Isolation Of Pseudomonas Aeruginosa from Soil and Production of Lipase Enzyme

Abstract

The isolates of Pseudomonas aeruginosa bacteria were uncovered in the soil surrounding the roots of palms and public gardens in Baghdad for the production of lipase enzyme. The lipase enzyme has many applications that are included in the textile and food industry, and the manufacture of detergents and medical preparations. Several tests such as temperature change, incubation period, change of lipid sources, nitrogen sources such as peptone and tryptone, and carbon sources such as glucose and lactose were carried out to choose suitable conditions for bacterial growth. The results indicated studying the conditions affecting production, it was noted that the best production was when using the culture medium to which 1% of corn oil was added, pH 7, at a temperature of 37 °C and an incubation period of 24 hours in vibrating incubator at 151 rpm, The soil surrounding the roots of the plant is a good reservoir for the presence of Pseudomonas aeruginosa bacteria

Optimization of Culture Conditions for Lipase Production by Pseudomonas aeruginosa ECS3

“…This indicates that Pseudomonas aeruginosa can produce lipase at 35 o C. It appears that the lipase activity slightly increased at 45 o C, possibly due to environmental factors (Figure 2). Similarly, lipase production by Pseudomonas sp., Penicillium fellutanum, Kocuria flava Y4, and Providencia stuartii was stimulated at 35 o C. [26][27][28][29] Lipase from Staphylococcus sp., Bacillus cereus, and Geobacillus stearothermophilus, on the other hand, were maximally obtained at 37 o C, 30 45 o C, 31 and 60 o C, 32 respectively. This shows that the physical properties of the cell membrane, energy metabolism, and translational synthesis of proteins are affected by temperature.…”

“…However, some studies revealed an incubation period of 48h for maximum lipase production by Pseudomonas aeruginosa and Geobacillus stearothermophilus, respectively. 32,26 The short period puts this enzyme in an advantageous position because it tends to increase the rate of turnover of this enzyme production. A relationship exists between the incubation period and enzyme production to a certain degree, where enzyme activity tends to reduce beyond the optimum incubation condition.…”

“…35,28 Contrarily, the lipase of Pseudomonas aeruginosa was not enhanced by any carbon source as compared with the control. 26 Since lipases are inducible enzymes, carbon sources often promote their gene expression. Carbon sources, which supply energy for growth and enzyme production, are catabolized by carbon catabolite regulation (CCR).…”

تثبيط تكوين الاغشية الحيوية في بكتيريا Agrobacterium tumefaciens بالمادة الطافية الخالية من خلايا Pseudomonas aeruginosa والمحللة بجهاز GC-MS

“…To isolate the bacteria, 5 g of soil samples were diluted in 50 ml of phosphate-buffered saline solution and shaken for one hour. One ml of the sample was then grown in 100 mL of nutrient broth for 24 hours at 30°C and then grown on nutrient agar for 48 hours at 30°C 27 . The greenish-blue bacteria were selected and underwent microscopically examination, biochemical characterization (API 20E) kit, and 16S rRNA gene sequencing 28 , using the primer shown in Table 1, according to Edwards et al, 29 the Polymerase Chain Reaction (PCR) settings were 94°C for 5 minutes of initial denaturation, 35 cycles of 94°C for 1 minute, 58°C for 1 minute, 72°C for 1 minute, and 10 minutes of final extension at 72°C.…”