Isolation of porcine follicular fluid inhibin of 32K daltons

Miyamoto, Kaoru; Hasegawa, Yoshihisa; Fukuda, Masaki; Nomura, Mitsuo; Igarashi, Masao; Kangawa, Kenji; Matsuo, Hisayuki

doi:10.1016/0006-291x(85)90164-0

Cited by 343 publications

(117 citation statements)

References 12 publications

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“…Inhibin is a dimeric protein originally isolated from porcine and bovine follicular fluid as an FSH-suppressing protein (Ling et al 1985, Miyamoto et al 1985, Rivier et al 1985, Robertson et al 1985. Thereafter, inhibin proved to be a major FSH-suppressing factor during the rat oestrous cycle (Rivier et al 1986, Arai et al 1996.…”

Section: Figurementioning

confidence: 99%

Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle

Fafioffe

Jf²,

Fontaine³

et al. 2004

Journal of Endocrinology

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In mammals, activin and inhibin are important regulators of FSH secretion. Previous studies have demonstrated that primary ovine pituitary cells express different activin receptor subtypes: activin receptor-like (ALK)2, ALK4, activin type II receptor A (ActRIIA), ActRIIB and Smad proteins in vitro. Here, we have carried out physiological studies to investigate the pattern of mRNA expression of the activin receptor subunits in the ewe pituitary throughout the oestrous cycle. The oestrous cycles of ewes were synchronized with progestagen sponges. The animals were killed 36 h (before the preovulatory surge, n=4), 48 h (during the preovulatory surge, n=4), 72 h (during the second surge of FSH, n=6) and 192 h (during the luteal phase, n=4) after sponge removal. Using Northern blots, we have shown that the levels of ALK2, ALK4 and ActRIIB mRNA were significantly higher before the preovulatory surge and during the secondary surge of FSH as compared with both during the preovulatory surge and the luteal phase, whereas the level of the ActRIIA mRNA was similar throughout the oestrous cycle. Using Western blots we have also demonstrated that the level of phosphoSmad2 did not vary during the reproductive cycle. Inhibin binding protein (InhBP/p120) and the transforming growth factor-type III receptor, betaglycan, have been identified as putative inhibin co-receptors. In this study, we cloned a fragment of both InhBP/p120 and betaglycan cDNAs in the ewe and showed by Northern blot that pituitary betaglycan and InhBP/p120 mRNA levels did not fluctuate across the oestrous cycle nor did they correlate with serum FSH levels.

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Section: Figurementioning

confidence: 99%

Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle

Fafioffe

Jf²,

Fontaine³

et al. 2004

Journal of Endocrinology

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“…Activins and inhibins were identified initially as gonadal proteins which have a stimulating (activins) and suppressing (inhibins) effect on follicle-stimulating hormone (FSH) production in cultured rat anterior pituitary cells (Ling et al 1985, Miyamoto et al 1985, Vale et al 1986. Activins are homodimers of the b subunits (bA and bB) and the different dimerization of subunits gives rise to three glycoproteins: activin A (bA-bA), activin B (bB-bB) and activin AB (bA-bB) (Mason et al 1985).…”

Section: Introductionmentioning

confidence: 99%

Production and localization of activins and activin type IIA and IIB receptors by the human endosalpinx

Refaat

Bahathiq²,

Sockanathan³

et al. 2004

Reproduction

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Fallopian tubes from ten premenopausal women were collected and examined for the presence of inhibin, activin and its type IIA and IIB receptors (ActRIIA and ActRIIB) in the endosalpinx. Immunocytochemistry demonstrated clear staining for the bA, bB subunits and ActRIIA and ActRIIB that increased in intensity from the isthmus to the ampulla. No staining for the a subunit was observed. Whilst the staining of the bA subunit and ActRIIA was seen in almost every epithelial cell, staining for the bB subunit and ActRIIB was more variable. In situ hybridization and RT-PCR confirmed the presence of mRNA for the bA, bB subunits and ActRIIA and ActRIIB. These results indicated that the epithelium of the uterine tube is able to synthesize activin but not inhibin and has receptors for activin. Activins may thus act as paracrine regulators of tubal epithelial cell function, and embryonic activity may also bind to epithelial receptor and initiate intracellular processes that alter epithelial cell secretions.

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“…Activins are either BMBA (activin A) and BB/13B (activin B) subunit homodimers or BA/BB (activin AB) subunit heterodimers, and inhibins are ~6A (inhibin A) and tyJBB (inhibin B) subunit heterodimers (2). Activins and inhibins were originally isolated on the basis of their capacity to stimulate and inhibit the release of follicle-stimulating hormone (FSH) from the pituitary, respectively (3)(4)(5)(6)(7)(8). Activins are also thought to be important regulators of early developmental processes in vertebrates; they are expressed during chick and Xenopus embryogenesis and can induce axial structures in these species (9,10).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of 5′‐regulatory region of mouse activin ßa subunit gene

et al. 1998

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SUMMARY:We isolated genomic clones that contain the 5'-flanking region of the mouse activin BA subunit gene. The nucleotide sequence determination of the 5'-flanking region of the gene and the comparison of that with the reported mouse cDNA structure identified the putative 5' regulatory region, a novel first exon and a part of the first intron of the gene within this region. The putative 5' regulatory region of the mouse activin BA subunit gene directed the expression of CAT gene in transfected HT 1080 cells. Successive deletions of this region demonstrated a 400-bp region that exerts a strong positive effect on promoter activity of the mouse activin fSA subunit gene.

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Isolation of porcine follicular fluid inhibin of 32K daltons

Cited by 343 publications

References 12 publications

Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle

Activin and inhibin receptor gene expression in the ewe pituitary throughout the oestrous cycle

Production and localization of activins and activin type IIA and IIB receptors by the human endosalpinx

Isolation and characterization of 5′‐regulatory region of mouse activin ßa subunit gene

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