Isolation of NDM-1-producing multidrug-resistant Pseudomonas putida from a paediatric case of acute gastroenteritis, India

Bhattacharya, Debdutta; Dey, Shuchismita; Kalal, Shanta; Jali, Sujata; Koley, Hemanta; Sinha, Ritam; Nag, Dhrubajyoti; Kholkute, S. D.; Roy, Subarna

doi:10.1016/j.nmni.2015.02.002

Cited by 14 publications

(11 citation statements)

References 17 publications

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“…It causes a variety of different infections, from easy-to-cure ear infections, serious infections of burn patients, to severe lung infections which lead to major complications in cystic fibrosis patients (Barbier and Wolff, 2010 ; Azzopardi et al, 2014 ). Besides Pseudomonas aeruginosa , other species e.g., Pseudomonas putida or Pseudomonas fluorescens are also a cause for infections in clinical settings (Gilarranz et al, 2013 ; Erol et al, 2014 ; Bhattacharya et al, 2015 ; Mazurier et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Patterns of Pseudomonas spp. Isolated from the River Danube

et al. 2016

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Spread and persistence of antibiotic resistance pose a severe threat to human health, yet there is still lack of knowledge about reservoirs of antibiotic resistant bacteria in the environment. We took the opportunity of the Joint Danube Survey 3 (JDS3), the world's biggest river research expedition of its kind in 2013, to analyse samples originating from different sampling points along the whole length of the river. Due to its high clinical relevance, we concentrated on the characterization of Pseudomonas spp. and evaluated the resistance profiles of Pseudomonas spp. which were isolated from eight sampling points. In total, 520 Pseudomonas isolates were found, 344 (66.0%) isolates were identified as Pseudomonas putida, and 141 (27.1%) as Pseudomonas fluorescens, all other Pseudomonas species were represented by less than five isolates, among those two P. aeruginosa isolates. Thirty seven percent (37%) of all isolated Pseudomonas species showed resistance to at least one out of 10 tested antibiotics. The most common resistance was against meropenem (30.4%/158 isolates) piperacillin/tazobactam (10.6%/55 isolates) and ceftazidime (4.2%/22 isolates). 16 isolates (3.1%/16 isolates) were multi-resistant. For each tested antibiotic at least one resistant isolate could be detected. Sampling points from the upper stretch of the River Danube showed more resistant isolates than downriver. Our results suggest that antibiotic resistance can be acquired by and persists even in Pseudomonas species that are normally not in direct contact with humans. A possible scenario is that these bacteria provide a reservoir of antibiotic resistance genes that can spread to related human pathogens by horizontal gene transfer.

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Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Patterns of Pseudomonas spp. Isolated from the River Danube

et al. 2016

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“…Because hospital isolates of P . putida have been studied in terms of their resistance to antibiotics (i.e., [ 9 , 21 , 28 – 31 ]), and more recently in terms of their potential to colonize and cause human tissue damage [ 32 ] we chose to focus on identifying genes present within P . putida clinical isolates that mediate nosocomial infections and survival in hospital settings.…”

Section: Introductionmentioning

confidence: 99%

Specific Gene Loci of Clinical Pseudomonas putida Isolates

et al. 2016

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Pseudomonas putida are ubiquitous inhabitants of soils and clinical isolates of this species have been seldom described. Clinical isolates show significant variability in their ability to cause damage to hosts because some of them are able to modulate the host’s immune response. In the current study, comparisons between the genomes of different clinical and environmental strains of P. putida were done to identify genetic clusters shared by clinical isolates that are not present in environmental isolates. We show that in clinical strains specific genes are mostly present on transposons, and that this set of genes exhibit high identity with genes found in pathogens and opportunistic pathogens. The set of genes prevalent in P. putida clinical isolates, and absent in environmental isolates, are related with survival under oxidative stress conditions, resistance against biocides, amino acid metabolism and toxin/antitoxin (TA) systems. This set of functions have influence in colonization and survival within human tissues, since they avoid host immune response or enhance stress resistance. An in depth bioinformatic analysis was also carried out to identify genetic clusters that are exclusive to each of the clinical isolates and that correlate with phenotypical differences between them, a secretion system type III-like was found in one of these clinical strains, a determinant of pathogenicity in Gram-negative bacteria.

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“…Occurrences of NDM have been reported from many countries including Asian countries such as China, Bangladesh, India, Vietnam [3,[8][9][10][11][12][13][14][15][16]. Among many variants, NDM-5 has been reported to possess increased resistance to carbapenems and broad-spectrum cephalosporins and was first reported in 2011 in an Escherichia coli isolate from United Kingdom [10].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Computational Modelling of New Delhi Metallo-β-Lactamase-5 from an Escherichia coli Isolate (KOEC3) of Bovine Origin

et al. 2016

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Emergence of antimicrobial resistance mediated through New Delhi metallo-β-lactamases (NDMs) is a serious therapeutic challenge. Till date, 16 different NDMs have been described. In this study, we report the molecular and structural characteristics of NDM-5 isolated from an Escherichia coli isolate (KOEC3) of bovine origin. Using PCR amplification, cloning and sequencing of full blaNDM gene, we identified the NDM type as NDM-5. Cloning of full gene in E. coli DH5α and subsequent assessment of antibiotic susceptibility of the transformed cells indicated possible role of native promoter in expression blaNDM-5. Translated amino acid sequence had two substitutions (Val88Leu and Met154Leu) compared to NDM-1. Theoretically deduced isoelectric pH of NDM-5 was 5.88 and instability index was 36.99, indicating a stable protein. From the amino acids sequence, a 3D model of the protein was computed. Analysis of the protein structure elucidated zinc coordination and also revealed a large binding cleft and flexible nature of the protein, which might be the reason for broad substrate range. Docking experiments revealed plausible binding poses for five carbapenem drugs in the vicinity of metal ions. In conclusion, results provided possible explanation for wide range of antibiotics catalyzed by NDM-5 and likely interaction modes with five carbapenem drugs.

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Isolation of NDM-1-producing multidrug-resistant Pseudomonas putida from a paediatric case of acute gastroenteritis, India

Cited by 14 publications

References 17 publications

Antibiotic Resistance Patterns of Pseudomonas spp. Isolated from the River Danube

Antibiotic Resistance Patterns of Pseudomonas spp. Isolated from the River Danube

Specific Gene Loci of Clinical Pseudomonas putida Isolates

Molecular Characterization and Computational Modelling of New Delhi Metallo-β-Lactamase-5 from an Escherichia coli Isolate (KOEC3) of Bovine Origin

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