Isolation of primary osteoblast cell lines from adult rat and rat embryos and their use as models for in vitro biocompatibility tests of nanostructured titanium-based implants

Abstract

Methods for obtaining osteoblast cultures from the calvaria of adult Wistar rats and 12-day-old embryos of these rats have been adapted for studying the biocompatibility and ossointegration of titanium-based implants. The osteoblast morphology and their differentiation into osteocytes on a titanium matrix with specially treated surface have been studied. It has been confirmed that two cultures of diploid rat cells obtained in the study can serve as efficient models for preclinical in vitro testing of nanostructured titanium implants for biocompatibility and osseointegration.

Análise Do Percentual De Colágeno Na Dentina Humana Entre Diferentes Etnias, Gêneros E Idades

“…In the biocompatibility assays, NO acts as an immunostimulator of the inflammatory response, what means that the material in contact with the cell culture is cytotoxic, since the NO upon release tries to neutralize the material and recruit cells of the immune system, such as macrophages (Khrunyk et al, 2017). Silva (2008) related the synthesis of NO in its study with calcium hydroxide associated with chlorhexidine, stating that it does not induce the formation of NO, therefore it is not immunostimulatory.…”

“…However, they present shorter life time, resisting to at most 10 cellular passages (Hayflick, 1965). Different cell lines are usually obtained from animal sources (KHRUNYK Et Al, 2017;WANG et al, 2017) or from humans (Galeotti et al, 2013).…”