Isolation of molybdenum cofactor defective cell lines of Nicotiana tabacum

Buchanan, Roger J.; Wray, John L.

doi:10.1007/bf00332680

Cited by 29 publications

(9 citation statements)

References 30 publications

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“…The same is true of all putative Mpt-mutants in numerous other organisms (8,9,11,14,43,53,58,61,68), with the sole exception of the Neurospora crassa nit-i mutant (33). We have applied both a refined nit-i reconstitution assay procedure and a direct physicochemical assay for MPT to the analysis of potential Mpt-mutants in E. coli, and we report definitive characterization of true Mpt-mutants in E. coli.…”

mentioning

confidence: 83%

Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis

Johnson¹,

Rajagopalan²

1987

J Bacteriol

102

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All molybdenum enzymes except nitrogenase contain a common molybdenum cofactor, whose organic moiety is a novel pterin called molybdopterin (MPT). To assist in elucidating the biosynthetic pathway of MPT, two MPT-deficient mutants of Escherichia coli K-12 were isolated. They lacked activities of the molybdenum enzymes nitrate reductase and formate dehydrogenase, did not reconstitute apo nitrate reductase from a Neurospora crassa nit-1 strain, and did not yield form A, a derivative of MPT. By P1 mapping, these two mutations mapped to chlA and chlE, loci previously postulated but never definitely shown to be involved in MPT biosynthesis. The two new mutations are in different genetic complementation groups from previously isolated chlA and chlE mutations and have been designated as chlM and chlN (closely linked to chlA and chlE, respectively). The reported presence of Mo cofactor activity in the chlA1 strain is shown to be due to in vitro synthesis of MPT through complementation between a trypsin-sensitive macromolecule from the chlA1 strain and a low-molecular-weight compound from the nit-l strain.

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mentioning

confidence: 83%

Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis

Johnson¹,

Rajagopalan²

1987

J Bacteriol

102

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“…This design was first used for Arabidopsis (Oostindier-Braaksma & Feenstra, 1973) and further, was successfully used for higher plants (Buchanan & Wray, 1982). The toxic effect of chlorate is caused by the reduction of the relatively innocuous chlorate to poisonous chlorite (and/or its decomposition products), which is also catalyzed by NR.…”

Section: Selection Of Lethal Clo − 3 Concentrationmentioning

confidence: 99%

Isolation and characterization of a nitrate reductase deficient mutant of Chlorella ellipsoidea (Chlorophyta)

et al. 2005

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Using X-ray mutagenesis and a chlorate-resistance selection method, a nitrate reductase (NR)-deficient mutant of Chlorella ellipsoidea nrm-4 was isolated, which is incapable of using NO − 3 as a nitrogen source. NR activity was not detected in nrm-4, suggesting this is a null mutation. Molecular analysis revealed that nrm-4 has a two base deletion at position 2348-49 of its DNA sequence, which produced a frame-shift mutation. The nrm-4 mutation is stable and nrm-4 algae could only use NH + 4 and NO − 2 as nitrogen sources. Expression of a wild-type NR gene could complement this NR-deficient mutant. Furthermore, this transgenic strain was able to grow in NO − 3 medium, when the growth rate of the nrm-4 strain was equal to that of the wide type alga. The nrm-4 strain could potentially be used as a bioreactor that uses nitrate as a selectable marker instead of an antibiotic or herbicide.Abbreviations: NR: nitrate reductase.

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“…The initial chl nomenclature was later changed to moa-mog for genes involved in molybdenum cofactor biosynthesis and nar for nitrate reductase genes [12, 19–22]. Subsequent to these germinal studies, similar pleiotropic mutant strains were also reported in Pseudomonas aeruginosa [23], Klebsiella aerogenes [24], Drosophila melanogaster [25, 26] and Nicotiana tabacum [27]. Additional studies by Nason and coworkers [28–31] identified a specific pleiotropic Neurospora crassa mutant termed nit-1 , which was incapable of growth on nitrate or xanthine.…”

Section: The Discovery Of Mocomentioning

confidence: 99%

The history of the discovery of the molybdenum cofactor and novel aspects of its biosynthesis in bacteria

Leimkühler

Wuebbens

Rajagopalan

2011

Coordination Chemistry Reviews

123

154

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Biosynthesis of the molybdenum cofactor in bacteria is described with a detailed analysis of each individual reaction leading to the formation of stable intermediates during the synthesis of molybdopterin from GTP. As a starting point, the discovery of molybdopterin and the elucidation of its structure through the study of stable degradation products are described. Subsequent to molybdopterin synthesis, the molybdenum atom is added to the molybdopterin dithiolene group to form the molybdenum cofactor. This cofactor is either inserted directly into specific molybdoenzymes or is further modified by the addition of nucleotides to the molybdopterin phosphate group or the replacement of ligands at the molybdenum center.

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Isolation of molybdenum cofactor defective cell lines of Nicotiana tabacum

Cited by 29 publications

References 30 publications

Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis

Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis

Isolation and characterization of a nitrate reductase deficient mutant of Chlorella ellipsoidea (Chlorophyta)

The history of the discovery of the molybdenum cofactor and novel aspects of its biosynthesis in bacteria

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