Isolation of pregnancy-associated glycoproteins from placenta of the American bison (Bison bison) at first half of pregnancy

Kiewisz, Jolanta; Sousa, Noelita Melo de; Beckers, J. F.; Vervaecke, Hilde; Panasiewicz, Grzegorz; Szafrańska, Bożena

doi:10.1016/j.ygcen.2007.04.011

Cited by 27 publications

(14 citation statements)

References 51 publications

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“…In addition, PVDF possesses good physical properties over temperatures ranging from 5-0 to 140 • C. So, PVDF membranes have been widely used in a variety of industrial applications; for example, fuel cells, water purification, wastewater treatment, food industry and medical applications. [1][2][3] The different applications require different morphology and performance of the PVDF membranes. Up to now, the adjustment of the membrane morphology has been one of the focuses of the research on the preparation of the membranes.…”

Section: Introductionmentioning

confidence: 99%

Morphology and Formation Mechanism of Poly(Vinylidene Fluoride) Membranes Prepared with Immerse Precipitation: Effect of Dissolving Temperature

Wang

Zhang

et al. 2009

Journal of Macromolecular Science, Part B

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Poly(vinylidene fluoride) (PVDF) membranes were prepared by the immersion precipitation method. The effects of polymer dissolving temperature for the dopes on the morphology, crystallization and performance of prepared membranes were examined. Polymer dissolving temperature was varied from 50 to 120 • C. N,N-dimethylacetamide (DMAc) and de-ionized water were used as solvent and non-solvent, respectively. Based on the membrane morphology and the schematic phase diagram of the ternary system, the membrane formation mechanism was analyzed theoretically. The binodal liquidliquid demixing took place first for the nucleation and growth of droplets in the polymer poor phase; then consequently the spinodal liquid-liquid demixing occurred in the polymer rich phase. The demixings together resulted in the prepared membranes having a cross-section composed of interconnected globule-like particulates with bi-continuous structured surfaces. The dissolving temperature of the dopes had a remarkable effect on the morphology of the cross-section, even when the solution underwent a long time cooling before the demixing. The increase of the diameter of the particulates with the dissolving temperature was theoretically analyzed according to the conditions of the polymeric solution.

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Section: Introductionmentioning

confidence: 99%

Morphology and Formation Mechanism of Poly(Vinylidene Fluoride) Membranes Prepared with Immerse Precipitation: Effect of Dissolving Temperature

Wang

Zhang

et al. 2009

Journal of Macromolecular Science, Part B

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“…In the bPAG family, oligosaccharide heterogeneity is caused by diversified tetra-antennary glycans [54]. In addition, three purified PAG isoforms (72, 74 and 76 kDa) secreted by the placenta of the American bison have been sequenced [55]. However, in the European bison, among two major groups (43–45 and 67–69 kDa) of immuno-detected secretory PAG isoforms [52], eleven novel diversified pregnancy-stage dependent (45–129 day post coitum–dpc) EbPAGs (50–71 kDa) have been sequenced [56].…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Placentally Expressed Aspartic Proteinase in Humans

Majewska

Lipka

Panasiewicz

et al. 2017

IJMS

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This study presents pioneering data concerning the human pregnancy-associated glycoprotein-Like family, identified in the genome, of the term placental transcriptome and proteome. RNA-seq allowed the identification of 1364 bp hPAG-L/pep cDNA with at least 56.5% homology with other aspartic proteinases (APs). In silico analyses revealed 388 amino acids (aa) of full-length hPAG-L polypeptide precursor, with 15 aa-signal peptide, 47 aa-blocking peptide and 326 aa-mature protein, and two Asp residues (D), specific for a catalytic cleft of the APs (VVFDTGSSNLWV91-102 and AIVDTGTSLLTG274-285). Capillary sequencing identified 9330 bp of the hPAG-L gene (Gen Bank Acc. No. KX533473), composed of nine exons and eight introns. Heterologous Western blotting revealed the presence of one dominant 60 kDa isoform of the hPAG-L amongst cellular placental proteins. Detection with anti-pPAG-P and anti-Rec pPAG2 polyclonals allowed identification of the hPAG-L proteins located within regions of chorionic villi, especially within the syncytiotrophoblast of term singleton placentas. Our novel data extend the present knowledge about the human genome, as well as placental transcriptome and proteome during term pregnancy. Presumably, this may contribute to establishing a new diagnostic tool for examination of some disturbances during human pregnancy, as well as growing interest from both scientific and clinical perspectives.

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“…Details on immunoblotting and Western blot techniques were previously described by Kiewisz et al [25]. Briefly, proteins were transferred onto a nitrocellulose membrane (0.45 μm, Protran BA85; Schleicher and Schuell Biosciences, Dassel, Germany) after SDS-PAGE.…”

Section: Methodsmentioning

confidence: 99%

“…PAG molecules were successfully purified and characterized in bovine (boPAG) [9], ovine (ovPAG) [19]–[21], caprine (caPAG) [22], water buffalo (wbPAG) [23,24], American bison (AmbPAG) [25] and European bison (EbPAG) [26] species. Huang et al [27] were the first to report the partial characterization of PSPB molecules in moose ( Alces alces ) and elk ( Cervus canadensis ) placenta.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, since the early 2000s, it was reported that lectins such as the agglutinins from Vicia Villosa agarose (VVA) bind to the N-acetyl galactosamine (GalNAc) of asparagine-linked glycans from PAG [31]. Therefore, VVA affinity chromatography has been applied with success to purify PAG molecules [23]–[25]. This paper describes the isolation and characterization of fallow deer PAG (fdPAG) proteins from placental extracts by using VVA and anti-PAG-2 affinity chromatographies.…”

Section: Introductionmentioning

confidence: 99%

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Identification of pregnancy-associated glycoproteins and alpha-fetoprotein in fallow deer (Dama dama) placenta

et al. 2014

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Background: This paper describes the isolation and characterization of pregnancy-associated glycoproteins (PAG) from fetal cotyledonary tissue (FCT) and maternal caruncular tissue (MCT) collected from fallow deer (Dama dama) pregnant females. Proteins issued from FCT and MCT were submitted to affinity chromatographies by using Vicia villosa agarose (VVA) or anti-bovine PAG-2 (R#438) coupled to Sepharose 4B gel. Finally, they were characterized by SDS-PAGE and N-terminal microsequencing. Results: Four distinct fallow deer PAG (fdPAG) sequences were identified and submitted to Swiss-Prot database.Comparison of fdPAG with PAG sequences identified in other ruminant species exhibited 64 to 83% identity. Additionally, alpha-fetoprotein was identified in fetal and maternal tissues. Conclusion: Our results demonstrate the efficacy of VVA and bovine PAG-2 affinity chromatographies for the isolation of PAG molecules expressed in deer placenta. This is the first report giving four specific amino acid sequences of PAG isolated from feto-maternal junction (FCT and MCT) in the Cervidae family.

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Isolation of pregnancy-associated glycoproteins from placenta of the American bison (Bison bison) at first half of pregnancy

Cited by 27 publications

References 51 publications

Morphology and Formation Mechanism of Poly(Vinylidene Fluoride) Membranes Prepared with Immerse Precipitation: Effect of Dissolving Temperature

Morphology and Formation Mechanism of Poly(Vinylidene Fluoride) Membranes Prepared with Immerse Precipitation: Effect of Dissolving Temperature

Identification of Novel Placentally Expressed Aspartic Proteinase in Humans

Identification of pregnancy-associated glycoproteins and alpha-fetoprotein in fallow deer (Dama dama) placenta

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