Isolation of &lt;i&gt;Ascaris suum&lt;/i&gt; Components Which Suppress IgE Antibody Responses

Soares, Maria Fernanda Macedo; Mota, I.; Macedo, Mahasti Sahihi de

doi:10.1159/000236093

Cited by 33 publications

(38 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indeed, the presence of similar high molecular weight components in E-Asc was confirmed when this extract was fractionated on a Sephacryl S300 chromatography column and the suppressive effect of the first peak was demonstrated on the IgE antibody response to OA (21). Using the same experimental protocol, additional suppressive components were also identified in the third peak which contains low molecular mass proteins (29 kDa), in contrast to the results obtained with PIII isolated from the adult body extract (16,17). The presence of egg-specific antigens, not found in adult worms, had been already reported in A. suum by Justus and Ivey (25).…”

Section: Discussionmentioning

confidence: 67%

“…The extract was previously fractionated by gel filtration and three peaks, named PI, PII and PIII, were obtained. PI components were responsible for the suppression of the OA-specific response, whereas PIII components were not suppressive and stimulated anti-adult worm extract IgE antibodies (16,17). In vivo treatment with anti-IL-4 and anti-IL-10 monoclonal antibodies in OA plus adult worm extractimmunized mice abolishes the effects of adult A. suum body extract on the Th1-related parameters of the immune response to OA.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extracts of Ascaris suum egg and adult worm share similar immunosuppressive properties

Souza

Faquim-Mauro

Macedo

2002

Braz J Med Biol Res

View full text Add to dashboard Cite

Adult Ascaris suum body extract (Asc) prepared from male and female worms (with stored eggs) down-regulates the specific immune response of DBA/2 mice to ovalbumin (OA) and preferentially stimulates a Th2 response to its own components, which is responsible for the suppression of the OA-specific Th1 response. Here, we investigated the participation of soluble extracts prepared from male or female worms or from eggs (E-Asc) in these immunological events. Extracts from either sex (1 mg/animal) or E-Asc (0.35 or 1 mg protein/ animal) suppressed the delayed-type hypersensitivity (DTH) reaction (60-85%), proliferative response (50-70%), IL-2 and IFN-g secretion (below detection threshold) and IgG1 antibody production (70-90%) of DBA/2 mice to OA. A dose of 0.1 mg E-Asc/animal did not change DTH or proliferation, but was as effective as 0.35 mg in suppressing IL-2 and IFN-g, and OA-specific IgG1 antibodies. Lymph node cells from DBA/2 mice injected with Asc (1 mg/animal) or a high dose of E-Asc (1 mg protein/animal) secreted IL-4 upon in vitro stimulation with concanavalin A. As previously demonstrated for Asc, the cytokine profile obtained with the E-Asc was dose dependent and changed towards Th1 when a low dose (0.1 mg protein/animal) was used. Taken together, these results suggest that adult worms of either sex and eggs induce the same type of T cell response and share similar immunosuppressive properties.

show abstract

Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Extracts of Ascaris suum egg and adult worm share similar immunosuppressive properties

Souza

Faquim-Mauro

Macedo

2002

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…This observation is unusual in that, in most cases, both B and T cells are subject to the effects of inhibitory factors from helminths. For example, a phosphorylcholine-containing preparation of Brugia malayi (16) and an extract of A. suum (13,39,40) have been demonstrated to inhibit the functional activities of both B and T cells. Interestingly, an earlier report by Price and Turner (33) the cells 24 h prior to the addition of mitogens.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in contrast to the immunostimulatory activity of the body fluid of Ascaris reported above, Ferreira and coworkers (13) and Soares and coworkers (39,40) have reported immunomodulatory factors in secretions of A. suum, which ablate B-cell responses. Similarly, the nematode Heligmosomoides polygyrus has been reported to contain immunomodulatory factors capable of either stimulating (37,38) or inhibiting (33,34) T-and B-cell responses.…”

mentioning

confidence: 86%

Modulation of B-Cell Proliferative Response by a Soluble Extract ofNippostrongylus brasiliensis

