Isolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique

Abstract

Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra. The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary metabolites used column-chromatography with eluent of n-hexane : ethyl acetate (8:4). The results showed that secondary metabolites obtained from phytochemical tests were flavonoids, saponins and triterpenoids.

Anticancer Potential of Three Sea Cucumber Species Extracts on Human Breast Cancer Cell Line

“…Sukmiwati et al (2020) supported our study where their H. atra extract from Cerocok Beach, West Sumatera, Indonesia also presented a phenolic compound without flavonoid compound. However, another part of Indonesia was found absent with phenolic compound, but flavonoid was present in their H. atra extract from a different location at Benteng Inong, Aceh Besar, and Panjang Island, Jepara (Agustina et al, 2021;Sibero et al, 2019). Esmat et al (2013) found both compounds…”

“…Kartikaningsih et al (2018) stated that a flavonoid test was not done since it is a phytochemical presence in plants, whereas H. atra is an animal species. Little did we know, flavonoid phytochemicals can also be found in certain animal species (Agustina et al, 2021;Rapi et al, 2020). Other than that, phenolic compounds also found ubiquitously in plants exhibit potent antioxidant activity regulated through redox properties acting as reducing agents, singlet oxygen quenchers, hydrogen donors, and metal ion chelating agents (Gulcin, 2020;Kasote et al, 2015).…”

Antioxidant activity of silver nanoparticles synthesized using Holothuria atra extract

“…The change in the absorption intensity of this functional group indicates that there has been an interaction between bioactive compounds in the extract and silver solution to form silver nanoparticles. The research result by Agustina et al (2021) showed that the polyphenolic compounds contained in seacucumber extract (Holothuria atra) were flavonoids, saponins, and triterpenoids. According to Gurunathan et al (2009), hydroxide ions (OH-) play a vital role in accelerating the reduction reaction of silver ions in the formation of nanoparticles.…”