Isolation of novel benzo[a]anthracene-degrading microorganisms and continuous bioremediation in an expanded-bed bioreactor

Rosales, Emílio; Paz, Alicia; Vázquez, Xosé H.; Pazos, Marta

doi:10.1007/s00449-011-0669-x

Cited by 24 publications

(13 citation statements)

References 15 publications

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“…Total BAA removal was obtained after 7 days. This fact is a promising result because less is known about the bacteria capable of utilizing PAHs containing four or more rings as a carbon and energy source, and it has been reported that only fungi can degrade these types of PAHs (Peng et al 2008;Rosales et al 2012).…”

Section: Resultsmentioning

confidence: 99%

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Assessment of Arthrobacter viscosus as reactive medium for forming permeable reactive biobarrier applied to PAHs remediation

Ferreira

Cobas

Tavares

et al. 2013

Environ Sci Pollut Res

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Polycyclic aromatic hydrocarbons (PAHs) are significant environmental contaminants as they are present naturally as well as anthropogenically in soil, air and water. In spite of their low solubility, PAHs are spread to the environment, and they are present in surface water, industrial effluent or groundwater. Amongst all remediation technologies for treating groundwater contaminated with PAHs, the use of a permeable reactive biobarrier (PRBB) appears to be the most cost-effective, energy efficient, and environmentally sound approach. In this technology, the microorganisms are used as reactive medium to degrade or stabilize the contaminants. The main limits of this approach are that the microorganisms or consortium used for forming the PRBB should show adequate characteristics. They must be retained in the barrier-forming biofilm, and they should also have degradative ability for the target pollutants. The aim of the present work is to evaluate the viability of Arthrobacter viscosus as bioreactive medium for forming PRBB. Initially, the ability of A. viscosus to remove PAHs, benzo[a]anthracene 100 μM and phenanthrene 100 μM was evaluated operating in a batch bench-scale bioreactor. In both cases, total benzo[a]anthracene and phenanthrene removals were obtained after 7 and 3 days, respectively. Furthermore, the viability of the microorganisms was evaluated in the presence of chromium in a continuous mode. As a final point, the adhesion of A. viscosus to sepiolite forming a bioreactive material to build PRBB was demonstrated. In view of the attained results, it can be concluded that A. viscosus could be a suitable microorganism to form a bioreactive medium for PAHs remediation.

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Section: Resultsmentioning

confidence: 99%

“…The polluted medium (PM) was composed of malt extract 3 g/L, yeast extract 3 g/L, Tween 80 1 %, acetone 2 % and 100 μM PAHs (Moscoso et al 2012a, b;Rosales et al 2012). In the experiment where the presence of metals was evaluated, this medium also contained 100 mg/L of Cr(VI).…”

Section: Polluted Mediamentioning

confidence: 99%

Assessment of Arthrobacter viscosus as reactive medium for forming permeable reactive biobarrier applied to PAHs remediation

Ferreira

Cobas

Tavares

et al. 2013

Environ Sci Pollut Res

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“…(1) was used to model the obtained data, in which D is the PAHs degradation degree at a specific moment of the time t, D 0 and D max are the initial and maximum degradation percentage, respectively, and l D is the maximum specific degradation rate (Rosales et al 2012).…”

Section: Growth and Biodegradation Assays In Flasksmentioning

confidence: 99%

Biodegradation of PAHs by Acinetobacter isolated from karst groundwater in a coal-mining area

Shao

Wang

et al. 2015

Environ Earth Sci

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A bacterial strain Acinetobacter sp. WSD with phenanthrene-degrading ability was identified based on biochemical tests and 16S rDNA gene sequence analysis. The strain was isolated from polycyclic aromatic hydrocarbons (PAHs)-contaminated groundwater from a coalmining area of the Guozhuang karst water system in Shanxi province of northern China. Acinetobacter sp. WSD could utilize fluorine (FLO), phenanthrene (PHE) and pyrene (PYR) as its sole carbon source and was able to degrade other PAHs. Approximately 90 % of FLO, 90 % of PHE and 50 % of PYR were degraded after 6 days' incubation. The logistic model well fitted all the experimental data. The specific degradation rates in glucose were higher than that in HCO 3 -, indicating that organic carbon source promoted the growth of Acinetobacter sp. WSD and the degradation of PAHs. In presence of humic acids (HA), the total degradation rate was accelerated, although there was a delay at the beginning. In the case of high HA concentration, co-metabolism between PAHs and HA occurred. The metabolic pathway for the three compounds of PAHs was discussed using GC-MS data. Phthalic acid was found in their metabolites. Phenol, 2,5-bis(1,1-dimethylethyl), a new type of PAHs metabolites that have been reported before was found after 2 days degradation.

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“…Stability of PAHs in the soil is also dependent on the structure and properties of hydrocarbons [Banach-Szott et al 2012]. Usually, low-molecular-weight PAHs are easily degradable, but highmolecular weight PAHs, containing four or more aromatic rings, are considered resistant, thermodynamically stable and more difficult to degrade [Rosales et al 2012].…”

Section: Introductionmentioning

confidence: 99%

CHANGES THE CONCENTRATION OF SELECTED PAHs IN URBAN SOILS FERTILIZED WITH MUNICIPAL SEWAGE SLUDGE

et al. 2015

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The aim of this study was to analyze changes in the content of selected polycyclic aromatic hydrocarbons (PAHs) in urban soils fertilized with municipal sewage sludge. The factors in the experiment were three lawns along the main roads in Bialystok (in Popieluszki, Hetmanska and Raginisa Strs.), doses of sewage sludge (0 -control; 75 and 150 Mg/ha) and two years of study (2011 and 2012). The studied parameters were monitored at the end of the growing season (in October 2011 and 2012) by determining fluoranthene, pyrene, benzo[a]anthracene, chrysene, and benzo[a]pyrene in soil samples. Furthermore, the dehydrogenase activity, the pH and organic carbon content of the soil were determined. Obtained content of studied PAHs in the urban soil does not exceed those contained in the Regulation of the Minister of the Environment of September 9 th , 2002 on soil quality standards and ground quality standards. The concentration of pyrene and chrysene differed significantly depending on the time of sampling and the localization. It has been shown that fertilization of soil sewage sludge revealed reduction of pyrene concentration in soil. Among 5 tested PAHs compounds, benzo[a]pyrene was the most dominant compound both in samples collected in 2011 and 2012 (28 and 27% respectively).

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Isolation of novel benzo[a]anthracene-degrading microorganisms and continuous bioremediation in an expanded-bed bioreactor

Cited by 24 publications

References 15 publications

Assessment of Arthrobacter viscosus as reactive medium for forming permeable reactive biobarrier applied to PAHs remediation

Assessment of Arthrobacter viscosus as reactive medium for forming permeable reactive biobarrier applied to PAHs remediation

Biodegradation of PAHs by Acinetobacter isolated from karst groundwater in a coal-mining area

CHANGES THE CONCENTRATION OF SELECTED PAHs IN URBAN SOILS FERTILIZED WITH MUNICIPAL SEWAGE SLUDGE

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