Isolation of Pectin from coffee pulp Arabica Gayo for the development of matrices membrane

Abstract

Pectin from Arabica Gayo coffee pulp has been successfully isolated using citric acid as a solvent. The pectin extracted from the coffee pulp is determined by the extraction conditions. This study aims to produce pectin from dried coffee extract using citric acid using two factors, namely the difference factor of citric acid (1: 5 and 1: 20 b/v) and extraction time 75 and 150 minutes. The optimum ratio of Arabica Gayo coffee pulp with citric acid was 1:20 b/v with rendemen 7,8 % with 125 minute extraction time and at the temperature of 80 °C and pH 4. The methoxyl content of isolated pectin was 12.71 % and has been determined as high methoxyl pectin (HMP). The result of analysis with FTIR is known that the extract produced is pectin with comparison with pectin standard. The pectin has been used as a matrix membrane and showed its smooth surface gel form and smaller diameter from scanning electron microscopy (SEM).1

Construction of a Hydrogel Pectin-Based Triglyceride Optical Biosensor with Immobilized Lipase Enzymes

“…The construction of the TG optical biosensor consisted of the preparation of a pH optical sensor [26,32] that was modified with the addition of a lipase enzyme. Lipase will catalyze the transformation of a TG into a fatty acid, which will be detected by the pH optical sensor.…”

“…However, membranes from synthetic materials possess inefficient permeability, and in the long run are detrimental to the environment. Hence, biopolymeric materials like pectin [32] will be an excellent choice for a biosensor matrix. Biopolymers are superior in terms of biocompatibility, capability of enzyme absorption, thermal stability, and not to mention environmentally friendliness.…”

“…Biopolymers are superior in terms of biocompatibility, capability of enzyme absorption, thermal stability, and not to mention environmentally friendliness. Pectin, for instance, has been recognized for its hydrogel properties, which can facilitate the interaction of the active compounds and analytes within the matrix [32,33].…”

Coffee Pulp: An Industrial By-product with Uses in Agriculture, Nutrition and Biotechnology

“…In the pharmaceutical industry, pectin extracted from coffee pulp (Hasanah et al, 2019) could be used as biodegradable membrane matrices for controlled drug release. Pectin and other coffee pulp components are also being investigated as hepatoprotective agents (Ontawong et al, 2019) to lessen the harmful effects of exogenous or endogenous toxins in this high metabolic activity organ.…”

“…Coffee pulp represents approximately 40 to 50% weight of the (Rolz et al, 1980;Hernandez et al, 2009; and its composition fluctuates depending on the coffee varieties; for example, wide ranges of macronutrients have been reported (per 100 g of dry matter): 4-12 g of protein, 1-2 g of lipids, and 45-89 g of carbohydrates (Oliveira and Franca, 2014;Salinas-Rios et al, 2014b), including pectin (Hasanah et al, 2019). Minerals such as P, K, Ca and Mg have been reported at 2.48, 25.13, 4.10 and 1.39 g/kg of coffee pulp, respectively, and those for Fe and Mn, at 77 and 46 mg/kg (Fierro-Cabrales et al, 2018).…”