Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b

Su, Pin; Feng, Tuizi; Zhou, Xuguo; Zhang, Songbai; Junfeng, Cheng; Luo, Yunzi; Peng, Jing; Zhang, Zhuo; Lu, Xiangyang; Zhang, Deyong; Liu, Yong

doi:10.1038/srep16121

Cited by 22 publications

(18 citation statements)

References 41 publications

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“…The TMV U1 strain was propagated and purified from its systemic host Nicotiana tabacum cv. Huangmiaoyu as described by Pin Su (Su et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Photosynthetic bacterium Rhodopseudomonas palustris GJ‐22 induces systemic resistance against viruses

Tan

et al. 2017

Microbial Biotechnology

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SummaryPhotosynthetic bacteria (PSB) have been extensively used in agriculture to promote plant growth and to improve crop quality. Their potential application in plant disease management, however, is largely overlooked. In this study, the PSB strain Rhodopseudomonas palustris GJ‐22 was investigated for its ability to induce resistance against a plant virus while promoting plant growth. In the field, a foliar spray of GJ‐22 suspension protected tobacco plants against tobacco mosaic virus (TMV). Under axenic conditions, GJ‐22 colonized the plant phyllosphere and induced resistance against TMV. Additionally, GJ‐22 produced two phytohormones, indole‐3‐acetic acid and 5‐aminolevulinic acid, which promote growth and germination in tobacco. Furthermore, GJ‐22‐inoculated plants elevated their immune response under subsequent TMV infection. This research may give rise to a novel biological agent with a dual function in disease management while promoting plant growth.

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“…The TMV U1 strain was propagated and purified from its systemic host Nicotiana tabacum cv. Huangmiaoyu as described by Pin Su (Su et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Photosynthetic bacterium Rhodopseudomonas palustris GJ‐22 induces systemic resistance against viruses

Tan

et al. 2017

Microbial Biotechnology

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“…Another representative of Alphaproteobateria, Rhodopseudomonas palustris, is a photosynthetic purple non-sulfur bacteria (Austin et al, 2015) exhibiting diverse biological activities. Su et al (2015) found an antiviral protein showing significant inhibitory activity against tobacco mosaic virus (TMV) in vivo and in vitro in the JSC-3b bacterial strain. Nookongbuta et al (2020) demonstrated that exopolymeric substances (EPS), lipopeptides and photopigments extracted from the KTSSR54 strain showed antifungal activity against three rice fungal pathogens.…”

Section: Discussionmentioning

confidence: 99%

PCR-screening of bacterial strains isolated from the microbiome of the Lubomirskia baicalensis sponge for the presence of secondary metabolite synthesis genes

Kaluzhnaya

Lipko

Ицкович

2021

LFWB

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Abstract. The microbial communities of sponges (Porifera) are often a source of natural bioactive metabolites. From the microbiome of the endemic Lubomirskia baicalensis sponge, 35 bacterial strains were isolated and identified using molecular methods. The strains belonged to the phyla Actinobacteria, Firmicutes, and Proteobacteria (classes Alpha- and Betapriteobacteria). To analyze the strains for the presence of genes in the synthesis of secondary metabolites, polyketide synthases (PKS), PCR screening was applied using degenerate primers. Overall, 15 out of 35 strains contained PCR products corresponding in size to a fragment of the ketosynthase domain of the PKS gene cluster. Thus, the proposed method is applicable for rapid screening of the potential ability of microorganisms of different taxonomic groups to produce secondary metabolites. The work contributes to the study of the taxonomic diversity of cultured microorganisms, potential producers of biologically active substances, isolated from the microbiomes of Baikal sponges.

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“…Previous studies have shown that extracellular proteins produced by beneficial microorganisms have antiviral activity. Such extracellular proteins include SAMP from S. marcescens strain S3, 21 protein elicitor PeBL1 from B. laterosporus strain A60, 28 and activator proteins from Rhodopseudomonas palustris strain JSC‐3b 19 …”

Section: Discussionmentioning

confidence: 99%

“…Additionally, isolating antiviral molecules from beneficial microorganisms is one of the important strategies to prevent and control virus diseases in plants 19 . For example, a novel polysaccharide composed of D‐mannose, D‐galactose and D‐glucose from the cultured filtrate of fungus Trichosporon confers a much stronger local but not systemic resistance against TMV through priming pathogen‐related defense genes 20 .…”

Section: Introductionmentioning

confidence: 99%

BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

Jiao

Shi

et al. 2021

Pest Management Science

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BACKGROUND Tobacco mosaic virus (TMV) is one of destructive plant viruses, causing serious economic losses in the world. Using antiviral proteins or elicitors to inhibit viral infection or promote plant immunity is one of the efficient strategies against TMV. Our previous study identified that the fermentation broth of Brevibacillus laterosporus strain B8 showed strong antiviral activity against TMV. However, the active antiviral ingredient is still unclear. RESULTS Here, BLB8 (B. laterosporus strain B8 protein, BLB8), an antiviral protein from B. laterosporus strain B8 was isolated and characterized. BLB8 showed protective, inactive and curative effects against TMV, and the inhibition rate reached up to 63%, 83% and 55%, respectively. BLB8 infiltrated around the infection site of the recombinant virus TMV‐GFP inhibited the systemic extend and movement of TMV. Pretreatment of the bottom leaves with BLB8 inhibited the spread and accumulation of TMV in upper systemic leaves. Furthermore, BLB8 caused hypersensitive response (HR) in a dose‐dependent way, promoted H2O2 accumulation, and induced the expression of defense‐relative genes in Nicotiana benthamiana. CONCLUSION The antiviral protein BLB8 from B. laterosporus strain B8 effectively inhibits TMV infection in inactivation, protective and curative effects through triggering plant immunity in tobacco. Therefore, the present study provides a new antiviral agent for prevention and control of viral disease. © 2021 Society of Chemical Industry.

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Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b

Cited by 22 publications

References 41 publications

Photosynthetic bacterium Rhodopseudomonas palustris GJ‐22 induces systemic resistance against viruses

Photosynthetic bacterium Rhodopseudomonas palustris GJ‐22 induces systemic resistance against viruses

PCR-screening of bacterial strains isolated from the microbiome of the Lubomirskia baicalensis sponge for the presence of secondary metabolite synthesis genes

BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

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