Isolation of<i>Agrobacterium tumefaciens</i>Biovar 3 from Grapevine Galls and Sap, and from Vineyard Soil

Burr, Thomas J.; Katz, Barbara H.

doi:10.1094/phyto-73-163

Cited by 73 publications

(45 citation statements)

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“…Samples from all replications of each treatment were pooled and mixed thoroughly, and subsamples were taken for microbiological analysis. Soil was serially diluted and transferred to New & Kerr (1971) modified selective medium (Burr & Katz, 1983). Soil sampling was done before and immediately after the treatment, and periodically during the following year to monitor the population dynamics of agrobacteria.…”

Section: Methodsmentioning

confidence: 99%

The effect of solar heating of soil on natural and inoculated agrobacteria

Raio¹,

Zoina

Moore

1997

Plant Pathology

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Solarization trials were carried out over 3 years and in two countries to control crown gall disease on fruit trees and eliminate Agrobacterium. In 1992, agrobacteria in naturally infested soils of two Italian nurseries were monitored before and after solarization. Agrobacteria populations decreased by 99% and 92% after the treatment; however, crown gall incidence did not decrease. In 1993 and 1994 solarization was tested in Oregon in fields artificially infested with two marked strains of A. tumefaciens. In sandy loam soil, the target bacteria were eliminated in 4 weeks, while in silty clay soil the populations were markedly reduced after 2 months of treatment. Crown gall incidence on cherry rootstocks transplanted to the field at the end of 1993 was 3·7% in the sandy loam soil control plots, while no tumours were observed on plants from solarized plots. The use of solarization in combination with reduced doses of metham-sodium was also evaluated.

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Section: Methodsmentioning

confidence: 99%

The effect of solar heating of soil on natural and inoculated agrobacteria

Raio¹,

Zoina

Moore

1997

Plant Pathology

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“…It is possible that the presence of the DNA A in the upper leaves results from spread and subsequent reinfection by A. tumefaciens within the vascular system, as has been reported to occur in grapevine (Burr & Katz, 1983) and the legume Sesbania rostrata (Vlachova et al, 1987). Using stem segments, leaf discs, leaf veins and extracts thereof, we have been unable to recover A. tumefaciens on precallusing plates (Horsch & Klee, 1986) or L agar plates with or without kanamycin selection (Klinkenberg et al, 1989) as was accomplished by Vlachova et al (1987).…”

mentioning

confidence: 92%

Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana

Klinkenberg

Stanley

1990

Journal of General Virology

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“…All but one were identified as tumorigenic strains of Ag. vitis by traditional microbiological (Burr and Katz 1983;Paulus et al 1989a) and molecular methods (Otten et al 1996a). …”

Section: Bacterial Strainsmentioning

confidence: 99%

Genetic diversity of Agrobacterium vitis as determined by DNA fingerprints of the 5′‐end of the 23S rRNA gene and Random Amplified Polymorphic DNA

Momol

Burr

Reid

et al. 1998

Journal of Applied Microbiology

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E .A . M O MO L, T .J . B U RR , C . L. RE I D, M. T . M OM O L, S. H . H SE U AN D L . OT TE N . 1998. Sixty-nine strains of Agrobacterium vitis, the causal agent of grape crown gall, originating from different geographical regions of the USA and Europe, were characterized by fingerprint analysis of the 5?-end of the 23S rRNA gene and by Random Amplified Polymorphic DNA (RAPD) markers. For 5?-end 23S fingerprinting, amplicons were digested with TaqI, RsaI, AvaI, CfoI and AluI. For RAPD analysis, three 10-mer primers were used to generate PCR products. There was a high degree of correlation between strain groupings generated by the two methods. However, more diversity was identified when groupings were based on RAPDs. For example, 28 of 29 strains having nopaline type Ti plasmids generated identical 5?-end 23S patterns but formed two distinct RAPD groups that separated strains originating from the USA and Hungary. Similarly by RAPDs, one cluster of strains carrying vitopine-type Ti plasmids could be separated into those originating in the USA and Europe. The composition of strain groups generated by 5?-end 23S and RAPDs were highly correlated with a previous fingerprint analysis of the intergenic spacer region (located between the 16S and 23S rRNA genes) and with RFLP analysis for characterizing Ti plasmids. These findings show that among Ag. vitis strains there is a high level of correlation between two regions of the rRNA operon, total genomic DNA (as determined by RAPDs) and the type of Ti plasmid they carry. INTRODUCTIONCrown gall caused by Agrobacterium vitis (Ophel and Kerr 1990) is a serious disease of grapes worldwide, particularly on cultivars of Vitis vinifera. The bacterium survives systemically in vines and in addition to tumours, it causes tissue necrosis that is associated with production of a chromosomally-encoded polygalacturonase (McGuire et al. 1991;Rodriguez-Palenzuela et al. 1991;Herlache et al. 1997). Tumours usually develop on the lower trunks of vines near graft unions. Grapevines carrying Ag. vitis systemically may form galls when chemical signals from wounded plants induce the infection process by the pathogen (Burr et al. 1987). Tumorigenic and non-tumorigenic strains of Ag. vitis coexist in galls and symptomless tissues and may be disseminated in propagation material Katz 1983, 1984).Correspondence to : Dr E. A. Momol, Department of Plant Pathology, Cornell University, Geneva, NY 14456, USA (e-mail: eam17@cornell.edu). © 1998 The Society for Applied MicrobiologyTumorigenic strains of Ag. vitis have been previously characterized based on the types of opine synthesis and catabolic genes they carry on their Ti plasmids. Important characteristics of Ti plasmids are the genetic make-up of the T-DNA that is transferred and expressed in plants, the virulence genes that are responsible for packaging and transport of T-DNA, and the genes coding for opine synthesis and catabolism. Ti plasmids differ structurally and are generally divided into those that encode the synthesis of nopaline (contai...

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Isolation ofAgrobacterium tumefaciensBiovar 3 from Grapevine Galls and Sap, and from Vineyard Soil

Cited by 73 publications

References 0 publications

The effect of solar heating of soil on natural and inoculated agrobacteria

The effect of solar heating of soil on natural and inoculated agrobacteria

Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana

Genetic diversity of Agrobacterium vitis as determined by DNA fingerprints of the 5′‐end of the 23S rRNA gene and Random Amplified Polymorphic DNA

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