Isolation of Lactic Acid Bacteria with Cholesterol-Lowering Activity from Digestive Tracts of Indonesian Native Chickens

Julendra, Hardi; Suryani, Ade Erma; Istiqomah, L.; Damayanti, Ema; Anwar, Muslih; Fitriani, Naditya

doi:10.5398/medpet.2017.40.1.35

Cited by 16 publications

(11 citation statements)

References 17 publications

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“…In the previous in vitro studies, we reported that both probiotics performed cholesterollowering activities (Julendra et al, 2017;Istiqomah et al, 2018). The cholesterol-lowering activities of L. plantarum AKK-30 and S. cerevisiae B-18 were 17.4% and 51.8% respectively (Table 2).…”

Section: Viability and Potency In Cholesterol-loweringmentioning

confidence: 87%

“…The binding of bile acids by β-glucans in the human intestine were able to eliminate in bile acid pool and improved cholesterol breakdown. In previous studies (Julendra et al, 2017;Istiqomah et al, 2018) found that viability and cholesterol lowering activity of S. cerevisiae B-18 was higher than L. plantarum AKK-30. Yalçın et al (2010) reported that supplementation of S. cerevisiae in diet reduced cholesterol content in blood and egg yolk of laying hens.…”

Section: Blood Biochemical Profilesmentioning

confidence: 94%

“…Therefore it is essential to minimize the cholesterol level in eggs, for instance, through the administration of microbial additives. In our previous in vitro studies, L. plantarum (Julendra et al, 2017) or S. cerevisiae (Istiqomah et al, 2018) as probiotics had been shown to possess cholesterol-lowering activity. The positive impact of probiotics on the performance of the laying quail was previously reported by several studies (Hosseini, 2011;Guclu, 2011;Manafi et al, 2016;Siadati et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Cholesterol-lowering activity of lactic acid bacteria and yeast when used as probiotics in laying quail (Coturnix coturnix Japonica)

Istiqomah

Sakti

Sofyan

et al. 2020

J. Indonesian Trop. Anim. Agric.

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This study aimed to evaluate the administration of Lactobacillus plantarum AKK-30 and Saccharomyces cerevisiae B-18 as probiotics in reducing cholesterol of blood, egg, and meat and also the production performance of laying quail (Coturnix coturnix Japonica). A number of 600 birds of the twenty one-day-old quails were distributed in a completely randomized design with 5 treatments and 6 replications (initial BW= 101.35 ± 1.64 g). Treatments were as follows: A = negative control (basal diet without probiotic), B = 1% of L. plantarum AKK-30 (107 cfu/g), C = 1% of S. cerevisiae B-18 (106 cfu/g), D = 1% of a probiotic consortium, E = positive control (commercial probiotic). Parameters observed were blood biochemistry profiles, product quality, and production performance. The results revealed that administration of 1% S. cerevisiae (C) resulted the lowest cholesterol in blood (101.75 mg/dL) and egg (9.44 mg/g) and while administration of 1% L. plantarum(B) increased meat protein and decreased fat content (P<0.05). Level of blood triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were not different among treatments. Probiotic treatments did not affect the growth performance, quail day production (QDP), feed conversion ratio (FCR) and egg uniformity. However, probiotic increased diameter of egg yolk (P<0.05). It was concluded that administration of single probiotic L. plantarum AKK-30 or S. cerevisiae B-18 improves the quality of laying quail products.

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Section: Viability and Potency In Cholesterol-loweringmentioning

confidence: 87%

Section: Blood Biochemical Profilesmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Cholesterol-lowering activity of lactic acid bacteria and yeast when used as probiotics in laying quail (Coturnix coturnix Japonica)

Istiqomah

Sakti

Sofyan

et al. 2020

J. Indonesian Trop. Anim. Agric.

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“…The Ca-lactic acid is seen as clear zones around the colony on the medium [19]. The present of CaCO3 in the growing medium is an early step of LAB detection, where LAB show clear zones after the incubation period [20].…”

Section: A Isolation Of Lab From Indigenous West Sumatera Fermented mentioning

confidence: 99%

Isolation and Characterization of Lactic Acid Bacteria Producing GABA from Indigenous West Sumatera Fermented Food

Anggraini¹,

Marlida²,

Mirzah³

et al. 2019

Int. J. Adv. Sci. Eng. Inf. Technol.

