Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of &lt;i&gt;Azadirachta Indica&lt;/i&gt; and Antimicrobial and Cytotoxic screening of the Crude Extracts

Islam, Monirul; Al‐Amin, Abul Quasem; Siddiqi, M Mahboob Ali; Akter, Shakila; Haque, Mohammad Majedul; Sultana, Nazia; Chowdhury, AM Sarwaruddin

doi:10.3329/dujs.v60i1.10328

Cited by 24 publications

(19 citation statements)

References 9 publications

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“….00 (d) (C-5''), 61.14 (t) (C-6''). These data were found to be consistent with those reported in the literature for 3-O-β-D-glucopyranoside (Shi et al, 2016;Islam et al, 2012 andLi et al, 2008), and compound 3 identified as 3-O-β-D-glucopyranoside.…”

Section: Stigmast-4-en-3-one (2)supporting

confidence: 81%

“…Compound 3 obtained in the form of yellow powder with a melting point 872.6 °C and completely soluble in methanol, Fluorescent under ultraviolet light at a wavelength 254 and 360 nm, indicated there is founded conjugated double bonds. Comparison of physical data and physicochemical compound3.3-O-β-D-glucopyranoside or Isoquercetin shows a very high suitability (Shi et al, 2016;Islam et al, 2012 andLi et al, 2008), thus compound 3 was identified as 3-O-β-D-glucopyranoside or Isoquercetin.…”

Section: -O-β-d-glucopyranoside (3)mentioning

confidence: 99%

“…Crystal white needles; melting point 87-89ºC, 1 H NMR (Pyridin-D6, 600 MHz), -6.82 (1H, d, 8.4) 7.55 (1H, dd, 2.4; 8.4) 5.11 (1H, dd, 6.8; 7.2) 3.46 (1H, d, 9) 3.39 (1H, d, 9.6) 3.32 (1H, d, 9.6) 3.19 (1H, m) 3.68 (1H, dd, 2.4; 11.4) 3.56 (1H, dd, 6.0; 11.4) * 400 MHz for 1 H and 100 MHz for 13C, in CD3OD (Islam et al, 2012) and Measurements compound 3 were taken at 600 MHz for 1 H and 150 MHz for 13 C. -29). These data were found to be consistent with those reported in the literature for Stigmast-4-en-3-one (Halilu et al, 2013;Barla et al, 2006 andDellaGreca et al, 1990), and compound 2 identified as Stigmast-4-en-3-one.…”

Section: Stigmast-4-en-3-one (2)mentioning

confidence: 99%

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Steroids and isoquercetin from Lygodium microphyllum

2017

J App Pharm Sci

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Two steroids, stigma-5(6)-en-3β-ol or β-sitosterol (1) and Stigmast-4-en-3-one (2) and quercetin 3-O-β-Dglucopyranoside or Isoquercetin (3) has been isolated from an extract of n-hexane and ethyl acetate from the leaves of plants Lygodium microphyllum. The chemical structures of compounds 1, 2 and 3 identified based on spectroscopic data and comparison of spectral data obtained from the literature. The discovery of steroid compounds, stigma-5 (6) -en-3β-ol, Stigmast-4-en-3-one, and quercetin 3-O-β-D-glucopyranoside in plants L. microphyllum first reported in this study

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Section: Stigmast-4-en-3-one (2)supporting

confidence: 81%

Section: -O-β-d-glucopyranoside (3)mentioning

confidence: 99%

Section: Stigmast-4-en-3-one (2)mentioning

confidence: 99%

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Steroids and isoquercetin from Lygodium microphyllum

2017

J App Pharm Sci

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“…Dari 100 gram daun mimba kering diperoleh 18 gram ekstrak metanol. Ekstrak metanol daun mimba kemudian difraksinasi dan diperoleh fraksi etil asetat sebanyak 2 gram yang kemudian difraksinasi dan dimurnikan sehingga diperoleh isolat Q4G sebanyak 14,4 mg dan Q3G sebanyak 60,1 mg. [9][10][11][12] Dengan demikian, diketahui bahwa dari 1 kg daun mimba segar BPT Situbondo mengandung isolat Q3G sebesar 60,1 mg dan Q4G sebanyak 14,4 mg.…”

