuche³

et al. 2020

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Introduction: The prevalence of diabetes has increased more rapidly in low and middleincome countries than in high-income countries. Type 2 diabetes mellitus (DM2), which is the most common form of diabetes, is caused by the inefficient use of insulin in the body and is characterized by disrupted insulin action or secretion. Also, oxidative stress plays an important role in the development of disease. The goal of this study is to identify the antioxidant and hypoglycemic properties of Clusia latipes, an endemic species of Central and South America. Methods: The antioxidant and hypoglycemic capacity of the extracts (hexane, ethyl acetate, and methanol) of the leaves and stems of Clusia latipes were evaluated. From the most potent extract, the phytochemical study was carried out and fractionated. Antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), while hypoglycemic capacity was measured by alpha-glucosidase inhibition. Results: The extracts with the highest antioxidant capacity are the extracts with the highest α-glucosidase inhibition activity. Inhibitory activity increased in samples extracted with medium polar (ethyl acetate) and polar (methanol) solvents. Phytochemical screening of these extracts revealed the presence of alkaloids, carbohydrates, flavonoids/xanthones, quinones, saponins, and tannins. The highest α-glucosidase inhibitory activity was detected in the ethyl acetate fraction obtained from leaf methanol extract, with a half-maximal inhibitory concentration (IC 50 ) value of 0.90 μg/ml. The major constituent isolated from the same fraction was isoquercitrin.

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Isolation of Hyperoside and Isoquercitrin from Camellia sasanqua as Antioxidant Agents

Cited by 35 publications

References 20 publications

Hexane extract from Spondias tuberosa (Anacardiaceae) leaves has antioxidant activity and is an anti-Candida agent by causing mitochondrial and lysosomal damages

Hexane extract from Spondias tuberosa (Anacardiaceae) leaves has antioxidant activity and is an anti-Candida agent by causing mitochondrial and lysosomal damages

Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

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