Isolation of <i>Brucella abortus</i> from cattle and water buffalo in Brazil

Megid, Jane; Albert, Daniel M.; Fagliari, J. J.; Paes, Antônio Carlos; Listoni, Fernando Paganini; Pinto, Mary Beth; Ribeiro, Márcio Garcia; Thiébaud, M.; Ueno, Takako; Garin‐Bastuji, Bruno

doi:10.1136/vr.156.5.147

Cited by 18 publications

(13 citation statements)

References 5 publications

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“…B. abortus bv1 was the most frequent biovar isolated, as also described by previous studies in Brazil [3], [12], [33] and most regions of the world [4], [18]. Interestingly, our B. abortus bv2 isolates did not need addition of serum to culture medium for their isolation, as it is usually described for strains of this biovar [27].…”

Section: Discussionsupporting

confidence: 79%

“…Interestingly, our B. abortus bv2 isolates did not need addition of serum to culture medium for their isolation, as it is usually described for strains of this biovar [27]. This phenomenon was also observed for other B. abortus bv2 strains from Brazil [12]. Of the two biovars that were described for the first time in Brazil (bv4 and bv6), only one strain of B. abortus bv4 was isolated from Rio Grande do Sul.…”

Section: Discussionsupporting

confidence: 73%

“…in Brazil describing B. abortus bv1, 2 and 3 and B. suis bv1 [9], [11], [12]. Also, B. canis and B. ovis were found infecting domestic animals [3].…”

Section: Introductionmentioning

confidence: 99%

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Biotyping and Genotyping (MLVA16) of Brucella abortus Isolated from Cattle in Brazil, 1977 to 2008

et al. 2013

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Brucellosis is a worldwide distributed zoonosis that causes important economic losses to animal production. In Brazil, information on the distribution of biovars and genotypes of Brucella spp. is scarce or unavailable. This study aimed (i) to biotype and genotype 137 Brazilian cattle isolates (from 1977 to 2008) of B. abortus and (ii) to analyze their distribution. B. abortus biovars 1, 2 and 3 (subgroup 3b) were confirmed and biovars 4 and 6 were first described in Brazil. Genotyping by the panel 1 revealed two groups, one clustering around genotype 40 and another around genotype 28. Panels 2A and 2B disclosed a high diversity among Brazilian B. abortus strains. Eighty-nine genotypes were found by MLVA16. MLVA16 panel 1 and 2 showed geographic clustering of some genotypes. Biotyping and MLVA16 genotyping of Brazilian B. abortus isolates were useful to better understand the epidemiology of bovine brucellosis in the region.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionsupporting

confidence: 73%

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Biotyping and Genotyping (MLVA16) of Brucella abortus Isolated from Cattle in Brazil, 1977 to 2008

et al. 2013

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“…The main pathological findings were fibrinous suppurative necrotic placentitis, and chronic endometritis. te para bubalinos, assim como para bovinos, é uma bactéria intracelular facultativa, a Brucella abortus biotipo1 (Cheville et al 1993, Fosgate et al 2002, Megid et al 2005), que tem como principal fonte de contaminação a via digestiva (Payne 1959). A brucelose provoca grandes prejuízos econômicos devido a problemas reprodutivos (Corbel 1997) e é uma das zoonoses que também afeta o búfalo doméstico.…”

Section: Introductionunclassified

Infecção transplacentária e intrauterina por Brucella abortus em búfalos (Bubalus bubalis)

et al. 2015

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RESUMO: O objetivo deste trabalho foi verificar a presença de Brucella abortus e as lesões causadas por esse agente nos anexos fetais e nos fetos de búfalas. Para isso, 20 búfalas em diversos meses de gestação, sorologicamente positivas para brucelose, foram submetidas ao abate sanitário. A idade fetal foi determinada através de exames ultrassonográficos associados à mensuração dos fetos durante a necropsia. Do útero fechado desses animais foram coletadas amostras para histopatologia e qPCR. A partir do segundo mês de gestação foi possível detectar a presença de DNA de B. abortus em líquido amniótico, líquido alantoide e em útero e, a partir do quinto mês, na placenta, coração, baço, rim, pulmão, intestino, fígado e linfonodos dos fetos. Os principais achados anatomopatológicos foram placentite fibrinopurulenta necrótica e endometrite supurativa crônica.

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“…A enfermidade é caracterizada por ser uma zoonose bacteriana, causada por Brucella abortus, com importância econômica devido a problemas reprodutivos (Corbel 1997), sendo o principal agente para bubalinos B. abortus biotipo1 (Fosgate et al 2002, Megid et al 2005. As vias de infecção por B. abortus podem ser alimentar, genital ou respiratória e as fontes principais são fetos abortados, restos placentários, exsudatos uterinos ou Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação 1 sêmen contaminado (Payne 1959, Schlafer & Miller 2007.…”

Section: Introductionunclassified

Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação

et al. 2015

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The objective of this study was to detect Brucella abortus in lymph nodes of buffaloes as well as to describe the lesions caused. Nineteen buffalo cows in various stages of pregnancy, serologically positive for brucellosis and subjected to culling were used. Fetal age was determined by ultrasound examination and the size of fetuses was measured at necropsy. Fragments of lymph nodes were collected for histopathology and qPCR. The detection of B. abortus DNA in the lymph nodes was checked from the fourth month of pregnancy in seven buffaloes and in a post-calving buffalo. Acute to chronic lymphadenitis was histologically diagnosed. B. abortus DNA was detected in all evaluated groups of lymph nodes; the mammary lymph nodes were the most affected.

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Isolation of Brucella abortus from cattle and water buffalo in Brazil

Cited by 18 publications

References 5 publications

Biotyping and Genotyping (MLVA16) of Brucella abortus Isolated from Cattle in Brazil, 1977 to 2008

Biotyping and Genotyping (MLVA16) of Brucella abortus Isolated from Cattle in Brazil, 1977 to 2008

Infecção transplacentária e intrauterina por Brucella abortus em búfalos (Bubalus bubalis)

Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação

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