2000

View full text Add to dashboard Cite

We and others have previously shown that nematodes or nematode products can stimulate or inhibit the generation of lymphocyte responses, suggesting that nematodes exert diverse effects on the developing immune responses of their host. In this study we examined the immunomodulatory effect of a soluble extract of Nippostrongylus brasiliensis (adult worm homogenate [AWH]) on B-cell responsiveness. We found that the extract inhibited the proliferation of B cells to lipopolysaccharide (LPS) stimulation in a dose-dependent manner. This effect was specific to B cells, since the extract did not inhibit T-cell proliferation to concanavalin A or anti-CD3 stimulation. The data presented here confirm that the extract is not toxic to B cells. We present evidence that the active factor is proteinaceous in nature and that the inhibitory activity is restricted to the adult stage of Nb. The extract does not appear to interfere with early activation events since it can be added up to 48 h after LPS stimulation, and it inhibited responses to phorbol myristate acetate and ionomycin. Furthermore, the proliferation of B cells to other activators was also inhibited by AWH. This observation shows that the inhibitory activity of AWH is not restricted to LPS-mediated B-cell proliferation. We present evidence that, in the absence of accessory cells, the inhibitory effect of the extract was ablated. This observation shows that the activity of AWH is not mediated directly on B cells but is mediated via the production of negative signals from accessory cells (macrophages), which affect a downstream pathway required by all B-cell activators tested. These effects on B-cell and accessory cell function are likely to have a significant effect on the outcome of infections experienced concurrently.To ensure survival and continuation of their life cycle, nematodes have evolved a unique relationship with their hosts. Part of this accommodation is the ability to modulate host immune responsiveness (25). Modulation of lymphocyte function has been widely reported among nematodes (2,3,19,21,22,28) and other parasites (6,15,27,36). For example, we have previously shown a dramatic potentiation of antibody responses to third party antigens by treatment with a body fluid extract of Ascaris suum (19). This observation is significant in that it suggests that the modulatory effect of nematodes on immune responses is far-reaching and may have profound effects on developing immune responses to unrelated antigenic challenge. We have also reported the presence of a B cell mitogen in the body fluid of A. suum, which stimulated G 0 B cells to enter the cell cycle (20). This activity of the Ascaris factor was dependent on viable accessory cells.The effect of helminth products on the developing immune response is likely to be complex. For example, in contrast to the immunostimulatory activity of the body fluid of Ascaris reported above, Ferreira and coworkers (13) and Soares and coworkers (39, 40) have reported immunomodulatory factors in secretions of A. suum, which a...

show abstract

“…The isolation of protein fractions from A. suum extract by Sephacryl S-300 gel filtration chromatography yields three peaks (PI, PII, and PIII). PI is constituted by high weight components that suppress anti-OVA IgE antibody production in mice immunized with OVA + PI (Soares et al, 1992;Faquim-Mauro & Macedo, 1998). Thus, the suppressive effect observed in A. suum extract is due to high molecular weight components.…”

Section: Pas-1 An Anti-inflammatory Protein From Ascaris Suummentioning

confidence: 99%

Derived Products of Helminth in the Treatment of Inflammation, Allergic Reactions and Anaphylaxis

Araújo¹,

Macedo-Soares²

2012

Allergic Diseases - Highlights in the Clinic, Mechanisms and Treatment

View full text Add to dashboard Cite

Isolation of <i>Ascaris suum</i> Components Which Suppress IgE Antibody Responses

Cited by 33 publications

References 15 publications

Extracts of Ascaris suum egg and adult worm share similar immunosuppressive properties

Extracts of Ascaris suum egg and adult worm share similar immunosuppressive properties

Modulation of B-Cell Proliferative Response by a Soluble Extract ofNippostrongylus brasiliensis

Derived Products of Helminth in the Treatment of Inflammation, Allergic Reactions and Anaphylaxis

Contact Info

Product

Resources

About