Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig

Davoli, Roberta; Zambonelli, Paolo; Bigi, Daniele; Gellin, Joël; Yerle, Martine M.; Milc, Justyna Anna; Braglia, Silvia; Cenci, Valentina; Cagnazzo, M.; Russo, Vincenzo

doi:10.1046/j.1365-2052.2002.00800.x

Cited by 83 publications

(45 citation statements)

References 41 publications

(65 reference statements)

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“…The fragments were cloned and if the PCR product did not exactly match the expected length were sequenced to verify the product (see also additional information, Table 1). Furthermore, 309 sequenced clones of an adult-pig-muscle-specific cDNA library (Davoli et al, 2001) including many muscle structural and energy metabolism-related genes were placed on the microarray. In total the microarray contained 557 genes with known identity and function.…”

Section: Microarray Constructionmentioning

confidence: 99%

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Pas

Wit

Priem

et al. 2005

J Muscle Res Cell Motil

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Myogenesis, the formation of muscle fibers, is a complex process. Pigs have been selected for efficient muscle growth for the past decades making them interesting to study myogenesis. We studied expression profiles of genes known to affect myogenesis, muscle structural proteins, and energy metabolism in prenatal pigs from 14 to 91 days of gestation. Primary and secondary muscle fiber formation takes place during days 30-60 and 54-90 of gestation, respectively. Differential expression and expression levels of the genes were studied using microarray technology. Gene activation and repression profiles were studied counting the number of spots with detectable signal. The number of spots for muscle tissue structural protein genes showing upregulated expression increased constantly from day 14 until day 91of gestation indicating continued activation of genes during this period. The mRNA expression level of the genes showed a peak around day 35 of gestation. The expression levels of genes affecting myogenic differentiation (stimulating and inhibiting) showed a peak at day 35 of gestation. The number of spots for differentiation-stimulating genes showing differential expression reaches a first peak around day 35 of gestation and a nadir at day 49 of gestation while the number of spots for differentiation-inhibiting genes reaches a nadir at day 35 of gestation. Myogenic differentiation seems less a matter of the expression level of genes affecting differentiation, but depends on the balance between the number of significantly activated genes for stimulating and inhibiting differentiation. Genes stimulating myoblast proliferation showed a small peak expression prior to day 35 of gestation indicating myoblast proliferation before differentiation. The number of spots and the expression levels of genes for glycolysis and ATP-metabolism are at a nadir around days 35 and 49-63 of gestation suggesting that the energy metabolism is low during fusion of myoblasts into multinucleated muscle fibers.

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Section: Microarray Constructionmentioning

confidence: 99%

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Pas

Wit

Priem

et al. 2005

J Muscle Res Cell Motil

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“…Studies have shown that the porcine TTID gene is associated with the SSC2q24-q29 QTL marker (Davoli et al, 2002). The SSC2 region is considered to have some QTLs for average backfat, average daily gain, meat androstenone content, 16-day weight, drip loss, off-flavor, pH, and also other traits (Thomsen et al, 2004;Liu et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that TTID plays an indispensable role in stabilizing and anchoring of thin filaments, which may be a prerequisite for correct Z-disc organization (Salmikangas et al, 2003). In porcine, Davoli et al (2002) only mapped the gene to SSC 2q26.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of the porcine TTID gene and association of different TTID genotypes with carcass traits

et al. 2014

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ABSTRACT. The titin immunoglobulin domain (TTID) protein localizes to the Z line in muscle and binds to alpha-actinin and gamma-filamin. It plays an indispensable role in stabilizing and anchoring of thin filaments. In this study, the 5'-regulatory region of the porcine TTID gene was analyzed with bioinformatic methods. Another objective of this study was to further investigate the polymorphism in the intron 6 of the porcine TTID gene. We determined allele frequency among six Chinese porcine purebreds. The polymorphisms were genotyped in a population of 280 F 2 pigs representing two Large White x Meishan reference families. Different TTID genotypes were significantly associated with carcass traits, including skin percentage (P < 0.05), loin eye area (P < 0.05), and average skin thickness (P < 0.01). Our study will continue to lay the groundwork for further investigations into the detailed function of the porcine TTID gene.

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“…Currently, eight kinds of MyHC isoforms, which are encoded by separate genes, have been found in mammals, and are located on chromosomes 7 and 12 (Weiss et al, 1999;Shrager et al, 2000;Davoli et al, 2002). Only MyHCI, MyHCIIA, and MyHCIIX are expressed in sheep skeletal muscle.…”

Section: Spatial and Temporal Expression Pattern Of Yap1 And Myhcsmentioning

confidence: 99%

Sheep YAP1 temporal and spatial expression trend and its relation with MyHCs expression

Gao

Sun

et al. 2016

Genet. Mol. Res.

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ABSTRACT. RT-PCR was used to study the temporal and spatial pattern of Yes-associated protein 1 (YAP1) and myosin heavy chain (MyHC) expression in four different skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and three growth stages (i.e., 2 days old, 2 and 6 months old) of Hu Sheep. The results showed that YAP1 was differentially expressed in skeletal muscles of sheep, that expression increased gradually with age, and that there were high levels of expression in the gastrocnemius muscle and lower levels in the longissimus dorsi muscle. MyHCI was expressed at high levels in the soleus muscle and at lower levels in the longissimus dorsi muscle. In contrast, MyHCIIA and MyHCIIX were expressed at high levels in the extensor digitorum longus and at lower levels in the soleus muscle. The expression of MyHCI and MyHCIIA decreased with increasing age while that of MyHCIIX increased. YAP1 expression was negatively correlated with MyHCII (P < 0.01) and positively correlated with MyHCIIX (P < 0.01) across all growth stages and skeletal muscle types studied. We speculate that after birth, the thicker muscle fiber diameter is associated with the high expression of MyHCIIX. Therefore, we conclude that YAP1 expression affects sheep muscle fiber development after birth and provides important genetic information for the selection candidate genes for sheep muscle growth.

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Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig

Cited by 83 publications

References 41 publications

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Transcriptional analysis of the porcine TTID gene and association of different TTID genotypes with carcass traits

Sheep YAP1 temporal and spatial expression trend and its relation with MyHCs expression

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