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The purpose of this study was to isolate and characterize lactic acid bacteria (LAB) from indigenous West Sumatera fermented foods. The aim was to obtain LAB isolates that have the ability to produce γ-aminobutyric acid (GABA) and to characterize the highest GABA producing LAB. The indigenous West Sumatera fermented foods sampled were dadih (fermented buffalo milk), asam durian (fermented durian), tape singkong (fermented cassava) and ikan budu (fermented fish). The conventional method was used to isolate LAB, after which LAB were screened for γ-amino butyric acid (GABA) and GABA producing LAB characterized by biochemical tests. A total of 704 isolates were successfully isolated. Five hundred fifty two (552) isolates were identified as LAB and 103 isolates were confirmed as GABA producing LAB. The highest GABA producing LAB was DS15 and produced 49.365 mg/ml of GABA. DS18 produced the least GABA of 3.415 mg/ml. Biochemical assay of DS15 showed that the isolate could grow on the MRSA, undergoes aerobic respiration, do not use lactose, glucose, mannitol, arginine, aesculin, arabinose, raffinose, sorbitol, trehalase and xylose/melezitose. The isolate DS15 was also negative for arginine, nitrate reduction, catalase and oxidase tests. Based on the gram stain reaction, the isolate DS15 was a gram-positive bacterium and could be classified as Lactobacillus sp.

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“…Over the last decade, the investigation on the identification, characterization, and utilization of microbes isolated from GIT of chicken increased rapidly. Some of them reported the use of lactic acid bacteria as a probiotic agent for chicken (Musikasang et al, 2009;Damayanti et al, 2014;Jannah et al, 2017;Hamida et al, 2015;Yeh et al, 2017;Julendra et al, 2017). Meanwhile, the information about the lactic acid bacteria isolated from the GIT of Indonesian ducks for poultry probiotic agent is still limited.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Cellulolytic Lactic-Acid Bacteria from Mentok (Anas moschata) Gastro-Intestinal Tract

Herdian

Istiqomah

Damayanti

et al. 2018

Trop. Anim. Sci. J.

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Mentok, a local Indonesian name's of muscovy duck (Anas moschata), has been known as a duck with the capability to consume poor quality feed, high with non-starch carbohydrate (NSP) content. This capacity occurs because of the presence of microbial fermentation activity in the gastro intestinal tract (GIT) of mentok. However, the information about the identification and characterization of the cellulolytic microbes involved is limited. This study was expected to provide scientific contributions about gastrointestinal microbes, especially Lactic Acid Bacteria (LAB), with cellulolytic activities. The experiment was conducted to select LAB with cellulolytic activity from the GIT of mentok. Twenty six of selected LABs were isolated from the duodenum, cecum, and colon regions after microbiological characterization, i.e., morphology, catalase test, gas production, Gram staining, and motility test. Characterization for cellulolytic activity was analyzed by measuring the clearing zone on Carboxymethylcellulose (CMC) media, cell growth analysis on 1% CMC as a carbon source, and CMCase value. Pediococcus acidilactici MK 20 isolate from colon region was selected LAB with the highest cellulolytic activity with the clearing zone diameter, and the CMCase value of 2.33 mm and 0.0153 U/mL, respectively. Molecular identification using 16S rRNA gene sequences analysis revealed that P. acidilactici MK 20 isolate has 99% similarity with P. acidilactici strain ZW001. It was concluded that P. acidilactici MK 20 isolated from the colon part of the gastrointestinal tract of mentok, the Indonesian muscovy duck (A. moschata) had cellulolytic activity.

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Isolation of Lactic Acid Bacteria with Cholesterol-Lowering Activity from Digestive Tracts of Indonesian Native Chickens

Cited by 16 publications

References 17 publications

Cholesterol-lowering activity of lactic acid bacteria and yeast when used as probiotics in laying quail (Coturnix coturnix Japonica)

Cholesterol-lowering activity of lactic acid bacteria and yeast when used as probiotics in laying quail (Coturnix coturnix Japonica)

Isolation and Characterization of Lactic Acid Bacteria Producing GABA from Indigenous West Sumatera Fermented Food

Isolation of Cellulolytic Lactic-Acid Bacteria from Mentok (Anas moschata) Gastro-Intestinal Tract

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