1978
DOI: 10.1111/j.1432-1033.1978.tb12053.x
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Isolation of Messenger RNA Coding for Eggshell Protein of the DNA‐Eliminating Nematode Ascaris lumbricoides

Abstract: Poly(A)‐containing RNA from polyploid uterine epithelial cells of Ascaris lumbricoides has been isolated by poly(U)‐Sepharose chromatography. The bulk of poly(A)‐containing RNA migrates as 18‐S RNA in formamide/polyacrylamide gels. In a cell‐free wheat germ system, this RNA directs the synthesis of a polypeptide with identical migration behavior in dodecylsulphate/urea/polyacrylamide gels as the polypeptide isolated from the proteinaceous eggshell. The two proteins reveal almost identical peptide patterns in f… Show more

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