Isolation of lindane- and endosulfan-degrading bacteria and dominance analysis in the microbial communities by culture-dependent and independent methods

Wu, Siang Chen; Gao, Jian-Kai; Chang, Bo-Sheng

doi:10.1016/j.micres.2021.126817

Cited by 14 publications

(3 citation statements)

References 62 publications

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“…also dominant in this experimentation, has been described in literature as harboring a Lin B gene encoding for HCH dehalogenation 47 , and even though not yet directly involved in HCH dehalogenation, the combination of our data and the one of the other authors suggest the involvement of the genus in HCH depletion. Despite not being directly characterized as HCH transforming bacterial species, candidates belonging to the genera Brevundimonas , Cellulomonas and Hyphomicrobium were found to be part of microbial consortia capable to deplete HCH 48–50 . Similarly, a Stenotrophomonas sp.…”

Section: Discussionmentioning

confidence: 99%

Co-Metabolic Growth and Microbial Diversity: Keys for the depletion of the α, δ, β and γ-HCH isomers

Bernabei,

De Simone,

Becarelli

et al. 2024

Preprint

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The objective of this study was to select and enhance microbiomes capable of degrading the α, δ, β and γ-HCH isomers. These microbiomes were isolated and enriched from an HCH-contaminated dumpsite in Italy, both in the presence of HCH isomers (1:1:1:1) as the sole carbon sources and under co-metabolic growth conditions in presence of glucose (0.1%). Four microbiomes were assessed for their relevant metabolic capabilities. A quantitative metabarcoding approach was employed to analyze the compositional evolution of the four microbiomes during the enrichment phase and the phase of tsting of the HCH isomers degradation kinetics. The use of a co-metabolic substrate during enrichment process was essential for selecting microbiomes with higher biodiversity. All microbiomes efficiently degraded the α, δ, and γ-HCH isomers. The highest efficiency in the β-HCH degradation capacity was associated to the highest biodiversity of the microbiome, and the involvement ofChryseobacteriumandAsinibacteriumsps. has been proposed for a recorded increment in bacterial load during the HCH degradation process.Statement of environmental implicationsSoil contaminated with hexachlorocyclohexane (HCH), including all four isomers, poses a significant risk to environmental and public health. This study isolates and selects microbiomes capable of degrading HCH, demonstrating their degradation efficiency using GC-MS analysis, and studies the microbial communities through metabarcoding of both the initial soils and the selected microbiomes. The contaminated soil originates from the historically polluted area of Italy known as SIN-Valle del Sacco. Developing and optimizing microbiome selection techniques for application on contaminated sites can significantly enhance soil bioremediation, thereby reducing contamination and protecting the environment.

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Section: Discussionmentioning

confidence: 99%

Co-Metabolic Growth and Microbial Diversity: Keys for the depletion of the α, δ, β and γ-HCH isomers

Bernabei,

De Simone,

Becarelli

et al. 2024

Preprint

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“…Enrichment culture is an effective method for the isolation of difficult-to-culture microorganisms from environmental samples. Wu et al isolated several bacteria with the ability to degrade environmental pollutants (including lindane and endosulfan) from their enrichment culture [ 30 ]. Moreover, Pernicova et al added activated sludge to the enrichment medium (MSM), which was rapidly detected by ATR-FTIR, and Bacillus with polyhydroxyalkanoate (PHA)-producing ability was also isolated [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Multiple Rounds of Enrichment on Metabolite Accumulation and Microbiota Composition of Pit Mud for Baijiu Fermentation

Liu

Zong

et al. 2023

Foods

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Pit mud (PM) is the main component of Baijiu (traditional Chinese liquor), and its microorganisms are the primary sources of the aroma of Chinese strong-flavor Baijiu (SFB). Enrichment plays an important role in the selection of functional microorganisms in PM. Herein, the PM of SFB was submitted to six rounds of enrichment using clostridial growth medium (CGM), and changes in the metabolite accumulation and microbiota composition were evaluated. Based on the metabolite production and microbiota composition, the enrichment rounds were classified as the acclimation stage (round 2), main fermentation stage (rounds 3 and 4), and late fermentation stage (rounds 5 and 6). Species within the genus Clostridium dominated in the acclimation stage (65.84–74.51%). In the main fermentation stage, the dominant microbial groups were producers of butyric acid, acetic acid, and caproic acid, which included Clostridium (45.99–74.80%), Caproicibacter (1.45–17.02%), and potential new species within the order of Oscillataceae (14.26–29.10%). In the late stage of enrichment, Pediococcus dominated (45.96–79.44%). Thus, the main fermentation stage can be considered optimal for the isolation of acid-producing bacteria from PM. The findings discussed herein support the development and application of functional bacteria by bioaugmentation, and contribute to improving the quality of PM and SFB production.

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“…Both physical and chemical treatment have their drawbacks, and biological treatment is seen as a promising solution. Hydrocarbon polluted soil and oil spills in the ocean can also be treated with this method [1][2][3][4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Activation energy, Temperature Coefficient and Q10 Value Estimations of the Growth of Alcaligenes sp. YLA11 on Diesel

Yakasai

Abubakar

2022

Bull Environ Sci Sust Manage

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Several models can be used to mimic the temperature-dependent growth rate of microorganisms on different media. Arrhenius is a popular model because of its small number of parameters. Microbial growth and metabolic activity on their substrates are generally affected by temperature. Microbes are vulnerable to temperature changes because of their small size. With a chevron-like discontinuous graph of apparent activation energy and a breakpoint, growth on diesel by Alcaligenes sp. YLA11 is described showing a breakpoint at 28.05 °C. Regression analysis resulted in two activation energies: 20–27 °C and 30–42 °C with the activation energies of 41.72 kJ/mol and 84.72 kJ/mol, respectively. For the examined temperature range (30-42 °C), a Q10 value of 2.905 and a theta value of 1.11 was calculated. Predicting the breakdown of diesel and its movement during bioremediation is an output that can be predicted by parts of this study.

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Isolation of lindane- and endosulfan-degrading bacteria and dominance analysis in the microbial communities by culture-dependent and independent methods

Cited by 14 publications

References 62 publications

Co-Metabolic Growth and Microbial Diversity: Keys for the depletion of the α, δ, β and γ-HCH isomers

Co-Metabolic Growth and Microbial Diversity: Keys for the depletion of the α, δ, β and γ-HCH isomers

Effect of Multiple Rounds of Enrichment on Metabolite Accumulation and Microbiota Composition of Pit Mud for Baijiu Fermentation

Activation energy, Temperature Coefficient and Q10 Value Estimations of the Growth of Alcaligenes sp. YLA11 on Diesel

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