Section: Pembahasanunclassified

Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa

Rohmawaty¹,

Yunivita²

2016

mkb

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AbstrakDaun mimba (Azadirachta indica A.Juss) yang berasal dari BPT Situbondo memiliki zat aktif quercetin-3-Oglucoside (Q3G) dan quercetin-4-O-glucoside (Q4G) yang diketahui mempunyai kemampuan menghambat ambilan glukosa melalui membran usus halus. Penelitian ini bertujuan mengetahui potensi isolat dan dosis optimal Q3G dan Q4G dari daun mimba terhadap ambilan glukosa melalui membran usus halus tikus putih (Rattus rattus novergicus). Zat aktif Q3G dan Q4G dari daun mimba diisolasi secara kualitatif dengan metode Kromatografi Lapis Tipis (KLT). Penelitian ini dilakukan di beberapa tempat, antara lain: Laboratorium Kimia Organik FMIPA Unpad, Laboratorium Biokimia FK Unpad, serta Laboratorium Farmakologi dan Terapi FK Unpad. Penelitian dilakukan pada bulan Juni-Desember 2011. Penelitian laboratorium eksperimental dengan rancangan acak lengkap dilakukan terhadap 30 ekor tikus putih yang dibagi dalam 5 kelompok. Kelompok kontrol diberi larutan glukosa 3,0 x 10 -3 M, dan 4 kelompok perlakuan diberi larutan glukosa berturut-turut Q3G 1 mg/kgBB, Q3G 2 mg/kgBB, Q4G 1 mg/kgBB, dan Q4G 2 mg/kgBB menggunakan alat perfusi in situ. Kadar ambilan glukosa diamati setiap 15 menit selama 1 jam. Data dianalisis menggunakan one-way ANOVA dan tes Duncan dengan taraf kepercayaan 95% (α=0,05). Hasil penelitian menunjukkan sebanyak 53,1 mg isolat Q3G dan 14,4 mg Q4G diperoleh dari daun mimba BPT Situbondo sebanyak 1 kg. Isolat Q3G 1 mg/kgBB, Q4G 1 mg/kgBB, dan Q4G 2 mg/kgBB berbeda secara bermakna dalam menghambat ambilan glukosa melalui membran usus halus tikus dibanding dengan kontrol pada menit ke-45 (p<0,05). Simpulan, isolat Q3G dan Q4G dari daun mimba dapat menghambat ambilan glukosa melalui membran usus halus tikus putih. These compounds were isolated using the Thin Layer Chromatography (TLC). From previous studies, Q3G and Q4G are known to inhibit the glucose uptake from intestinal membrane. This study was conducted to understand the potential influence of Q3G and Q4G isolated from mimba leaves in inhibiting glucose uptake in rat's intestinal membrane (Rattus rattus norvegicus). This experimental study employed 30 male rats that met the inclusion criteria that were divided into 5 groups (n=6). Group I was the control group and only received glucose solution 3,0 x 10-3 M. Group II, III, IV, and V received glucose solution with Q3G 1 mg/kgBW, Q3G 2 mg/kgBW, Q4G 1 mg/ kgBW, and Q4G 1 2 mg/kgBW, respectively. The inhibitory potentials of Q3G and Q4G on glucose uptake was measured every 15 minutes for one hour using in situ perfusion equipment. Data were analyzed using One-way ANOVA and Duncan test with a significance level 95% (α=0.05). From 1 kg fresh mimba leaves, 60.1 mg Q3G and 14.4 mg Q4G were isolated. This study showed that Q3G 1 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 2 mg/kgBW significantly inhibited glucose uptake from rat intestinal membrane compared to negative control at 45th minute (p<0.05). Therefore, Q3G and Q4G isolated from mimba leaves have significantly inhibit glucose uptake from rat's intestinal membrane (Rattus ratt...

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“…Neem seed extract signifi cantly distorted the growth curve of clinical isolates of dermatophytes, T richophyton rubrum, Trichophyton mentagrophytes and Microsporum nanum in vitro at MIC of 15ug/mL (Natarajan et al 2003 ). The ethanol and ethyl acetate neem leaf extracts inhibited the growth of clinical isolates of dermatophytes isolated from human patients (Radhika and Michael 2013 ( Islam et al 2012 ). The antibiotic kanamycin was far more potent against all the test microorganisms.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Azadirachta indica

Lim¹

2014

Edible Medicinal and Non Medicinal Plants

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Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of <i>Azadirachta Indica</i> and Antimicrobial and Cytotoxic screening of the Crude Extracts

Cited by 24 publications

References 9 publications

Steroids and isoquercetin from Lygodium microphyllum

Steroids and isoquercetin from Lygodium microphyllum

Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa

Azadirachta indica

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