Isolation of Methyl Gallate from Toxicodendron sylvestre and Its Effect on Tomato Bacterial Wilt

Abstract

Yuan, G.-Q., Li, Q.-Q., Qin, I., Ye, Y.-E, and Lin, W. 2012. Isolation of methyl gállate from Toxicodendron sylvestre and its effect on tomato bacterial wilt. Plant Dis. 96:1143-1147.We determined that the methanol extract of Toxicodendron sylvestre could significantly inhibit Ralstonia solanacearum in vitro and in planta. One compound responsible for the antibacterial activity was isolated from the ethyl ether extract and identified as methyl gállate (MG) based on its 'H and '^C nuclear magnetic resonance data as well as mass spectroscopy. MG displayed broad-spectrum activity against plant-pathogenic bacteria, and strong inhibitory effects on the growth of plant-pathogenic oomycetes. The half inhibition concentration of MG on R. solanacearum was 8.3 mg/liter, which was tested by the agar dilution method. Disease control trials in planta showed that both natural and synthetic MG could effectively reduce the incidence of tomato bacterial wilt, and there was no significant difference between them in control efñcacy. This is the first report on the use of MG for the control of a plant bacterial disease. Because of its broad-spectrum antimicrobial activity and significant control efficacy on tomato bacterial wilt in planta, MG shows potential to be used as a bactéricide to control plant bacterial wilt.

Methyl Gallate From Jiringa (Archidendron Jiringa) and Antioxidant Activity.

“…Data NMR of proton H isolated methyl gallate from T. sylvestre was shown peak at δ7.1 and δ3.4. This peak identification protons H at position 2 and 6, H bounded with OCH 3 [6]. This data also agreement with Cheng et al had been isolated methyl gallate from Chinese toon.…”

“…Data NMR of carbon methyl gallate had isolated from seed coats T. sylvestre was peaks at δ167.3 identificated carbon at position -COOH, δ146.1 (position carbon at 3 and 5), δ138.8 (position carbon at 4), δ121.9 (position carbon at 1), δ109.9 (position carbon at 2 and 6), and δ52.0 (-OCH 3 ). Data NMR of carbon methyl gallate had isolated from seed coats Chinese toon was peaks at δ167.8 identificated carbon at position -COOH, δ145.3 (position carbon at 3 and 5), δ138.6 (position carbon at 4), δ120.2 (position carbon at 1), δ108.8 (position carbon at 2 and 6), and δ51.1 (-OCH 3 ) [3,4,6,24].…”

“…The radical scavenging effect of methyl gallate has high activity [4][5][6]. Methyl gallate is derivative of gallic acid.…”

Fungicidal Activity of Essential Oils from Cinnamomum cassia against the Pathogenic Fungi of Panax notoginseng Diseases

“…Naturally occurring antimicrobial compounds have received much attention in recent times. Previous studies show that many plant‐derived compounds, including methyl gallate and protocatechualdehyde, suppress the survival of the soilborne pathogen Ralstonia pseudosolanacearum with strong antibacterial effects . Consequently plant organic compounds have potential for effective biological control of plant diseases.…”

Plant-derived Antibacterial Metabolites Suppressing Tomato Bacterial Wilt Caused by Ralstonia solanacearum

“…Methyl gallate was isolated from the methanol extract of Toxicodendron sylvestre. It significantly inhibited RSSC in vitro and in planta (Yuan et al, 2012). It exhibited antibacterial activity against RSSC, with MIC and minimum bacteriocidal concentration values of 20 and 30 µg/ml, respectively.…”