Isolation of infectious C-type oncornavirus from human leukaemic bone marrow cells

Cultured peripheral blood leukocytes from a woman (patient HL23) with acute myelogenous leukemia produced type-C RNA tumor viruses (HL23V). The viruses were analyzed by molecular hybridization experiments after transmission to five secondary cell culture lines. Using the criteria of molecular hybridization, we concluded that al of the transmitted virus isolates have nucleotide sequences related to the genome of simian sarcoma virus (SiSV). In addition, in agreement with data reported elsewhere, some of the transmitted viruses also have nucleotide sequences related to those of the baboon endogenous virus (BaEV).We also used molecular hybridization to ascertain whether both viruses could have originated from the patient HL23.Utilizing [3H]cDNA complementary to RNA from the separated

mentioning

confidence: 99%

Primate type-C virus nucleic acid sequences (woolly monkey and baboon types) in tissues from a patient with acute myelogenous leukemia and in viruses isolated from cultured cells of the same patient.

Reitz¹,

Miller²,

Wong‐Staal³

et al. 1976

“…On the other hand, two reports document a failure to find such antibodies (19,20). The recent isolates (1)(2)(3)(4)(5) of replicating virus from human tissues proved to be closely related to the simian sarcoma-gibbon ape lymphoma virus group or to the endogenous baboon viruses. We have therefore used the simian sarcoma-associated helper virus (SSAV) (21) and the M7 strain of baboon endogenous virus (BEV) (22) to survey human sera for specific antibodies.…”

mentioning

confidence: 99%

“…A number of reports describe the isolation of infectious, replicating type-C viruses from human tissues (1)(2)(3)(4)(5). There have also been reports on the presence of antigens in tissues of normal individuals as well as of patients with neoplastic or autoimmune diseases that react with antisera specific to structural proteins of mammalian type-C viruses (6)(7)(8)(9).…”

mentioning

confidence: 99%

Natural human antibodies reactive with primate type-C viral antigens.

Kurth¹,

Teich²,

Weiss³

et al. 1977

A survey of human sera from healthy individuals revealed the presence of naturally occurring antibodies that react in radioimmunoprecipitation assays with proteins of mammalian type-C viruses. Of 39 sera tested, 100% showed reactivity against baboon endogenous virus, whereas only 49% showed reactivity-against simian sarcoma-associated virus. Polyacrylamide gel electrophoresis of immune precipitates revealed one to three bands that comigrate with the virus structural proteins. There were low, but detectable, levels of antibody to the major internal protein of murine leukemia virus, but no activity against the structural proteins of avian sarcoma virus.

“…Sci. USA 74 (1977) viruses highly related to mem'bers of this virus group (25)(26)(27)(28)(29)(30) and, more recently, suggestive evidence for antibodies to this virus group in human sera (19,39,40). However, proviral sequences related to the simian sarcoma virus/gibbon ape leukemia virus group have not as yet been reported (14).…”

mentioning

confidence: 99%

“…Recently, nucleic acids and proteins related to the two known primate type-C virus groups-the simian sarcoma virus/gibbon ape leukemia virus group and the baboon endogenous virus group-have been reported in fresh human tissues (5-8, 11-14, 16, 18-23) (20)(21)(22)(23) have been contested (24). Although clearly a rare event, isolation of a replicating virus from human tissues related to one or both of these primate type-C viruses has also been reported (25)(26)(27)(28)(29)(30). Until The objective of experiments reported here was to examine DNA from human tissues for sequences related to RNA of MuLVR in more detail and with more carefully prepared cDNA probes.…”

mentioning

confidence: 99%

Rauscher-leukemia-virus-related sequences in human DNA: presence in some tissues of some patients with hemotopoietic neoplasias and absence in DNA from other tissues.

Aulakh¹,

Gallo²

1977

Nature 262,[190][191][192][193][194][195], because there is no sequence homology between nucleic acids from MuLVR and baboon virus. The absence of these nucleic acid sequences in many tissues of patients with neoplasia and from the few tissues examined from people with nonneoplastic disease suggests that they are not endogenous elements but are acquired after fertilization. Taken together with the previous detection of baboon and woolly monkey type-C viral related components in some human tumors, the results suggest acquisition of at least three types of type-C viral sequences in the human population. Data are accumulating that indicate the presence of type-Ctumor-virus-like components in some human tissues (1-23). Cytoplasmic nucleic acids with sequences related to the RNA of Rauscher murine leukemia virus (MuLVR) and other viruses have been described (4, 9-17). Recently, nucleic acids and proteins related to the two known primate type-C virus groups-the simian sarcoma virus/gibbon ape leukemia virus group and the baboon endogenous virus group-have been reported in fresh human tissues (5-8, 11-14, 16, 18-23) The objective of experiments reported here was to examine DNA from human tissues for sequences related to RNA of MuLVR in more detail and with more carefully prepared cDNA probes. We report: (i) detection of nucleic acid sequences distantly related to MuLVR in DNA from spleens of some patients with acute myelogenous leukemia (AML), Hodgkin's disease, and multiple myeloma; (ii) absence of these sequences in DNA from apparently uninvolved tissues of the same patients with neoplasia and from the normal human tissues tested. Since MuLVR and the baboon endogenous virus are not detectably related (32), the detection of MuLVR-related DNA sequences is not explained by the previously reported presence of proviral sequences of baboon virus in DNA from some leukemia patients. Taken together, the results show at least two independent sets of oncornavirus-related sequences in the DNA from some leukemia patients. While the mode, source, and time of acquisition, and relevance to etiology, must be speculative, interspecies transmission of one or both agents may be involved.MATERIALS AND METHODS Purification of DNA from Human Tissues. The tissues used in these experiments were uncultured specimens obtained at the time of autopsy and kept frozen at -80°until the isolation of DNA. The organ tissues obtained from people with neoplasia were infiltrated with tumor cells. The tissues were lysed in a