Isolation of<i>Elaeagnus</i>-compatible<i>Frankia</i>from soils collected in Tunisia

Gtari, Maher; Brusetti, L.; Skander, Gharbi; Mora, Diego; Boudabous, Abdellatif; Daffonchio, D.

doi:10.1111/j.1574-6968.2004.tb09554.x

Cited by 49 publications

(15 citation statements)

References 28 publications

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“…Frankia cluster 2 dispersal outward from the Coriaria myrtifolia rhizosphere While the presence of Frankia cluster 2 (the host compatible microsymbionts) and cluster 4 (asymbiotic Frankia that are often isolated from Coriaria nodules; Mirza et al, 1992;Gueddou et al, 2017) was expected, the occurrence of cluster 1 and the superabundance of cluster 3 in C. myrtifolia nodule, root, and rhizosphere was surprising. As previously stated, clusters 1 and 3 are known to ubiquitously distributed in soils independent to the presence of compatible host-plants (Paschke and Dawson, 1992;Nalin et al, 1997;Gtari et al, 2004). Cluster 4 is also known to be found free-living in soil (Wolters et al, 1997).…”

Section: Rhizocompartment Microbiomesmentioning

confidence: 75%

“…Isolation of many Frankia strains in pure culture is strong indirect evidence of the ability of strains to thrive asymbiotically in soils. Infective units from clusters 1, 3 and 4 have been shown to be ubiquitously distributed in soils around the world, (Paschke and Dawson, 1992;Nalin et al, 1997;Gtari et al, 2004) even in newly deposited glacial till, volcanic lava deposits, and young sand dunes prior to colonization by host plants (Young et al, 1992). Moreover, Frankia strains from cluster 1 were found to occur abundantly in non-host rhizosphere (Smolander, 1990) and fresh lava deposit with the volcanophile actinorhizal plant species Myrica and Casuarina (Burleigh and Dawson, 1994;Yamanaka and Okabe, 2006;Kurten et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Decrypting phytomicrobiome of the neurotoxic actinorhizal species, Coriaria myrtifolia, and dispersal boundary of Frankia cluster 2 in soil outward compatible host rhizosphere

Swanson

Sbissi²,

Ktari³

et al. 2022

Front. Microbiol.

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The actinorhizal plant, Coriaria myrtifolia, is a neurotoxic plant species endemic to the western Mediterranean area, which forms a nitrogen-fixing symbiosis with members of Frankia cluster 2. Contrarily to other Frankia clusters, the occurrence and mode of dispersal for infective cluster 2 units outside of the host plant rhizosphere remains controversial. The present study was designed to investigate the structure of the microbiomes of C. myrtifolia phytosphere, rhizosphere, and soil samples extending outward linearly up to 1 km. Results showed that the epiphyte and endophyte communities were not significantly different from each other for most of the plant tissues. The communities associated with the below-ground tissues (nodule and root) were significantly different from those found on the above-ground tissues (fruit, leaves, and stems) and had a higher community richness. Coriaria myrtifolia phytomicrobiomes were dominated by Cyanobacteria for leaf, stem, and fruit while Actinobacteria and Proteobacteria were dominant in the root and nodule organelles. The nodule, a special niche for nitrogen fixation, was mainly inhabited by Frankia but contained several non-Frankia bacteria. Beside Frankia cluster 2, the presence of clusters 1, 4, and large numbers of cluster 3 strains have been detected in nodules, roots, and rhizospheres of C. myrtifolia. Despite Frankia being found in all plots using plant trapping bioassays with C. myrtifolia seedlings, Frankia cluster 2 was not detected in soil metagenomes showing the limits of detection by this approach. This result also suggests that in the absence of appropriate host plant species, Frankia cluster 2 has a reduced number of infective units present in the soil outward from the rhizosphere.

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Section: Rhizocompartment Microbiomesmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Decrypting phytomicrobiome of the neurotoxic actinorhizal species, Coriaria myrtifolia, and dispersal boundary of Frankia cluster 2 in soil outward compatible host rhizosphere

Swanson

Sbissi²,

Ktari³

et al. 2022

Front. Microbiol.

