Isolation of Isosativan from Nigerian Red Propolis

Abstract

Propolis has been known for its medicinal use and as a source of diverse bioactive natural compounds. Phytochemical screening of propolis samples usually reveal the constituent compounds and the source of the sample. Chromatography is the simplest way of isolating natural compounds and thus the hexane and ethyl acetate extracts of propolis from Bonny in Rivers State, Nigeria were subjected to column chromatography and the isolated compounds were identified by LC-MS and NMR analysis. The compounds detected and identified were oleic acid, propyl stearate, β-amyrin, isosativan, calycosin, liquiritigenin, isoliquiritigenin, pinocembrin and medicarpin. The structure of Isosativan was further confirmed by HRMS and 2D NMR spectroscopy. This is the first report of Isosativan from a sample of Nigerian and African propolis.

Physicochemical Assessment and Drug Potential of Some Phenylpropanoid and Flavonoid Compounds of Ethyl Acetate Eluate from Umudike Propolis

“…The dried ethyl acetate extracts were subjected to column chromatography using ethyl acetate throughout over silica gel (230-400 mesh ASTM). 24…”

The Strong Anti-Kinetoplastid Properties of Bee Propolis: Composition and Identification of the Active Agents and Their Biochemical Targets

“…Substitution of ring B to position C-3 instead of C2 produces the isoflavones (F5), isoflavanones (F6), and other moieties such as isoflavans (Structure F7) and pterocarpans (Structure F8). Many flavonoids have been identified in propolis; recent examples include isosativan, (2 -hydroxy-7,4 -dimethoxyisoflavan), liquiritigenin (45), isoliquiritigenin, formononetin (46), vestitol (21), neovestitol, medicarpin, 7-O-neovestitol, pinobanksin (47), pinocembrin, chrysin, and pinobanksin-3-O-acetate, astrapterocarpan, 3,8-dihydroxy-9-methoxy-pterocarpan, broussonin B, 8-prenylnaringenin (20), and gerontoxanthone H (12) [37][38][39].…”

“…There is currently an intense search for alternative safe anti-Leishmania chemotherapy from propolis. Several studies have shown that propolis obtained from diverse origins possesses antileishmanial activity due to the presence of flavonoids [16][17][18][19][20][21][22][23][24] (37)) was found to possess much better antileishmanial activity than Ecuadorian propolis samples rich in triterpenic alcohols and acetyl triterpenes and inhibited the growth of L. amazonensis amastigotes and promastigotes [63]; structures are shown in Figure 6. One of the common factors usually evaluated when investigating the pharmacological activities of propolis is isolating the active component and testing it against a pathogen.…”