Isolation of replication-competent molecular clones of visna virus

Staskus, Katherine; Retzel, Ernest F.; Lewis, Eleanor T.; Silsby, J.L.; Cyr, S.T. Sheila; Rank, Jeffrey M.; Wietgrefe, Steven W.; Haase, Ashley T.; Cook, R. F.; Fast, David J.; Geiser, Paul T.; Harty, John T.; Kong, Selene H.; Lahti, Carol J.; Neufeld, Thomas P.; Porter, Thomas; Shoop, Elizabeth; Zachow, Karen R.

doi:10.1016/0042-6822(91)90488-w

Cited by 59 publications

(30 citation statements)

References 35 publications

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“…The neural adapted Icelandic K1514 strain of MVV was used (Staskus et al, 1991). One hundred thousand cells were infected with 100 TCID50 by incubation in serum-free DME for 2 h at 378C, followed by three rinses with serum-free DME and ®nally incubated in DME plus 2% FCS.…”

Section: Virus Infectionmentioning

confidence: 99%

Phenotypic characterisation and infection of ovine microglial cells with Maedi-Visna Virus

Ebrahimi¹,

Allsopp²,

Fazakerley³

et al. 2000

J Neurovirol

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Maedi-Visna Virus (MVV) infection of the central nervous system (CNS) results in pathological changes, the mechanisms of which are poorly understood. MVV preferentially infects cell of the monocyte/macrophage lineage in vivo. The neuroparenchymal microglial cells are the resident tissue macrophages in the CNS and therefore likely targets for MVV infection. However, no information is currently available on the susceptibility of these cells to MVV infection or their contribution to neuropathological changes as a result of MVV infection. Highly enriched primary ovine microglial cell cultures were set up from brain tissues of lambs. These cells were amoeboid or bipolar with spikes, a morphology consistent with microglial cells of other species, and stained positive for CD1, CD11a, CD11c, CD14, MHC-class I, MHC-class II, and b-N-acetyl galactose, but not with markers of astrocytes or oligodendrocytes. These sheep microglial cells were permissive for MVV infection. Productive MVV infection resulted in selective transcriptional upregulation of the pro-in¯ammatory cytokines TNFa and IL-6. In contrast, there was no change in levels of transcripts for TGFb1, IL-1b, GM-CSF, IL-10, or IL-12. These data provide the ®rst evidence that ovine microglial cells can support productive infection with MVV, and that this leads to a selective upregulation of proin¯ammatory cytokines. These may contribute to visna neuropathology. Journal of NeuroVirology (2000) 6, 320 ± 328.

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Section: Virus Infectionmentioning

confidence: 99%

Phenotypic characterisation and infection of ovine microglial cells with Maedi-Visna Virus

Ebrahimi¹,

Allsopp²,

Fazakerley³

et al. 2000

J Neurovirol

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“…Then, all samples indicating the presence of proviral DNA were subjected to two rounds nested PCR allowing amplification of a 122 bp fragment of gag gene, encoding immunodominant epitope on p25 capside protein. All primers were selected on the basis of published sequence of pLV1KS1 plasmid, according to the previously published data (17). PCR primers were designed within conserved sequences allowing amplification highly divergent prototypic K1514, CAEV-Co, and SRLVs field strains, respectively (3).…”

Section: Methodsmentioning

confidence: 99%

Diversity of GAG Gene Sequence Encoding Immunodominant Epitope on Capsid Protein of Lentiviruses from Sheep in Poland

Olech

Rola–Łuszczak

Kozaczynska

et al. 2012

Bulletin of the Veterinary Institute in Pulawy

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In the study, a 122 bp fragment of gag gene encoding immunodominant epitope on capsid protein of small ruminant lentiviruses (SRLVs) found in sheep was amplified by PCR and analysed by SSCP and sequencing. Out of 30 DNA samples, five showed different migration patterns, demonstrating the individual variations within gag sequences, which were confirmed afterwards by sequence analysis. In two samples nucleotide changes yielded amino acid substitutions highlighting the conservative nature of gag encoded immunoreactive epitope but also potencial insensitivity of a single-strain-based immunoassay.

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“…Plasmid pLVI-ILKS1 was a gift from K.A. Staskus (Staskus et al, 1991) and contains the complete provira[ genome of the MVV I514 clone inserted into the SaII site of pBR322. Plasmid pCAEV (Saltarelli et al, 1990) contains a large part of the proviral sequence of CAEV introduced into the HindllI site of pUC18.…”

Section: Methodsmentioning

confidence: 99%

Variations in Lentiviral Gene Expression in Monocyte-derived Macrophages from Naturally Infected Sheep

Chebloune

Karr

Sheffer

et al. 1996

Journal of General Virology

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Isolation of replication-competent molecular clones of visna virus

Cited by 59 publications

References 35 publications

Phenotypic characterisation and infection of ovine microglial cells with Maedi-Visna Virus

Phenotypic characterisation and infection of ovine microglial cells with Maedi-Visna Virus

Diversity of GAG Gene Sequence Encoding Immunodominant Epitope on Capsid Protein of Lentiviruses from Sheep in Poland

Variations in Lentiviral Gene Expression in Monocyte-derived Macrophages from Naturally Infected Sheep

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