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“…DNA extraction from root nodules was performed as described by Gtari et al 20 . Individual lobe from the 44 root nodules were surface sterilized as described for microsymbiont isolation protocol, ground in nitrogen liquid and taken up in the extracting buffer (100 mmol l −1 Tris-HCl [pH 8]; 20 mmol l −1 EDTA [pH8.2]; 1.4 M NaCl, and 2% w/v cetyl trimethyl ammonium bromide; CTAB).…”

Section: Methodsmentioning

confidence: 99%

“…35,36 Alongside with the asymbiotic Frankia strains, the protocol used in the present study permitted the isolation of several other actinobacteria which were affiliated to Micrococcus, Micromonospora, Nocardia, Plantactinospora, and Streptomyces genera. Numerous are the studies which reported the isolation of non-Frankia actinobacteria from actinorhizal root nodules 18,20,34,[37][38][39][40][41][42][43][44][45] as it was shown before for legume nodules which contain both rhizobial and non-rhizobial bacteria. 46,47 Three type strains; Micromonospora coriariae 42 ; Nocardia casuarinae 44 and Nocardia alni 48 have been isolated respectively from Coriaria; Casuarina and Alnus root nodules.…”

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confidence: 99%

Root Nodule Microsymbionts of Native Coriaria myrtifolia in Algeria

et al. 2022

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Coriaria myrtifolia occurs as natural flora of warm temperate climates of northern Algeria which commonly found in hedges, forest and ravine edges. This actinorhizal species was known to establish a mutualistic symbiosis with members of phylogenetic cluster 2 (including strains associated to Coriaria spp., Ceanothus, Datiscaceae, and Dryadoideae) within the genus Frankia. Attempts to isolate C. myrtifolia microsymbionts from native plants growing in 4 locations in Algeria permitted to only recover asymbiotic Frankia strains (unable to reestablish nodulation and to fix nitrogen) from phylogenetic cluster 4 and several non- Frankia actinobacteria including members of Micrococcus, Micromonospora, Nocardia, Plantactinospora, and Streptomyces genera. The biodiversity of Frankia microsymbionts of C. myrtifolia root nodules was assessed using PCR-amplification followed by partial nucleotide sequencing of glnA1 (glutamine synthetase type 1) gene. On the 12 different glnA1 gene sequences obtained in this study, 9 were detected for the first time, and were mainly closelyrelated to Mediterranean genotypes previously described in the Grand Maghreb countries (Morocco and Tunisia) and in Europe (France) but without clear separations from other cluster 2 genotypes.

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“…Furthermore, another study revealed that the alliance of arbuscular mycorrhizal (Glomus mosseae) and rhizobial (R. leguminosarum) symbioses alleviated damage to clover (Trifolium repens) by root hemiparasitic Pedicularis species [42]. The N2-fixing Frankia, one genus belonging to the actinobacteria group, form the actinorhizal nodules with actinorhizal plants, and promote the growth and abiotic resistance for several woody plants, such as Alnus rubra, Hippophae tibetana, Elaeagnus angustifolia, and Casuarina glauca [30,[43][44][45][46]. Root nodule formation in plants by Frankia spp.…”

Section: Plant Growth-promoting Bacteriamentioning

confidence: 99%

Research Advances of Beneficial Microbiota Associated with Crop Plants

Tian

Lin

Tian

et al. 2020

IJMS

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Plants are associated with hundreds of thousands of microbes that are present outside on the surfaces or colonizing inside plant organs, such as leaves and roots. Plant-associated microbiota plays a vital role in regulating various biological processes and affects a wide range of traits involved in plant growth and development, as well as plant responses to adverse environmental conditions. An increasing number of studies have illustrated the important role of microbiota in crop plant growth and environmental stress resistance, which overall assists agricultural sustainability. Beneficial bacteria and fungi have been isolated and applied, which show potential applications in the improvement of agricultural technologies, as well as plant growth promotion and stress resistance, which all lead to enhanced crop yields. The symbioses of arbuscular mycorrhizal fungi, rhizobia and Frankia species with their host plants have been intensively studied to provide mechanistic insights into the mutual beneficial relationship of plant–microbe interactions. With the advances in second generation sequencing and omic technologies, a number of important mechanisms underlying plant–microbe interactions have been unraveled. However, the associations of microbes with their host plants are more complicated than expected, and many questions remain without proper answers. These include the influence of microbiota on the allelochemical effect caused by one plant upon another via the production of chemical compounds, or how the monoculture of crops influences their rhizosphere microbial community and diversity, which in turn affects the crop growth and responses to environmental stresses. In this review, first, we systematically illustrate the impacts of beneficial microbiota, particularly beneficial bacteria and fungi on crop plant growth and development and, then, discuss the correlations between the beneficial microbiota and their host plants. Finally, we provide some perspectives for future studies on plant–microbe interactions.

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Isolation ofElaeagnus-compatibleFrankiafrom soils collected in Tunisia

Cited by 49 publications

References 28 publications

Decrypting phytomicrobiome of the neurotoxic actinorhizal species, Coriaria myrtifolia, and dispersal boundary of Frankia cluster 2 in soil outward compatible host rhizosphere

Decrypting phytomicrobiome of the neurotoxic actinorhizal species, Coriaria myrtifolia, and dispersal boundary of Frankia cluster 2 in soil outward compatible host rhizosphere

Root Nodule Microsymbionts of Native Coriaria myrtifolia in Algeria

Research Advances of Beneficial Microbiota Associated with Crop Plants

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