Isolation of phenotypically distinct trophoblast cell lines from normal rat chorioallantoic placentas

Hunt, Joan S.; Deb, Sanjukta; Faria, Teresa N.; Wheaton, David; Soares, Michael J.

doi:10.1016/0143-4004(89)90038-6

Cited by 28 publications

(21 citation statements)

References 18 publications

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“…Established placental HRP-1 [18] and RCHO-1 [19,20] cells were maintained in RPMI 1640 medium, supplemented with 20% fetal bovine serum (heat inactivated), 100 lg/ml penicillin-streptomycin, 1 mM sodium pyruvate, and 50 lM 2-mercaptoethanol. TGFB1 and TGFB2 were purchased from Biosource (Invitrogen), and TGFB3 was from Calbiochem (San Diego, CA).…”

Section: Cell Lines and Reagentsmentioning

confidence: 99%

“…Rat and mouse models have provided valuable insights into the molecular mechanisms that occur during trophoblast differentiation and invasion; however, these models do not necessarily translate to the human, because of different reproductive physiology [17]. The present study offers the rationale for investigating the effect of each TGFB isoform to activate signaling pathways and to modulate the proliferation and invasion of two rat placental cell lines: HRP-1 cells, which were derived from a normal midgestation chorioallantoic placenta [18], and RCHO-1 cells, which were established from a choriocarcinoma [19,20]. The results from the present study suggest the involvement and the importance of TGFB in the invasion of rat placental cell lines.…”

Section: Introductionmentioning

confidence: 99%

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Transforming Growth Factor Beta Regulates Proliferation and Invasion of Rat Placental Cell Lines1

Lafontaine¹,

Chaudhry²,

Lafleur³

et al. 2011

Biology of Reproduction

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Implantation of an embryo in the endometrium is a critical step for continuation of pregnancy, and implantation failure is a major cause of infertility. In rats, the implantation process involves invasion of the endometrial epithelial lining by the trophoblastic cells in order to reach the underlying stromal cells. Transforming growth factor beta (TGFB) is a multifunctional cytokine that regulates proliferation, differentiation, and invasiveness of multiple cell lineages. We used rat HRP-1 and RCHO-1 placental cell lines to perform this study. HRP-1 cells were derived from midgestation chorioallantoic placental explants of the outbred Holtzman rat, whereas RCHO-1 cells were established from a rat choriocarcinoma. MTT proliferation assays revealed that each TGFB isoform decreased HRP-1 cell growth in a dose-dependent manner, whereas RCHO-1 cells were resistant to the growth-suppressive effect of TGFB1 and TGFB3. Only TGFB2 reduced RCHO-1 cell proliferation. Activation of ERK, MAPK14 (p38 MAPK), or SMAD pathways is known to play a role in cell proliferation, and we found that TGFB activates these pathways in both HRP-1 and RCHO-1 cells in an isoform-specific manner. MTT proliferation assays revealed that ERK pathway is partially implicated in TGFB3-reduced HRP-1 cell proliferation. Hoechst nuclear staining and caspase-3 cleavage demonstrated that TGFB isoforms failed to induce apoptosis in both cell lines. Matrigel invasion assays showed that both HRP-1 and RCHO-1 cells exhibit intrinsic invasive ability under untreated conditions. The capacity of HRP-1 cells to invade the Matrigel was selectively increased by TGFB2 and TGFB3, whereas all TGFB isoforms could increase the invasiveness of RCHO-1 cells. These important functional studies progressively reveal a key role for TGFB in regulating proliferation and invasiveness of placental cells.

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Section: Cell Lines and Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transforming Growth Factor Beta Regulates Proliferation and Invasion of Rat Placental Cell Lines1

Lafontaine¹,

Chaudhry²,

Lafleur³

et al. 2011

Biology of Reproduction

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show abstract

“…The rat trophoblast cell lines Rcho-1 (Faria and Soares, 1991) R8RP.3, HRP-1 and LRP-2 (Hunt et al, 1989) and the mouse trophoblast cell line SM-9 (J.S. Hunt, unpublished) were maintained in RPMI-1640 supplemented with Lglutamine, 1 mM sodium pyruvate, 50 µM β-ME and 10% FBS.…”

Section: Cell Culturementioning

confidence: 99%

Repression of MHC class II gene transcription in trophoblast cells by novel single-stranded DNA binding proteins

et al. 1997

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“…The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to, and retains characteristic expression of, cellular markers of labyrinthine trophoblast cells, in which the bulk of maternal-fetal nutrient/waste transfer occurs (13,14,20). This cell line has proved to be a useful in vitro model system to study function and regulation of several transporters, including glutamate transporters (22), fatty acid transporters (17), and a facilitative glucose transporter (5).…”

mentioning

confidence: 99%

Characterization of an organic anion transport system in a placental cell line

Zhou¹,

Tanaka²,

Soares³

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

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Transporters within the placenta play a crucial role in the distribution of nutrients and xenobiotics across the maternal-fetal interface. An organic anion transport system was identified on the apical membrane of the rat placenta cell line HRP-1, a model for the placenta barrier. The apical uptake of 3H-labeled organic anion estrone sulfate in HRP-1 cells was saturable ( Km = 4.67 μM), temperature and Na+ dependent, Li+ tolerant, and pH sensitive. The substrate specificity of the transport system includes various steroid sulfates, such as β-estradiol 3,17-disulfate, 17β-estradiol 3-sulfate, and dehydroepiandrosterone 3-sulfate (DHEAS) but does not include taurocholate, p-aminohippuric acid (PAH), and tetraethylammonium. Preincubation of HRP-1 cells with 8-bromo-cAMP (a cAMP analog) and forskolin (an adenylyl cyclase activator) acutely stimulated the apical transport activity. This stimulation was further enhanced in the presence of IBMX (a phosphodiesterase inhibitor). Together these data show that the apical membrane of HRP-1 cells expresses an organic anion transport system that is regulated by cellular cAMP levels. This transport system appears to be different from the known taurocholate-transporting organic anion-transporting polypeptides and PAH-transporting organic anion transporters, both of which also mediate the transport of estrone sulfate and DHEAS.

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Isolation of phenotypically distinct trophoblast cell lines from normal rat chorioallantoic placentas

Cited by 28 publications

References 18 publications

Transforming Growth Factor Beta Regulates Proliferation and Invasion of Rat Placental Cell Lines1

Transforming Growth Factor Beta Regulates Proliferation and Invasion of Rat Placental Cell Lines1

Repression of MHC class II gene transcription in trophoblast cells by novel single-stranded DNA binding proteins

Characterization of an organic anion transport system in a placental cell line

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