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Propolis has been known for its medicinal use and as a source of diverse bioactive natural compounds. Phytochemical screening of propolis samples usually reveal the constituent compounds and the source of the sample. Chromatography is the simplest way of isolating natural compounds and thus the hexane and ethyl acetate extracts of propolis from Bonny in Rivers State, Nigeria were subjected to column chromatography and the isolated compounds were identified by LC-MS and NMR analysis. The compounds detected and identified were oleic acid, propyl stearate, β-amyrin, isosativan, calycosin, liquiritigenin, isoliquiritigenin, pinocembrin and medicarpin. The structure of Isosativan was further confirmed by HRMS and 2D NMR spectroscopy. This is the first report of Isosativan from a sample of Nigerian and African propolis.

An isolate of Newcastle disease virus (NDV) was obtained from a feral dove, (Stigmatopelia senegalensis). The isolate was shown to have a mean-death-time of 96 h and an intracerebral pathogenicity index of 0.10. It was immunogenic but not pathogenic for 6-week old chicks on experimental infection. Based on these observations the isolate was classified as a lentogenic strain. The role of such isolates of NDV from wild birds on the Nigerian poultry industry is discussed.